Product Overview
SHBC Phenyl TopHIC HP is a high-performance, high-resolution hydrophobic interaction chromatography (HIC) media designed for separation and purification of proteins and other biomolecules.
The media is based on 6% cross-linked agarose, with an average particle size of approximately 34 μm, and uses a Phenyl hydrophobic ligand.
Its smaller particle size provides higher chromatographic resolution than the FF series, making Phenyl TopHIC HP particularly suitable for intermediate and fine purification of biomolecules.
Key Features
34 μm average particle size
6% cross-linked agarose matrix
Phenyl hydrophobic ligand
High-performance HIC media
≈45 mg/mL dynamic binding capacity for α-chymotrypsinogen
Smaller particle size than FF series
Higher chromatographic resolution
Suitable for intermediate purification
Suitable for fine purification
Designed for protein and biomolecule separation
Technical Specifications
Parameter | Specification |
|---|
Product Name | Phenyl TopHIC HP |
Brand | SHBC |
Product Type | High-Resolution Hydrophobic Interaction Chromatography Media |
Matrix | 6% Cross-Linked Agarose |
Ligand | Phenyl |
Chromatography Mode | Hydrophobic Interaction Chromatography |
Average Particle Size | 34 μm |
Dynamic Binding Capacity | ≈45 mg/mL (α-Chymotrypsinogen) |
Package | 25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L |
SHBC Art.No. | Packaging Specification |
A3050 | 25 mL |
A3051 | 100 mL |
A3052 | 300 mL |
A3053 | 1 L |
A3054 | 5 L |
A3055 | 10 L |
A3056 | 20 L |
34 μm High-Resolution Agarose Matrix
Phenyl TopHIC HP uses a 6% cross-linked agarose matrix with an average particle size of approximately 34 μm.
According to the supplied product information, the smaller particle size provides higher resolution than the FF series.
This makes Phenyl TopHIC HP suitable for:
Intermediate purification
Fine purification
High-resolution protein separation
Biomolecule purification
Purification processes requiring improved separation resolution
Hydrophobic Interaction Chromatography
Hydrophobic interaction chromatography separates biomolecules according to differences in their hydrophobic properties.
Phenyl TopHIC HP provides a hydrophobic stationary phase for protein and biomolecule separation and can be used as part of intermediate or fine purification workflows.
Chromatographic conditions should be optimized according to the properties of the target biomolecule.
Phenyl Hydrophobic Ligand
Phenyl TopHIC HP uses a Phenyl hydrophobic ligand.
The Phenyl ligand provides hydrophobic interaction selectivity for proteins and other biomolecules.
Combined with the fine 34 μm agarose matrix, the media is designed to provide high-resolution separation for demanding purification applications.
Dynamic Binding Capacity
The specified reference dynamic binding capacity of Phenyl TopHIC HP is:
≈45 mg/mL (α-Chymotrypsinogen)
α-Chymotrypsinogen is used as the reference biomolecule for the dynamic binding capacity listed in the supplied product table.
Actual binding capacity may vary depending on the target biomolecule and chromatographic operating conditions.
High Resolution for Fine Purification
Phenyl TopHIC HP belongs to the High Performance high-resolution HIC series.
The series uses 34 μm particles, which are smaller than those used in the FF series.
The smaller particle size provides improved chromatographic resolution and makes the media suitable for:
Intermediate purification
Fine purification
Improved peak resolution
Separation of biomolecules with different hydrophobic properties
High-purity protein purification workflows
Applications
SHBC Phenyl TopHIC HP can be used for:
Protein purification
Recombinant protein purification
Biomolecule separation
Hydrophobic interaction chromatography
Intermediate purification
Fine purification
High-resolution chromatography
Separation based on hydrophobic properties
Biopharmaceutical downstream processing
Chromatography method development
Product Advantages
Phenyl TopHIC HP combines:
High chromatographic resolution
Fine 34 μm particle size
6% cross-linked agarose matrix
Phenyl hydrophobic selectivity
≈45 mg/mL α-chymotrypsinogen binding capacity
Suitable for intermediate purification
Suitable for fine purification
Suitable for protein and biomolecule separation
Phenyl TopHIC HP vs. FF Series
Phenyl TopHIC HP belongs to the High Performance series and is designed with a smaller particle size than the FF series.
Feature | Phenyl TopHIC HP | FF Series |
Series | High Performance | Fast Flow |
Matrix | 6% Cross-Linked Agarose | FF Agarose Matrix |
Average Particle Size | 34 μm | Larger than HP Series |
Main Positioning | High Resolution | Fast Flow |
Typical Application | Intermediate / Fine Purification | General / Process Purification |
Main Advantage | Higher chromatographic resolution | Higher processing throughput |
Choose Phenyl TopHIC HP when chromatographic resolution and fine purification are the main priorities.
Available Package Sizes
Phenyl TopHIC HP is available in multiple package sizes for method development and different purification scales:
25 mL
100 mL
300 mL
1 L
5 L
10 L
20 L
This allows users to select an appropriate volume for laboratory evaluation, process development and larger-scale purification.
Storage and Handling
Keep Phenyl TopHIC HP in its original container and protect the chromatography media from contamination during storage and handling.
Before use, equilibrate the chromatography media according to the intended HIC purification process.
Sample loading and chromatographic conditions should be optimized according to the properties of the target biomolecule.
Frequently Asked Questions
What is Phenyl TopHIC HP?
Phenyl TopHIC HP is a high-performance, high-resolution hydrophobic interaction chromatography media based on 6% cross-linked agarose.
What is the average particle size?
The average particle size is approximately 34 μm.
What matrix is used?
The media uses a 6% cross-linked agarose matrix.
What ligand does Phenyl TopHIC HP use?
It uses a Phenyl hydrophobic ligand.
What is the dynamic binding capacity?
The specified reference dynamic binding capacity is approximately 45 mg/mL for α-chymotrypsinogen.
What does HP mean?
HP refers to the High Performance series, which uses smaller particles to provide higher chromatographic resolution than the FF series.
Is Phenyl TopHIC HP suitable for fine purification?
Yes. According to the supplied product information, the 34 μm high-resolution series is suitable for intermediate or fine purification of biomolecules.
Is Phenyl TopHIC HP suitable for protein purification?
Yes. It is designed for hydrophobic interaction separation and purification of proteins and other biomolecules.
What is the difference between the HP and FF series?
The supplied information states that the HP series has a smaller particle size and higher resolution than the FF series.
What package sizes are available?
SHBC offers 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L packages.
What are the SHBC Art.No. numbers?
The corresponding SHBC Art.No. are A3050, A3051, A3052, A3053, A3054, A3055 and A3056.
What brand is Phenyl TopHIC HP?
Phenyl TopHIC HP is supplied under the SHBC brand.
