Agarose Series Gel Filter Packing CL-6B TopGF CL-6B 90um

TopGF CL-6B 90µm Agarose Gel Filtration Media

SHBC TopGF CL-6B is a 90µm cross-linked agarose gel filtration medium for the separation, purification and analysis of biological macromolecules.

The stable porous matrix supports size-based separation of polysaccharides, nucleic acids, viruses and other biomolecules. TopGF CL-6B can withstand high-temperature sterilization at 121°C under validated conditions.

Product Overview

TopGF CL-6B is an agarose-based size exclusion chromatography resin developed for laboratory research, process development and industrial purification.

The cross-linked matrix provides improved physical and chemical stability compared with conventional non-cross-linked agarose gels. Its 90µm average particle size supports convenient column packing, stable flow and reliable chromatographic operation.

Key Features and Benefits

• 90µm average particle size

• Cross-linked agarose matrix

• Size-based, non-binding separation

• Withstands sterilization at 121°C

• Good physical and chemical stability

• Stable column packing performance

• Suitable for biological macromolecules

• Gentle separation conditions

• Laboratory and bulk supply available

• Reliable batch-to-batch consistency

Product Specifications

How TopGF CL-6B Works

TopGF CL-6B separates molecules according to their hydrodynamic size.

Large molecules cannot fully enter the pores of the agarose beads and therefore pass through the column more quickly. Smaller molecules enter more pores, travel through a longer path and elute later.

This separation mechanism does not require specific chemical binding. It is suitable for gentle fractionation of sensitive biological samples. In gel filtration chromatography, larger molecules generally elute before smaller molecules because of differences in pore accessibility. ed Applications

Polysaccharide Purification

Suitable for the fractionation and purification of natural, pharmaceutical and functional polysaccharides.

Nucleic Acid Separation

Can be used for DNA, RNA and other nucleic acid purification, cleanup and analytical fractionation.

Virus Purification

Suitable for virus particle separation, vaccine process research and virus-related analytical applications.

Biological Macromolecule Fractionation

Used to separate proteins, protein complexes and other biomolecules according to molecular size.

Sample Cleanup

Can be used to remove small-molecule impurities from biological macromolecule samples.

Detection and Analysis

Suitable for size-based sample fractionation before downstream detection, characterization or quality analysis.

Advantages of Cross-Linked Agarose Media

Improved Matrix Stability

The cross-linked structure provides better physical and chemical resistance than conventional agarose gel filtration media.

High-Temperature Sterilization

TopGF CL-6B can tolerate sterilization at 121°C, supporting processes with strict microbial control requirements.

Gentle Separation

The resin separates molecules without requiring strong adsorption, extreme pH or high-salt elution conditions.

Stable Flow Performance

The 90µm particle size supports convenient slurry preparation, column packing and chromatographic flow.

Broad Macromolecule Compatibility

Suitable for polysaccharides, nucleic acids, viruses, proteins and other biological macromolecules.

TopGF CL-6B vs. Conventional 6B Media

TopGF CL-6B is recommended when improved stability, high-temperature sterilization and reliable process operation are required.

General Operating Procedure

1. Mix the gel slurry gently before use.

2. Remove the storage solution.

3. Wash the resin with purified water or equilibration buffer.

4. Prepare a uniform slurry.

5. Pack the chromatography column at the required bed height.

6. Equilibrate the column with running buffer.

7. Clarify the sample before loading.

8. Apply the sample to the packed column.

9. Elute with the selected buffer.

10. Collect and analyze the required fractions.

Sample volume, column dimensions, bed height and flow rate should be optimized according to the target molecule and required resolution.

Column Packing Recommendations

• Avoid introducing air bubbles during slurry preparation.

• Keep the resin fully suspended before packing.

• Do not allow the packed bed to dry.

• Use filtered and degassed buffers when possible.

• Avoid sudden changes in flow rate.

• Confirm column efficiency before sample purification.

Why Choose SHBC TopGF CL-6B

Controlled Manufacturing

SHBC applies controlled agarose resin manufacturing and quality inspection procedures to support consistent product performance.

Stable Cross-Linked Matrix

The cross-linked structure provides reliable physical stability for chromatography process development.

Flexible Supply

Samples, laboratory packages, pilot quantities and bulk packaging are available.

Custom Services

Packaging volume, storage solution and supply specifications can be adjusted according to project requirements.

Technical Support

SHBC provides support for resin selection, column packing, process optimization and scale-up evaluation.

Custom Packaging and Bulk Supply

Shanghai SanYu Biotechnology Co., Ltd. supplies TopGF CL-6B to universities, research institutes, biotechnology companies, pharmaceutical manufacturers and purification process developers.

Available services include:

• Sample evaluation

• Laboratory packaging

• Pilot-scale supply

• Bulk production

• Custom packaging volume

• OEM and ODM services

• Batch quality documentation

• Column packing guidance

• Purification process support

Please provide the target molecule, sample volume, column dimensions and estimated demand when requesting a quotation.

Frequently Asked Questions

What is TopGF CL-6B?

TopGF CL-6B is a cross-linked agarose gel filtration medium used to separate biological molecules according to size.

What is the particle size?

The average particle size is approximately 90µm.

What samples can be processed?

Typical samples include polysaccharides, DNA, RNA, viruses, proteins, protein complexes and other biological macromolecules.

Can TopGF CL-6B withstand autoclaving?

Yes. The medium can withstand high-temperature sterilization at 121°C under validated conditions.

Does the resin chemically bind the target molecule?

TopGF CL-6B mainly uses a size exclusion mechanism rather than specific chemical binding.

Is it suitable for virus purification?

Yes. It can be evaluated for virus separation, purification, detection and vaccine process development.

Can SHBC provide bulk quantities?

Yes. SHBC supports laboratory samples, pilot-scale orders, custom packaging and bulk production.

Storage and Handling

Store TopGF CL-6B according to the conditions specified in the product documentation.

Do not freeze or allow the gel to dry. Keep the container tightly closed and avoid microbial contamination. Mix gently before use and validate cleaning, sterilization and storage procedures for the final process.

Request a Sample or Quotation

Contact Shanghai SanYu Biotechnology Co., Ltd. for samples, technical documentation, custom packaging or bulk pricing.

Product: TopGF CL-6B
Brand: SHBC
Matrix: Cross-linked agarose
Average Particle Size: 90µm
Sterilization Resistance: Up to 121°C
Main Applications: Polysaccharide, nucleic acid, virus and biomacromolecule purification
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.

For research and industrial purification use.