Products
SHBC provides colored microspheres, fluorescent microspheres, magnetic beads, silica microspheres, chromatography packing microspheres and biological reagents for diagnostic assay development, nucleic acid extraction, protein purification and separation applications.
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A3043 / A3044 / A3045 / A3046 / A3047 / A3048 / A3049
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6% Cross-Linked Agarose
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Phenyl
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≈24 mg/mL (Human Serum Albumin)
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6%
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34 μm
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25mL 100mL 300mL 1L 5L 10L 20L
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Chromatography Grade
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High Performance Phenyl HIC Resin FF (LS)
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Protein Purification / Fine Purification / Hydrophobic Interaction Chromatography
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High-Resolution Hydrophobic Interaction Chromatography Media
Product Overview
SHBC Phenyl TopHIC FF (LS) is a high-resolution hydrophobic interaction chromatography (HIC) media designed for the separation and purification of proteins and other biomolecules.
The media is based on 6% cross-linked agarose with an average particle size of approximately 34 μm and uses a Phenyl hydrophobic ligand.
Phenyl TopHIC FF (LS) provides approximately 24 mg/mL dynamic binding capacity for human serum albumin and is suitable for intermediate and fine purification processes requiring high chromatographic resolution.
Key Features
34 μm average particle size
6% cross-linked agarose matrix
Phenyl hydrophobic ligand
LS specification
High-resolution HIC media
≈24 mg/mL dynamic binding capacity for Human Serum Albumin
Smaller particle size than conventional FF series
Higher chromatographic resolution
Suitable for intermediate purification
Suitable for fine purification
Designed for protein and biomolecule separation
Technical Specifications
Parameter | Specification |
|---|---|
Product Name | Phenyl TopHIC FF (LS) |
Brand | SHBC |
Product Type | High-Resolution Hydrophobic Interaction Chromatography Media |
Matrix | 6% Cross-Linked Agarose |
Ligand | Phenyl |
Specification | LS |
Chromatography Mode | Hydrophobic Interaction Chromatography |
Average Particle Size | 34 μm |
Dynamic Binding Capacity | ≈24 mg/mL (Human Serum Albumin) |
Package | 25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L |
Ordering Information
SHBC Art.No. | Packaging Specification |
A3043 | 25 mL |
A3044 | 100 mL |
A3045 | 300 mL |
A3046 | 1 L |
A3047 | 5 L |
A3048 | 10 L |
A3049 | 20 L |
34 μm High-Resolution Agarose Matrix
Phenyl TopHIC FF (LS) uses a 6% cross-linked agarose matrix with an average particle size of approximately 34 μm.
Compared with larger-particle fast-flow chromatography media, the smaller particle size provides improved chromatographic resolution.
The media is suitable for:
Intermediate purification
Fine purification
Polishing processes
High-resolution protein separation
Separation of biomolecules with different hydrophobic properties
Hydrophobic Interaction Chromatography
Hydrophobic interaction chromatography separates proteins and other biomolecules according to differences in their surface hydrophobicity.
Under suitable high-salt conditions, exposed hydrophobic regions of biomolecules interact with hydrophobic ligands immobilized on the chromatography matrix.
Changing the mobile-phase conditions can weaken these interactions and allow biomolecules with different hydrophobic characteristics to be separated.
Phenyl Hydrophobic Ligand
Phenyl TopHIC FF (LS) uses a Phenyl hydrophobic ligand.
Phenyl provides an aromatic hydrophobic interaction environment that differs from alkyl ligands such as Butyl and Octyl.
This different ligand chemistry can provide alternative chromatographic selectivity for proteins and other biomolecules.
LS Specification
This product is designated LS in the supplied product table.
The specified reference dynamic binding capacity is:
≈24 mg/mL (Human Serum Albumin)
Because the supplied table does not provide a separate technical definition for LS, the designation is retained as part of the product name without adding an unsupported interpretation.
Dynamic Binding Capacity
Phenyl TopHIC FF (LS) provides a specified reference dynamic binding capacity of:
≈24 mg/mL for Human Serum Albumin
Human Serum Albumin is used as the reference protein for the binding-capacity specification.
Actual binding performance can vary according to target molecule properties and chromatographic conditions.
High Resolution for Fine Purification
The average particle size is approximately 34 μm.
