Product Overview
SHBC HardIEX MA Fine is a high-rigidity, high-resolution multimodal anion exchange chromatography media designed for intermediate and fine purification of monoclonal antibodies, proteins and other biomolecules.
The HardIEX-Fine series is produced by coupling functional ligands onto a fine-particle high-rigidity agarose matrix.
With an average particle size of approximately 40 μm, HardIEX MA Fine offers higher chromatographic resolution than the standard HardIEX series and is suitable for purification stages where improved separation performance is required.
Key Features
Fine-particle high-rigidity agarose matrix
Approximately 40 μm average particle size
High-resolution chromatography media
Multimodal anion exchange functionality
45–85 mg/mL dynamic binding capacity for monoclonal antibody
Recommended flow rate: 90–150 cm/h
Pressure resistance: 0.5 MPa / 5 bar
Working pH range: 3–12
CIP pH range: 2–14
Higher resolution than standard HardIEX media
Suitable for intermediate purification
Suitable for fine purification and polishing
High mechanical stability
Technical Specifications
Parameter | Specification |
|---|
Product Name | HardIEX MA Fine |
Brand | SHBC |
Product Type | High-Resolution Multimodal Anion Exchange Chromatography Media |
Matrix | Fine-Particle High-Rigidity Agarose |
Ligand | MA Multimodal Anion Exchange Ligand |
Exchange Type | Multimodal Anion Exchange |
Average Particle Size | Approx. 40 μm |
Dynamic Binding Capacity | 45–85 mg/mL (Monoclonal Antibody) |
Recommended Flow Rate | 90–150 cm/h |
Pressure Resistance | 0.5 MPa / 5 bar |
Working pH Range | 3–12 |
CIP pH Range | 2–14 |
Package | 25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L |
SHBC Art.No. | Packaging Specification |
A2145 | 25 mL |
A2146 | 100 mL |
A2147 | 300 mL |
A2148 | 1 L |
A2149 | 5 L |
A2150 | 10 L |
A2151 | 20 L |
High-Rigidity High-Resolution Matrix
HardIEX MA Fine uses a fine-particle high-rigidity agarose matrix.
The Fine series has an average particle size of approximately 40 μm, which provides higher chromatographic resolution than the standard HardIEX platform.
This makes HardIEX MA Fine suitable for:
Intermediate purification
Fine purification
Polishing chromatography
Separation of closely related biomolecules
High-resolution bioprocess applications
The supplier specifically positions the HardIEX-Fine series for higher-resolution purification compared with the standard Hard series.
Multimodal Anion Exchange Chromatography
HardIEX MA Fine uses an MA multimodal anion exchange ligand.
Compared with conventional single-mode anion exchange media, multimodal media can provide additional selectivity through more than one interaction mechanism.
This makes HardIEX MA Fine suitable for purification processes where conventional charge-based separation alone may not provide sufficient selectivity.
High Dynamic Binding Capacity for Monoclonal Antibodies
HardIEX MA Fine provides a specified dynamic binding capacity of:
45–85 mg/mL for monoclonal antibody
This combines substantial antibody loading capacity with the enhanced separation resolution of the Fine series.
40 μm Particle Size for Higher Resolution
The average particle size of HardIEX MA Fine is approximately:
40 μm
The smaller particle size improves chromatographic efficiency and helps provide higher resolution than larger-particle process media.
HardIEX MA Fine is therefore particularly suitable when fine separation and polishing performance are priorities.
Recommended Flow Rate
The recommended linear flow rate is:
90–150 cm/h
This range balances resolution and process throughput.
Compared with standard HardIEX MA, the Fine version prioritizes higher chromatographic resolution rather than maximum flow rate.
Pressure Resistance up to 5 Bar
HardIEX MA Fine retains the mechanical advantages of the high-rigidity agarose platform.
The specified pressure resistance is:
0.5 MPa / 5 bar
This supports stable packed-bed operation during demanding intermediate and fine purification processes.
pH Stability
HardIEX MA Fine provides:
Working pH: 3–12
CIP pH: 2–14
The broad pH range supports flexible process development and cleaning-in-place procedures.
Applications
SHBC HardIEX MA Fine can be used for:
Monoclonal antibody purification
Protein purification
Intermediate purification
Fine purification
Polishing chromatography
Multimodal anion exchange chromatography
Biopharmaceutical downstream processing
Separation of closely related biomolecules
Chromatography method development
Purification process optimization
Product Advantages
HardIEX MA Fine combines:
High chromatographic resolution
Fine 40 μm particle size
High-rigidity agarose matrix
Multimodal anion exchange selectivity
45–85 mg/mL mAb binding capacity
Pressure resistance up to 5 bar
Broad pH stability
Suitable for intermediate and fine purification
Suitable for antibody polishing workflows
HardIEX MA Fine vs. HardIEX MA
HardIEX MA Fine and standard HardIEX MA are designed for different purification priorities.
Feature | HardIEX MA Fine | HardIEX MA |
Main Positioning | High Resolution / Fine Purification | High-Flow Multimodal Purification |
Average Particle Size | Approx. 40 μm | Approx. 90 μm |
Matrix | Fine-Particle High-Rigidity Agarose | High-Rigidity Agarose |
Separation Mode | Multimodal Anion Exchange | Multimodal Anion Exchange |
Recommended Flow Rate | 90–150 cm/h | 90–500 cm/h |
Pressure Resistance | 0.5 MPa | 0.5 MPa |
Reference Binding Capacity | 45–85 mg/mL mAb | ≥30 mg/mL BSA |
Main Advantage | Higher resolution and polishing performance | Faster flow and high-throughput purification |
Choose HardIEX MA Fine when higher resolution and fine purification performance are more important.
Choose standard HardIEX MA when higher flow rate and throughput are the primary priorities.
Storage and Handling
Keep the chromatography media in its original container and protect it from contamination during storage and handling.
Before use, equilibrate the packed bed with an appropriate buffer according to the intended purification process.
Sample pH, conductivity, loading amount, flow rate, elution conditions and CIP procedures should be optimized according to the target molecule and desired separation performance.
Frequently Asked Questions
What is HardIEX MA Fine?
HardIEX MA Fine is a high-rigidity, high-resolution multimodal anion exchange chromatography media designed for intermediate and fine purification.
What is the average particle size?
The average particle size is approximately 40 μm.
What is the dynamic binding capacity?
The specified dynamic binding capacity is 45–85 mg/mL for monoclonal antibody.
Is HardIEX MA Fine suitable for monoclonal antibody purification?
Yes. Monoclonal antibody is the reference molecule used for the specified dynamic binding capacity.
It is a high-resolution multimodal anion exchange chromatography media.
Why is the Fine series higher resolution?
The Fine series uses smaller high-rigidity agarose particles, with an average particle size of approximately 40 μm, providing improved chromatographic resolution.
What is the recommended flow rate?
The recommended flow rate is 90–150 cm/h.
What is the pressure resistance?
The specified pressure resistance is 0.5 MPa / 5 bar.
What is the working pH range?
The working pH range is 3–12.
What is the CIP pH range?
The CIP pH range is 2–14.
When should HardIEX MA Fine be selected instead of HardIEX MA?
HardIEX MA Fine is preferred when higher resolution, fine purification or polishing performance is required, while standard HardIEX MA is more suitable for higher-flow purification.
What package sizes are available?
SHBC offers 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L packages.
What are the SHBC Art.No. numbers?
The corresponding SHBC Art.No. are A2145, A2146, A2147, A2148, A2149, A2150 and A2151.
What brand is HardIEX MA Fine?
HardIEX MA Fine is supplied under the SHBC brand.
