Products
SHBC provides colored microspheres, fluorescent microspheres, magnetic beads, silica microspheres, chromatography packing microspheres and biological reagents for diagnostic assay development, nucleic acid extraction, protein purification and separation applications.
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TopGF 200
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SHBC
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6%
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34µm
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200ml 1L 5L 10L 20L
34µm TopGF 200 Agarose Gel Filtration Media
SHBC TopGF 200 is a high-resolution agarose gel filtration medium with an average particle size of 34µm.
It is developed for size exclusion separation, purification and analysis of monoclonal antibodies, large proteins, protein complexes and other biological macromolecules.
The fine particles provide efficient mass transfer, sharp chromatographic peaks and reliable polishing performance.
Product Overview
TopGF 200 is an agarose-based size exclusion chromatography resin designed for preparative protein purification.
Its porous and hydrophilic matrix separates biomolecules according to hydrodynamic size. The non-binding separation mechanism helps maintain protein structure and biological activity.
TopGF 200 is suitable for laboratory research, process development, antibody purification and industrial polishing processes.
Key Features and Benefits
Average particle size of 34µm
High-resolution size separation
Suitable for monoclonal antibodies
Effective protein aggregate removal
Separation of monomers, dimers and fragments
Hydrophilic agarose-based matrix
Low nonspecific adsorption
High biomolecule recovery
Sharp and reproducible peaks
Suitable for polishing chromatography
Reliable batch consistency
Laboratory and bulk supply available
Product Specifications
Parameter | Specification |
|---|---|
Product Name | TopGF 200 |
Brand | SHBC |
Product Category | Agarose gel filtration media |
Average Particle Size | 34µm |
Separation Mode | Size exclusion chromatography |
Alternative Name | GF200 chromatography resin |
Product Form | Gel filtration resin slurry |
Main Use | Protein separation, purification and analysis |
Recommended Samples | Antibodies, large proteins and protein complexes |
Manufacturer | Shanghai SanYu Biotechnology Co., Ltd. |
The exact fractionation range, pressure limit, pH stability and storage solution should be confirmed using the SHBC technical data sheet.
How TopGF 200 Works
TopGF 200 separates biomolecules according to their size and ability to enter the pores of the resin particles.
Large molecules have limited access to the internal pores and pass through the column more quickly. Smaller molecules enter more pores and elute later.
This size-based mechanism does not require a specific ligand or strong adsorption, providing gentle conditions for sensitive proteins.
Recommended Applications
Monoclonal Antibody Purification
Suitable for preparative separation, purity improvement and polishing of monoclonal antibody products.
Antibody Aggregate Removal
TopGF 200 can separate antibody monomers from dimers, oligomers and high-molecular-weight aggregates.
Protein Fragment Removal
Suitable for removing degradation fragments and low-molecular-weight protein impurities.
Large Protein Purification
Can be used for large recombinant proteins, enzymes, fusion proteins and other macromolecular protein products.
Protein Complex Separation
Suitable for separating protein complexes, oligomers and biomolecular assemblies according to size.
Biologic Drug Polishing
Can be used after affinity or ion exchange chromatography to improve final product purity.
Product Formulation
Suitable for protein sample cleanup, buffer-condition evaluation and formulation process development.
Quality Control Research
Can be used to evaluate protein aggregation, degradation, purity and molecular-size distribution.
Advantages of 34µm GF200 Media
High Separation Resolution
The fine 34µm particles form a uniform packed bed and improve separation between proteins with similar sizes.
Efficient Aggregate Removal
The porous matrix supports separation of antibody monomers from dimers and larger aggregates.
Sharp Chromatographic Peaks
Controlled particle size helps reduce peak broadening and produces concentrated target fractions.
Low Nonspecific Adsorption
The hydrophilic matrix reduces unwanted protein interactions and supports high sample recovery.
Gentle Purification
Size exclusion chromatography does not require harsh binding or elution conditions.
Suitable for Polishing
TopGF 200 is designed for purification stages where final product purity and aggregate removal are important.
TopGF 200 vs. TopGF 75
Feature | TopGF 75 | TopGF 200 |
|---|---|---|
Average Particle Size | 34µm | 34µm |
Main Target | Medium-sized proteins | Antibodies and large proteins |
Typical Application | Recombinant protein purification | Antibody polishing |
Aggregate Removal | Protein systems | Antibody and large-protein systems |
Separation Mode | Size exclusion | Size exclusion |
Main Advantage | Protein resolution | Large biomolecule resolution |
TopGF 75 is recommended for medium-sized recombinant proteins. TopGF 200 is more suitable for monoclonal antibodies, large proteins and protein complexes.
