SHBC colored microspheres are obtained by dyeing latex white microspheres, filled with nearly 20% oil soluble dyes in the matrix inside the microspheres. The color is bright and stable, which can present different color bands for different detection items of the same test strip and solve the problem of mutual interference when multiple items are tested simultaneously. This product not only improves the sensitivity of detection reagents, but also solves the problem of instability between batches (size, color difference) of microspheres during the production process. It can be used for qualitative and quantitative detection