50nm Silica Microspheres Si50NM-10 2%

50nm Silica Microspheres for Immunoassay Development

SHBC Si50NM-10 Silica Microspheres are uniform 50 nm silica nanoparticles supplied as a 2% suspension.

Designed for immunoassay research and diagnostic reagent development, these silica microspheres provide a high surface area for biomolecule adsorption, surface modification and nanoscale carrier preparation.

Si50NM-10 is suitable for laboratory evaluation, pilot-scale development and bulk production.

Product Specifications

Key Features and Advantages

High Surface Area

The 50 nm particle size provides a large surface area for biomolecule adsorption, surface modification and nanoparticle-based assay development.

Silanol-Rich Surface

The silica surface contains silanol groups that support physical adsorption and further silane-based functionalization.

Good Hydrophilicity

The hydrophilic silica matrix supports dispersion in aqueous research systems and compatibility with commonly used biological buffers.

Low Optical Background

Silica has low intrinsic fluorescence, making it suitable for fluorescence-related assay development and biosensor research.

Flexible Surface Modification

The surface can be further modified to introduce amino, carboxyl, epoxy or other functional groups according to the application.

Stable Batch Supply

SHBC supports sample testing, pilot-scale evaluation and bulk manufacturing for biotechnology companies and diagnostic reagent developers.

Why Choose 50nm Silica Microspheres?

The 50 nm size provides a balance between high surface area and practical dispersion performance.

Compared with larger silica microspheres, 50 nm particles offer more available surface area per unit mass. Compared with smaller nanoparticles, they can be easier to evaluate in many adsorption, modification and formulation studies.

Si50NM-10 can be used when the research project requires:

• High biomolecule loading potential

• Nanoscale carrier materials

• Flexible surface chemistry

• Aqueous dispersion

• Low intrinsic fluorescence

• Reproducible particle-based formulations

• Scale-up from research to batch production

Final performance should be verified under the customer’s specific buffer, protein, antibody and assay conditions.

Applications in Immunoassay Research

SHBC 50nm Silica Microspheres can be evaluated for:

• Antigen adsorption

• Antibody adsorption

• Protein immobilization

• Peptide carrier development

• Immunoassay signal carrier preparation

• Fluorescent probe development

• Biosensor surface development

• Diagnostic reagent formulation

• Nanoparticle labeling research

• Biomolecule conjugation studies

• Surface chemistry screening

• Nucleic acid detection research

• Multiplex assay development

• In vitro diagnostic raw material research

Biomolecule Adsorption and Surface Modification

The native silica surface can interact with proteins and other biomolecules through physical adsorption.

Adsorption performance depends on several factors, including:

• Buffer pH

• Ionic strength

• Protein isoelectric point

• Protein concentration

• Incubation time

• Microsphere concentration

• Blocking reagent

• Storage conditions

For applications requiring stronger and more stable binding, Si50NM-10 can be used as a base material for silanization and covalent surface modification.

Functional groups that may be introduced include:

• Amino groups

• Carboxyl groups

• Epoxy groups

• Aldehyde groups

• Thiol groups

• Biotin or streptavidin systems

The functionalization method should be selected according to the target biomolecule and assay format.

Recommended Handling

Mix the suspension gently before use to obtain a uniform dispersion.

For laboratory testing:

• Avoid allowing the microspheres to dry.

• Use clean and low-binding containers.

• Avoid excessive high-speed vortexing.

• Optimize sonication time according to the sample volume.

• Prevent overheating during ultrasonic dispersion.

• Evaluate buffer compatibility before large-scale use.

• Confirm particle stability after adding salts, proteins or surfactants.

The final operating conditions should be optimized for each immunoassay system.

Customization and Bulk Manufacturing

Shanghai SanYu Biotechnology Co., Ltd. provides silica microsphere development and manufacturing services for research institutions, biotechnology companies and diagnostic reagent manufacturers.

Available customization may include:

• Custom particle size

• Custom solid content

• Custom dispersion medium

• Amino surface modification

• Carboxyl surface modification

• Other functional groups

• Custom packaging volume

• Pilot-scale production

• Bulk manufacturing

• OEM and ODM services

Customization feasibility depends on the required particle size, concentration, surface chemistry, dispersion medium and order volume.

Why Choose SHBC?

• Specialized microsphere manufacturer

• Controlled 50 nm particle size

• Convenient 2% suspension

• Suitable for immunoassay research

• Flexible silica surface chemistry

• Support for physical adsorption studies

• Surface functionalization options

• Stable batch-to-batch production

• Sample and pilot-order support

• Bulk manufacturing capability

• OEM and ODM customization

• Technical support for assay development

Quality Documentation

Batch-related documentation can be provided according to product and order requirements.

Available documents may include:

• Certificate of Analysis

• Safety Data Sheet

• Product specification

• Particle size information

• Batch identification

• Storage and handling guidance

Please contact SHBC to confirm the available testing data and documentation for the required batch.

Frequently Asked Questions

What is Si50NM-10?

Si50NM-10 is a 50 nm silica microsphere suspension with a solid content of 2%, manufactured by Shanghai SanYu Biotechnology Co., Ltd. under the SHBC brand.

What are 50nm silica microspheres used for?

They can be evaluated for immunoassay research, biomolecule adsorption, protein immobilization, biosensors, surface modification and diagnostic reagent development.

Can antibodies be physically adsorbed onto the microspheres?

Yes. Physical adsorption can be evaluated using the native silica surface. Binding performance depends on the antibody, buffer pH, ionic strength and blocking conditions.

Can the silica surface be functionalized?

Yes. The surface silanol groups provide a foundation for silane chemistry and the introduction of amino, carboxyl, epoxy and other functional groups.

Why use 50nm silica microspheres?

The 50 nm particle size provides a high surface-to-volume ratio and is suitable for nanoscale carrier development, biomolecule loading and surface chemistry research.

Are the microspheres supplied as a dry powder?

Si50NM-10 is supplied as a 2% microsphere suspension. Custom formulations can be discussed according to project requirements.

Can SHBC provide bulk quantities?

Yes. SHBC supports laboratory samples, pilot-scale orders and bulk production for research and reagent development customers.

Can the particle size or concentration be customized?

Custom particle sizes, solid contents, surface groups, dispersion media and packaging formats can be discussed.

Is Si50NM-10 a finished diagnostic reagent?

No. It is a raw material intended for research and diagnostic reagent development. Customers should perform application-specific validation before commercial use.

Request Samples and Bulk Pricing

Contact SHBC for samples, technical specifications, customized silica microspheres and bulk quotations.

Please provide the following information when requesting a quotation:

• Required quantity

• Target application

• Preferred packaging

• Required surface chemistry

• Dispersion medium

• Expected annual demand

Product: 50nm Silica Microspheres
Catalog Number: Si50NM-10
Particle Size: 50 nm
Solid Content: 2%
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
Intended Use: Research Use Only