{"id":1308,"date":"2024-04-25T08:40:26","date_gmt":"2024-04-25T08:40:26","guid":{"rendered":"https:\/\/nanomicronspheres.com\/?post_type=product&p=1308"},"modified":"2025-03-20T09:18:30","modified_gmt":"2025-03-20T09:18:30","slug":"nhs-magnetic-beads","status":"publish","type":"product","link":"https:\/\/nanomicronspheres.com\/es\/products\/nhs-magnetic-beads\/","title":{"rendered":"Cuentas magn\u00e9ticas del NHS"},"content":{"rendered":"

1.NHS Magnetic Beads Overview<\/h2>\n

The functional group on the surface of NHS magnetic beads is NHS, which can couple with proteins or small molecules with primary ammonia to form stable peptide bonds, which can be used for affinity purification of antibodies, antigens, etc. Compared with conventional carboxyl magnetic beads and amino magnetic beads, it have the characteristics of simple operation and mild coupling conditions.<\/p>\n

2. Caracter\u00edsticas del producto<\/h2>\n

Respuesta magn\u00e9tica r\u00e1pida y buena monodispersidad.
\nPeque\u00f1a variaci\u00f3n entre lotes y alta repetibilidad
\nBuena biocompatibilidad y baja citotoxicidad.
\nConveniente para acoplar con una variedad de biomol\u00e9culas y f\u00e1cil de operar.<\/p>\n

3.Product Parameters<\/h2>\n

Tama\u00f1o de part\u00edcula: 0,2 1, 3, 5, 10, 20, 30, 40, 50 \u03bcm
\nConcentraci\u00f3n: 50 mg\/mL
\nSoluci\u00f3n de conservaci\u00f3n: alcohol isoprop\u00edlico
\nLigando: NHS
\nDensidad del ligando: \u226520-30 \u03bcmol\/g
\nCondiciones de almacenamiento: 4 \u2103
\nContenido de hierro (Fe): 30%<\/p>\n

4.How to use<\/h2>\n

Tome una cantidad adecuada de perlas magn\u00e9ticas y l\u00e1velas varias veces con una soluci\u00f3n tamp\u00f3n de pH 8,0. Dispersar una cantidad adecuada de biomol\u00e9culas en un tamp\u00f3n de pH 8,0, redispersar las perlas magn\u00e9ticas en esta soluci\u00f3n y acoplar a 25 \u00b0C durante 3 horas o durante la noche a 4 \u00b0C.<\/p>\n

Despu\u00e9s del acoplamiento, se absorben magn\u00e9ticamente las perlas, se descarta el sobrenadante, se agrega tamp\u00f3n que contiene 1% BSA, se redispersan las perlas y se acoplan a 25 \u00b0C durante 1 hora para bloquear los grupos NHS que no reaccionaron.<\/p>\n

Despu\u00e9s del bloqueo, absorba magn\u00e9ticamente las perlas, retire el sobrenadante, lave las perlas 3 veces con tamp\u00f3n PBS 20 mM pH 7,4, redisperse y almacene a 4 \u00b0 C para su uso posterior. Si es necesario, agregue 0,02% de azida s\u00f3dica para inhibir el crecimiento bacteriano. .<\/p>\n

5.Things to Note<\/h2>\n

Las perlas magn\u00e9ticas tienen una alta densidad y se hundir\u00e1n f\u00e1cilmente si se dejan reposar durante mucho tiempo. Por lo tanto, agite bien antes de usar para obtener una suspensi\u00f3n uniforme de perlas magn\u00e9ticas.<\/p>\n

El tamp\u00f3n de acoplamiento utilizado durante el acoplamiento no debe contener sustancias de amon\u00edaco, de lo contrario afectar\u00e1 la eficiencia del acoplamiento.<\/p>\n

Al bloquear, se puede seleccionar una soluci\u00f3n de etanolamina, una soluci\u00f3n saturada de Tris, etc. para bloquear seg\u00fan las necesidades de la aplicaci\u00f3n. El grupo NHS es muy reactivo y sensible al agua. Por lo tanto, despu\u00e9s de tomar las perlas magn\u00e9ticas, selle las perlas magn\u00e9ticas restantes con una pel\u00edcula selladora y gu\u00e1rdelas a 4\u00b0C.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
N\u00famero de producto<\/td>\nnombre del producto<\/td>\nTama\u00f1o de part\u00edcula<\/td>\n<\/tr>\n
MagNHS200-10<\/td>\nCuentas magn\u00e9ticas del NHS<\/td>\n200nm<\/td>\n<\/tr>\n
MagNHS500-10<\/td>\nCuentas magn\u00e9ticas del NHS<\/td>\n500nm<\/td>\n<\/tr>\n
MagNHS1UM-10<\/td>\nCuentas magn\u00e9ticas del NHS<\/td>\n1 \u03bcm<\/td>\n<\/tr>\n
MagNHS1.5UM-10<\/td>\nCuentas magn\u00e9ticas del NHS<\/td>\n1,5 \u00b5m<\/td>\n<\/tr>\n
MagNHS2.8UM-10<\/td>\nCuentas magn\u00e9ticas del NHS<\/td>\n2,8 \u00b5m<\/td>\n<\/tr>\n
MagNHS3UM-10<\/td>\nCuentas magn\u00e9ticas del NHS<\/td>\n3 \u03bcm<\/td>\n<\/tr>\n
MagNHS5UM-10<\/td>\nCuentas magn\u00e9ticas del NHS<\/td>\n5 \u03bcm<\/td>\n<\/tr>\n
MagNHS10UM-10<\/td>\nNHS Magnetic Bead<\/td>\n10 \u03bcm<\/td>\n<\/tr>\n<\/tbody>\n<\/table>","protected":false},"excerpt":{"rendered":"

El grupo funcional en la superficie de las perlas magn\u00e9ticas de NHS es NHS, que puede acoplarse con prote\u00ednas o mol\u00e9culas peque\u00f1as con amon\u00edaco primario para formar enlaces pept\u00eddicos estables, que pueden usarse para la purificaci\u00f3n por afinidad de anticuerpos, ant\u00edgenos, etc. En comparaci\u00f3n con las perlas magn\u00e9ticas de carboxilo convencionales. y perlas aminomagn\u00e9ticas, las perlas magn\u00e9ticas NHS tienen las caracter\u00edsticas de operaci\u00f3n simple y condiciones de acoplamiento suaves.<\/p>","protected":false},"featured_media":1701,"template":"","meta":{"nf_dc_page":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"product_brand":[],"product_cat":[35,23],"product_tag":[],"class_list":{"0":"post-1308","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-hot-sale-products","7":"product_cat-magnetic-microspheres","8":"desktop-align-left","9":"tablet-align-left","10":"mobile-align-left","12":"first","13":"instock","14":"shipping-taxable","15":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/product\/1308","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/media\/1701"}],"wp:attachment":[{"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/media?parent=1308"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/product_brand?post=1308"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/product_cat?post=1308"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/product_tag?post=1308"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}