![\"Quantum](\"https:\/\/nanomicronspheres.com\/wp-content\/uploads\/2024\/04\/03\u805a\u82ef\u4e59\u70ef\u5fae\u7403\uff08PS\u7403\uff09-300x237.png\")
The fluorescent probe has the characteristics of stable fluorescence signal, excellent colloidal stability, and high biocompatibility, and is especially suitable for the development of fluorescent in vitro diagnostic reagents. . This product can also be used for blood flow measurement, biomarkers, in vivo imaging and calibration of flow cytometers.<\/p>\n
Carboxylation-modified microbeads have a high density of free carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules via water-soluble imine-based reagents such as carbodiimide (EDAC).<\/p>\n
II. Datos f\u00edsicos<\/h2>\n
1. Material: Microesferas de poliestireno recubiertas de puntos cu\u00e1nticos.
\n2. \u00cdndice de refracci\u00f3n: 1,59 a 589 nm, 25 \u2103
\n3. Densidad: 1,05 g\/cm3
\n4. Caracter\u00edsticas: color fluorescente<\/p>\n
III. Instrucciones de an\u00e1lisis<\/h2>\n
1. Contenido de s\u00f3lidos: 1% (10 mg\/ml)
\n2. Tama\u00f1o de part\u00edcula: 100 nm
\n3. Uniformidad: CV<5%
\n4. Longitud de onda de emisi\u00f3n: 610 nm+10 nm
\n5. Longitud de onda de excitaci\u00f3n: <580 nm, se recomienda 365 nm-450 nm
\n6. Medio de dispersi\u00f3n: agua desionizada y trazas de tensioactivo
\n7. Estabilidad: 2 a\u00f1os desde la fecha de f\u00e1brica.<\/p>\n
IV. Use of Quantum Dot Polystyrene Microspheres<\/h2>\n
La superficie de las nanoesferas de puntos cu\u00e1nticos de nuestra empresa (HQD610-10) es rica en grupos funcionales carboxilo, que pueden acoplarse covalentemente con biomol\u00e9culas que contienen amino, como anticuerpos, p\u00e9ptidos, \u00e1cidos nucleicos, etc. Los grupos carboxilo en la superficie de la Las microesferas se encuentran en el activador 1-(Bajo la acci\u00f3n del clorhidrato de 3-dimetilaminopropil)-3-etilcarbodiimida (EDC), reacciona con grupos amino para formar enlaces amida estables, fijando as\u00ed covalentemente biomol\u00e9culas en la superficie de las nanoesferas.<\/p>\n
Precauciones de uso:<\/h3>\n
\u25cb1 Agite y mezcle los QDNB antes de usarlos. Una peque\u00f1a cantidad de precipitaci\u00f3n provocada por un descanso prolongado es normal y no afecta el uso del producto.<\/p>\n
\u25cb2 Para lograr un mejor efecto de acoplamiento, la concentraci\u00f3n de prote\u00edna debe ser de 0,5 a 4 mg\/ml, la concentraci\u00f3n de \u00e1cido nucleico debe ser de 20 a 100 \u03bcM y no debe haber grupos amino libres en el tamp\u00f3n. En particular, no se pueden utilizar tampones Tris, glicina, \u00e1cido ac\u00e9tico y \u00e1cido c\u00edtrico.<\/p>\n
materiales necesitados:<\/h3>\n
\u25cb1 nanoesfera de punto cu\u00e1ntico (10 mg\/ml).<\/p>\n
\u25cb2 Tamp\u00f3n de reacci\u00f3n (para activaci\u00f3n): pH 4-6<\/p>\n
\u25cb3 Activador: EDC\u00b7HCl.<\/p>\n
\u25cb4 Soluci\u00f3n de bloqueo: 1-2% BSA o case\u00edna.<\/p>\n
\u25cb5 Soluci\u00f3n de conservaci\u00f3n (pH 7,0-7,5): \u00e1cido b\u00f3rico o Tris 20-100 mM, NaCl 0,91 TP3T, BSA 0,5-11 TP3T, NaN3 0,091 TP3T.<\/p>\n
Esquema de acoplamiento de referencia<\/h3>\n
\u25cb1 Tome aproximadamente 50 \u00b5l de suspensi\u00f3n de QDNB, disp\u00e9rselos en 450 \u00b5l de tamp\u00f3n de reacci\u00f3n, centrifugue a 12000-15000 rpm durante 10-30 minutos, retire el sobrenadante y disperse los QDNB en 500 \u00b5l de tamp\u00f3n de reacci\u00f3n.<\/p>\n