General Operating Procedure
Mix the resin slurry gently before use.
Remove the storage solution.
Wash the resin with purified water or equilibration buffer.
Prepare a uniform slurry.
Pack the chromatography column under controlled flow or pressure.
Equilibrate the packed bed with running buffer.
Clarify and filter the sample.
Apply the sample at the optimized flow rate.
Elute using the selected running buffer.
Collect and analyze the target fractions.
Clean and store the resin according to the validated procedure.
Sample volume, bed height, flow rate and buffer conditions should be optimized for the target protein and required resolution.
Column Packing Recommendations
Fully suspend the resin before packing.
Avoid introducing air bubbles.
Use filtered and degassed buffers.
Select a column frit suitable for 34µm particles.
Keep the slurry concentration uniform.
Do not allow the packed bed to dry.
Avoid sudden pressure changes.
Monitor column backpressure.
Evaluate packing efficiency before purification.
Why Choose SHBC TopGF 200
Controlled Particle Manufacturing
SHBC applies controlled particle preparation and classification processes to support consistent 34µm particle size.
Designed for Antibody Polishing
TopGF 200 is suitable for monoclonal antibody purification, aggregate removal and final polishing.
Reliable Batch Consistency
Controlled manufacturing and quality inspection support reproducible chromatographic performance.
Flexible Supply
Laboratory samples, pilot quantities and bulk resin packaging are available.
Process Scale-Up Support
SHBC supports method development, pilot evaluation and preparative purification projects.
Technical Service
Technical support is available for resin selection, column packing, sample loading and process optimization.
Custom and Bulk Supply
Shanghai SanYu Biotechnology Co., Ltd. supplies TopGF 200 to universities, research institutes, biotechnology companies, antibody manufacturers and pharmaceutical process developers.
Available services include:
Laboratory sample supply
Pilot-scale quantities
Bulk resin production
Custom packaging volume
Custom storage solution
Batch quality documentation
Column packing guidance
Antibody purification support
OEM and ODM manufacturing
Please provide the target protein, molecular weight, required purity, sample volume and estimated annual demand when requesting a quotation.
Frequently Asked Questions
What is TopGF 200?
TopGF 200 is a 34µm agarose gel filtration medium used for high-resolution separation of antibodies and large proteins according to molecular size.
What samples are suitable for TopGF 200?
Typical samples include monoclonal antibodies, large recombinant proteins, enzymes, protein complexes and other biological macromolecules.
Is TopGF 200 suitable for monoclonal antibodies?
Yes. It is suitable for antibody purification, aggregate removal, polishing and formulation research.
Can TopGF 200 remove antibody aggregates?
Yes. It can be evaluated for separating antibody monomers from dimers, oligomers and larger aggregates.
Can it remove protein fragments?
Yes. TopGF 200 can separate target proteins from low-molecular-weight degradation fragments.
Does TopGF 200 bind the target protein?
No specific ligand binding is required. Separation is mainly based on molecular size.
What is the difference between TopGF 200 and TopGF 75?
TopGF 75 is mainly used for medium-sized proteins, while TopGF 200 is designed for antibodies, large proteins and protein complexes.
Is TopGF 200 suitable for polishing chromatography?
Yes. Its fine particles and high-resolution performance make it suitable for intermediate and final polishing steps.
Can SHBC provide bulk quantities?
Yes. SHBC supports sample testing, pilot orders, customized packaging and bulk production.
Storage and Handling
Store TopGF 200 according to the conditions stated in the SHBC technical documentation.
Do not freeze or allow the resin to dry. Keep the container tightly closed and avoid microbial contamination. Mix gently before use and validate cleaning and storage conditions for the final process.
Request a Sample or Quotation
Contact Shanghai SanYu Biotechnology Co., Ltd. for samples, technical documentation, customized packaging or bulk pricing.
Product: TopGF 200
Brand: SHBC
Product Type: Agarose gel filtration media
Average Particle Size: 34µm
Separation Mode: Size exclusion chromatography
Main Applications: Antibody purification, aggregate removal and protein polishing
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
For research and industrial purification use.