The smaller particle size is designed to provide higher chromatographic resolution, making Phenyl TopHIC FF (LS) suitable for purification processes requiring:
Improved separation resolution
Fine separation
Intermediate purification
Higher product purity
Final polishing
Applications
SHBC Phenyl TopHIC FF (LS) can be used for:
Protein purification
Recombinant protein purification
Biomolecule separation
Hydrophobic interaction chromatography
Intermediate purification
Fine purification
Polishing chromatography
Separation based on hydrophobicity
Biopharmaceutical downstream processing
Chromatography method development
Product Advantages
Phenyl TopHIC FF (LS) combines:
High chromatographic resolution
Fine 34 μm particle size
6% cross-linked agarose matrix
Phenyl hydrophobic selectivity
≈24 mg/mL Human Serum Albumin binding capacity
Suitable for intermediate purification
Suitable for fine purification
Suitable for protein and biomolecule separation
Phenyl TopHIC FF (LS) vs. Phenyl TopHIC FF (HS)
Both products use a Phenyl hydrophobic ligand and belong to the same high-resolution HIC product group.
Feature | Phenyl TopHIC FF (LS) | Phenyl TopHIC FF (HS) |
Ligand | Phenyl | Phenyl |
Matrix | 6% Cross-Linked Agarose | 6% Cross-Linked Agarose |
Average Particle Size | 34 μm | 34 μm |
Specification | LS | HS |
Reference Protein | Human Serum Albumin | Human Serum Albumin |
Dynamic Binding Capacity | ≈24 mg/mL | ≈36 mg/mL |
Main Difference in Supplied Data | Lower reference binding capacity | Higher reference binding capacity |
The supplied data distinguishes the two products by HS/LS designation and reference dynamic binding capacity.
For a specific target biomolecule, media selection should be confirmed through chromatographic screening and process optimization.
Phenyl vs. Butyl and Octyl HIC Media
Different HIC ligands can provide different chromatographic selectivity.
HIC Media | Ligand |
Phenyl TopHIC FF (LS) | Phenyl |
Butyl TopHIC HP | Butyl |
Octyl TopHIC HP | Octyl |
Phenyl contains an aromatic hydrophobic group, while Butyl and Octyl use alkyl hydrophobic groups.
Ligand screening can help determine which media provides the desired selectivity for a particular biomolecule.
Storage and Handling
Keep Phenyl TopHIC FF (LS) in its original container and protect the chromatography media from contamination during storage and handling.
Before use, equilibrate the packed bed with an appropriate buffer according to the intended HIC purification process.
Salt concentration, salt type, pH, sample loading and elution conditions should be optimized according to the target biomolecule.
Frequently Asked Questions
What is Phenyl TopHIC FF (LS)?
Phenyl TopHIC FF (LS) is a high-resolution hydrophobic interaction chromatography media using a Phenyl ligand.
What is the average particle size?
The average particle size is approximately 34 μm.
What matrix is used?
The media is based on 6% cross-linked agarose.
What ligand does Phenyl TopHIC FF (LS) use?
It uses a Phenyl hydrophobic ligand.
What is the dynamic binding capacity?
The specified dynamic binding capacity is approximately 24 mg/mL for Human Serum Albumin.
What does LS mean?
The supplied product table identifies this product as Phenyl TopHIC FF (LS) but does not separately define LS. Therefore, LS is retained as the product specification designation.
What is the difference between Phenyl TopHIC FF (LS) and FF (HS)?
According to the supplied table, both use Phenyl ligands and have a 34 μm high-resolution matrix. The listed dynamic binding capacity is approximately 24 mg/mL for LS and 36 mg/mL for HS.
Is Phenyl TopHIC FF (LS) suitable for fine purification?
Yes. The 34 μm high-resolution series is designed for intermediate and fine purification of biomolecules.
What applications is this media suitable for?
Typical applications include protein purification, biomolecule separation, intermediate purification, fine purification and hydrophobic interaction chromatography.
What package sizes are available?
SHBC offers 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L packages.
What are the SHBC Art.No. numbers?
The corresponding SHBC Art.No. are A3043, A3044, A3045, A3046, A3047, A3048 and A3049.
What brand is Phenyl TopHIC FF (LS)?
Phenyl TopHIC FF (LS) is supplied under the SHBC brand.