Product Overview
SHBC TopAC G is a 90 μm Protein G affinity chromatography resin designed for antibody and antibody fragment purification.
According to the supplied product information, TopAC G is prepared by immobilizing Protein G onto a Top-4FF matrix through cyanogen bromide activation.
Compared with Protein A, Protein G provides a broader antibody binding spectrum. Protein G binds strongly to the Fc region of antibodies and can also exhibit interactions with Fab fragments.
This makes TopAC G suitable for purification of antibodies and antibody fragments from cell culture, as well as antibody purification from sera of various species.
Key Features
90 μm particle size
Protein G affinity ligand
Top-4FF chromatography matrix
Broader antibody binding spectrum than Protein A
Strong interaction with antibody Fc regions
Interaction with antibody Fab fragments
Suitable for antibody purification
Suitable for antibody fragment purification
Suitable for antibodies from cell culture
Suitable for antibodies from sera of various species
Package sizes from 25 mL to 20 L
Technical Specifications
Parameter | Specification |
|---|
Product Name | TopAC G |
Brand | SHBC |
English Name | Protein G Affinity Chromatography Resin |
Matrix | Top-4FF Matrix |
Ligand | Protein G |
Particle Size | 90 μm |
Chromatography Mode | Affinity Chromatography |
Main Target | Antibodies / Antibody Fragments |
Main Applications | Cell Culture and Serum Antibody Purification |
Package | 25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L |
SHBC Art.No. | Packaging Specification |
|---|
A5008 | 25 mL |
A5009 | 100 mL |
A5010 | 300 mL |
A5011 | 1 L |
A5012 | 5 L |
A5013 | 10 L |
A5014 | 20 L |
Protein G Affinity Ligand
TopAC G uses Protein G as the affinity ligand.
According to the supplied product information, Protein G has a broader antibody binding spectrum compared with Protein A.
Protein G strongly binds to the Fc region of antibodies and can also exhibit interactions with antibody Fab fragments.
These binding characteristics make Protein G affinity chromatography useful for purification applications involving different antibodies and antibody fragments.
Top-4FF Matrix
The Protein G ligand is immobilized onto a Top-4FF matrix.
According to the supplied product description, the media is prepared through cyanogen bromide activation, followed by immobilization of Protein G onto the Top-4FF matrix.
The combination forms the affinity chromatography medium used for antibody separation and purification.
Broad Antibody Binding Spectrum
One of the main characteristics described for Protein G is its broader antibody binding spectrum compared with Protein A.
This can make TopAC G useful when the antibody of interest does not provide the desired interaction with Protein A affinity media.
The appropriate affinity ligand should be selected according to the antibody type and purification requirements.
Fc and Fab Interaction
Protein G has strong affinity toward the Fc region of antibodies.
The supplied product information also states that Protein G exhibits interactions with Fab fragments.
This enables TopAC G to be considered for both:
Antibody Purification from Cell Culture
TopAC G is commonly used for the separation and purification of antibodies or antibody fragments from cell culture samples.
Protein G affinity provides selective antibody interaction, supporting antibody isolation from complex biological samples.
Antibody Purification from Serum
TopAC G can also be used for purification of antibodies from the sera of various species.
Its broad antibody binding spectrum makes Protein G affinity chromatography useful for applications involving antibodies originating from different species.
Applications
SHBC TopAC G can be used for:
Antibody purification
Immunoglobulin purification
Protein G affinity chromatography
Antibody fragment purification
Fc-containing antibody purification
Cell culture antibody purification
Cell culture antibody fragment purification
Serum antibody purification
Antibody purification from different species
Affinity chromatography research
Product Advantages
TopAC G provides:
90 μm chromatography resin
Protein G affinity ligand
Top-4FF matrix
Broad antibody binding spectrum
Strong Fc interaction
Fab interaction
Suitable for intact antibodies
Suitable for antibody fragments
Suitable for cell culture samples
Suitable for sera from various species
Package sizes up to 20 L
Protein G vs. Protein A Affinity Chromatography
According to the supplied product information, an important distinction between Protein G and Protein A is antibody binding spectrum.
Feature | TopAC G | HardAC A |
|---|
Affinity Ligand | Protein G | Protein A |
Particle Size | 90 μm | 75 μm |
Matrix | Top-4FF | Highly Rigid Agarose |
Antibody Binding Spectrum | Broader than Protein A | Protein A Selectivity |
Fc Interaction | Strong | Yes |
Fab Interaction | Can interact with Fab fragments | Not specified in supplied data |
Main Application | Antibodies / Antibody Fragments | Monoclonal Antibodies / Fc-Fusion Proteins |
Serum Antibody Purification | Suitable | Not specified in supplied data |
The appropriate affinity resin should be selected according to antibody species, antibody type and purification requirements.
Available Package Sizes
TopAC G is available in:
25 mL
100 mL
300 mL
1 L
5 L
10 L
20 L
This range supports laboratory antibody purification, method development and larger-volume purification applications.
Frequently Asked Questions
What is TopAC G?
TopAC G is a 90 μm Protein G affinity chromatography resin designed for antibody and antibody fragment purification.
What is the particle size of TopAC G?
The specified particle size is 90 μm.
What ligand does TopAC G use?
TopAC G uses Protein G as its affinity ligand.
What matrix does TopAC G use?
According to the supplied product information, Protein G is immobilized onto a Top-4FF matrix.
How is Protein G immobilized?
The supplied information states that Protein G is immobilized onto the Top-4FF matrix through cyanogen bromide activation.
How does Protein G differ from Protein A?
According to the supplied information, Protein G has a broader antibody binding spectrum than Protein A.
Does Protein G bind to the Fc region of antibodies?
Yes. The supplied product information states that Protein G strongly binds to the Fc region of antibodies.
Can TopAC G be used for antibody fragments?
Yes. The supplied information indicates that Protein G also interacts with antibody Fab fragments, making TopAC G suitable for antibody fragment purification.
Can TopAC G purify antibodies from cell culture?
Yes. It is suitable for separating and purifying antibodies and antibody fragments from cell culture samples.
Can TopAC G be used for serum antibody purification?
Yes. It can be used to purify antibodies from the sera of various species.
What package sizes are available?
Available packages are 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L.
What are the SHBC Art.No. numbers?
The corresponding SHBC Art.No. are A5008, A5009, A5010, A5011, A5012, A5013 and A5014.
What is the English product name?
The supplied English name is Protein G Affinity Chromatography Resin.
What brand is TopAC G?
TopAC G is supplied under the SHBC brand.