\u25cb2 Agregue 100-50 \u03bcl de agente de acoplamiento EDC (10 mM), mezcle a temperatura ambiente (25-37 grados) e incube durante 0,5-1 h.<\/p>\n
\u25cb3 Centrifuge at 2000-15000rpm for 10-30min to remove excess coupling agent, etc., then disperse in 500\u03bcl reaction buffer, and disperse with ultrasound;<\/p>\n
\u25cb4 Luego agregue aproximadamente 100 \u03bcg de anticuerpo (la proporci\u00f3n de anticuerpo a microesferas debe optimizarse), mezcle a temperatura ambiente (25-37 grados) e incube durante 0,5-1 h.<\/p>\n
\u25cb51 Centrifugar a 2000-15000 rpm durante 10-30 minutos para eliminar los anticuerpos libres, etc.;<\/p>\n
\u25cb6 Agregue 500 \u03bcl de soluci\u00f3n de bloqueo, disperse con ultrasonido (potencia 10-20%) durante aproximadamente 10 segundos, mezcle a temperatura ambiente (25-37 grados) e incube durante 0,5-1 hora.<\/p>\n
\u25cb7 Centrifugar, retirar la soluci\u00f3n bloqueadora y dispersar en 200 \u03bcl de soluci\u00f3n conservante para su uso posterior.
\nEste protocolo es solo como referencia en las primeras etapas de la investigaci\u00f3n. Se pide a los investigadores que optimicen las condiciones experimentales espec\u00edficas seg\u00fan sus propios proyectos.<\/p>\n
| N\u00famero de producto<\/td>\n | nombre del producto<\/td>\n | Tama\u00f1o de part\u00edcula<\/td>\n<\/tr>\n |
| HQD610-150<\/td>\n | Nanoperlas de puntos cu\u00e1nticos<\/td>\n | 150nm<\/td>\n<\/tr>\n |
| HQD610-10<\/td>\n | Nanoperlas de puntos cu\u00e1nticos<\/td>\n | 100nm<\/td>\n<\/tr>\n<\/tbody>\n<\/table>","protected":false},"excerpt":{"rendered":" Quantum dot polystyrene microspheres (HQD) are prepared by microemulsion polymerization of high-brightness CdSe\/ZnS quantum dots and polystyrene. The surface of the microspheres is modified with carboxyl functional groups to facilitate antibody coupling. Amplifying the fluorescence signal of quantum dots through nanosphere coating can significantly improve the sensitivity of immune detection.<\/p>\n The fluorescent probe has the characteristics of stable fluorescence signal, excellent colloidal stability, and high biocompatibility, and is especially suitable for the development of fluorescent in vitro diagnostic reagents. . This product can also be used for blood flow measurement, biomarkers, in vivo imaging and calibration of flow cytometers.<\/p>","protected":false},"featured_media":1683,"template":"","meta":{"nf_dc_page":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"product_brand":[],"product_cat":[21,35],"product_tag":[],"class_list":{"0":"post-1319","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-fluorescent-microspheres","7":"product_cat-hot-sale-products","8":"desktop-align-left","9":"tablet-align-left","10":"mobile-align-left","12":"first","13":"instock","14":"shipping-taxable","15":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/product\/1319","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/media\/1683"}],"wp:attachment":[{"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/media?parent=1319"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/product_brand?post=1319"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/product_cat?post=1319"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/product_tag?post=1319"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}} |