Product Overview
SHBC Q TopIEX BB is a Big Beads strong anion exchange chromatography media designed for high-flow biomolecule separation and purification.
The media is based on a highly crosslinked agarose matrix with an average particle size of approximately 200 μm. Its large-particle structure supports fast flow and reduces the risk of column blockage, making it particularly suitable for processing large sample volumes and samples with relatively low clarification.
Q TopIEX BB uses a Q (quaternary ammonium) functional group and acts as a strong anion exchanger.
Key Features
200 μm average particle size
Big Beads ion exchange chromatography media
Highly crosslinked agarose matrix
Q strong anion exchange ligand
180–250 μmol/mL (Cl⁻) ion loading capacity
Recommended flow rate: 90–450 cm/h
Pressure resistance: 0.3 MPa
Working pH range: 2–12
CIP pH range: 2–14
Fast flow characteristics
Reduced risk of column blockage
Suitable for large-volume sample processing
Suitable for relatively low-clarity samples
Technical Specifications
Parameter | Specification |
|---|
Product Name | Q TopIEX BB |
Brand | SHBC |
Product Type | Strong Anion Exchange Chromatography Media |
Matrix | Highly Crosslinked Agarose |
Ligand | Q (Quaternary Ammonium) |
Ion Exchange Type | Strong Anion Exchanger |
Average Particle Size | 200 μm |
Ion Loading Capacity | 180–250 μmol/mL (Cl⁻) |
Recommended Flow Rate | 90–450 cm/h |
Pressure Resistance | 0.3 MPa |
Working pH Range | 2–12 |
CIP pH Range | 2–14 |
Package | 25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L |
SHBC Art.No. | Packaging Specification |
A2068 | 25 mL |
A2069 | 100 mL |
A2070 | 300 mL |
A2071 | 1 L |
A2072 | 5 L |
A2073 | 10 L |
A2074 | 20 L |
Strong Anion Exchange Chromatography
Q TopIEX BB is a strong anion exchange chromatography media.
Ion exchange chromatography separates biomolecules according to differences in their surface charge under defined pH and buffer conditions.
The quaternary ammonium (Q) ligand provides strong anion exchange functionality. Under appropriate conditions, negatively charged biomolecules interact with the positively charged stationary phase and can subsequently be eluted by changing ionic strength or other chromatographic conditions.
Big Beads Design
Q TopIEX BB has an average particle size of approximately 200 μm.
The large-particle structure is designed to provide:
Faster mobile-phase flow
Lower risk of column blockage
Efficient processing of large sample volumes
Better tolerance for relatively low-clarity samples
Shorter processing time in high-throughput workflows
The BB series is designed for fast processing of large-volume samples and samples with relatively low clarification.
Highly Crosslinked Agarose Matrix
Q TopIEX BB uses a highly crosslinked agarose matrix.
The hydrophilic agarose matrix provides a suitable support for biomolecule chromatography, while the highly crosslinked structure supports the mechanical requirements of high-flow purification processes.
Q TopIEX BB has a specified pressure resistance of 0.3 MPa and a recommended linear flow rate of 90–450 cm/h.
High Ion Loading Capacity
Q TopIEX BB provides an ion loading capacity of:
180–250 μmol/mL (Cl⁻)
The combination of strong Q anion exchange functionality and Big Beads structure provides a balance between ion-exchange capacity and high-flow processing.
Wide pH Stability
Q TopIEX BB provides broad pH stability:
Working pH: 2–12
CIP pH: 2–14
This broad pH tolerance supports flexible process development and cleaning-in-place procedures.
Applications
SHBC Q TopIEX BB can be used for:
Protein purification
Recombinant protein purification
Biomolecule separation
Large-volume sample processing
Process-scale ion exchange chromatography
Capture purification
Intermediate purification
Biopharmaceutical downstream purification
Relatively low-clarity biological samples
Product Advantages
Q TopIEX BB combines:
Large 200 μm particle size
High-flow chromatography performance
Strong Q anion exchange functionality
High ion loading capacity
Broad pH stability
Reduced risk of column blockage
Efficient processing of large sample volumes
Highly crosslinked agarose matrix
Q TopIEX BB vs. Q TopIEX HP
Q TopIEX BB and Q TopIEX HP are designed for different purification priorities.
Feature | Q TopIEX BB | Q TopIEX HP |
Positioning | Big Beads / High Throughput | High Resolution |
Average Particle Size | 200 μm | 34 μm |
Main Advantage | Fast flow and reduced blockage | Higher separation resolution |
Typical Use | Large-volume processing | Intermediate / fine purification |
Q TopIEX BB is preferable when high throughput, rapid sample processing and resistance to blockage are key priorities.
Q TopIEX HP is more suitable when higher chromatographic resolution is required.
Storage and Handling
Keep the chromatography media in its original container and protect it from contamination.
Before use, equilibrate the packed bed with an appropriate buffer according to the purification process.
Operating conditions, loading amount, buffer composition, elution conditions and CIP procedures should be optimized according to the target biomolecule and process requirements.
Frequently Asked Questions
What is Q TopIEX BB?
Q TopIEX BB is a Big Beads strong anion exchange chromatography media based on a highly crosslinked agarose matrix.
What is the average particle size?
The average particle size is approximately 200 μm.
What ligand is used?
The functional ligand is Q (quaternary ammonium).
Is Q TopIEX BB a strong or weak anion exchanger?
It is a strong anion exchange chromatography media.
What is the ion loading capacity?
The ion loading capacity is 180–250 μmol/mL (Cl⁻).
What is the recommended flow rate?
The recommended flow rate is 90–450 cm/h.
What is the pressure resistance?
The specified pressure resistance is 0.3 MPa.
What is the working pH range?
The working pH range is 2–12.
What is the CIP pH range?
The CIP pH range is 2–14.
The large-particle structure supports faster flow, reduces the risk of column blockage and is suitable for processing large-volume and relatively low-clarity samples.
What package sizes are available?
SHBC offers 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L packages.
What are the SHBC Art.No. numbers?
The corresponding SHBC Art.No. are A2068, A2069, A2070, A2071, A2072, A2073 and A2074.
What brand is Q TopIEX BB?
Q TopIEX BB is supplied under the SHBC brand.
