{"id":1322,"date":"2024-04-25T09:01:12","date_gmt":"2024-04-25T09:01:12","guid":{"rendered":"https:\/\/nanomicronspheres.com\/?post_type=product&p=1322"},"modified":"2025-03-18T09:05:20","modified_gmt":"2025-03-18T09:05:20","slug":"carboxy-magnetic-beads","status":"publish","type":"product","link":"https:\/\/nanomicronspheres.com\/es\/products\/carboxy-magnetic-beads\/","title":{"rendered":"Perlas magn\u00e9ticas carboxi"},"content":{"rendered":"

1.Carboxy Magnetic Beads Overview<\/h2>\n

MagCOOH carboxy magnetic beads use superparamagnetic Fe3O4 as the core and are modified with carboxyl functional groups on the surface. They have the characteristics of high magnetic content and monodispersity. Under the action of specific reagents, the abundant carboxyl functional groups can covalently bind biological ligands such as proteins to the surface of magnetic beads, thereby achieving rapid separation and enrichment of target substances from samples. This is an important issue in molecular biology, An important carrier for research in medicine and other related fields.<\/p>\n

\"Carboxyl
Carboxyl magnetic beads magnetic microspheres<\/figcaption><\/figure>\n

2. Caracter\u00edsticas del producto<\/h2>\n

\uf06cRespuesta magn\u00e9tica r\u00e1pida y tama\u00f1o de part\u00edcula altamente uniforme
\n\uf06cPeque\u00f1a variaci\u00f3n entre lotes y alta repetibilidad
\n\uf06cBuena biocompatibilidad y baja citotoxicidad.
\n\uf06cConveniente para acoplar con una variedad de biomol\u00e9culas y f\u00e1cil de operar<\/p>\n

3. \u00c1mbito de aplicaci\u00f3n<\/h2>\n

\uf06cDrogas dirigidas
\n\uf06cDetecci\u00f3n de sustancias biol\u00f3gicamente activas
\n\uf06cEnriquecimiento y separaci\u00f3n de muestras.<\/p>\n

4. Par\u00e1metros y especificaciones del producto<\/h2>\n

\uf06cTama\u00f1o de part\u00edcula: 200 nm, 1 um, 3 um, 5 um, 10 um, 20 um, 30 um, 50 um
\n\uf06cUniformidad del tama\u00f1o de las part\u00edculas: CV<5%
\n\uf06cConcentraci\u00f3n: 50 mg\/mL
\n\uf06cDisolvente: agua desionizada
\n\uf06cGrupo funcional de superficie: grupo carboxilo (-COOH)
\n\uf06cDensidad de carboxilo: 200 \u03bcmol\/g
\n\uf06cSoluciones aplicables: PBS, Tris-HCl, H2O
\n\uf06cContenido de hierro (Fe): 30%<\/p>\n

5. M\u00e9todo de acoplamiento con biomol\u00e9culas (m\u00e9todo EDC\/NHS)<\/h2>\n

\uf06cActivaci\u00f3n de grupos carboxilo en la superficie de perlas magn\u00e9ticas.
\nDespu\u00e9s de mezclar las perlas magn\u00e9ticas, retire una cantidad adecuada de perlas magn\u00e9ticas en un tubo de centr\u00edfuga, retire magn\u00e9ticamente el sobrenadante y lave las perlas magn\u00e9ticas con el tamp\u00f3n MEST 20 mM preparado (tamp\u00f3n MES 20 mM, pH 5,0, Tween 20 0,051 TP3T) 3 De segunda categor\u00eda. Tome una cantidad adecuada de soluci\u00f3n EDC reci\u00e9n preparada y un volumen igual de soluci\u00f3n NHS para dispersar las perlas magn\u00e9ticas y act\u00edvelas a 25 \u00b0C durante 3 horas.<\/p>\n

\uf06cPerlas magn\u00e9ticas acopladas a biomol\u00e9culas.<\/p>\n

Absorba magn\u00e9ticamente las perlas magn\u00e9ticas, deseche el sobrenadante, disperse una cantidad adecuada de biomol\u00e9culas en un tamp\u00f3n de pH 8,0, redisperse las perlas magn\u00e9ticas en esta soluci\u00f3n y copule a 25 \u00b0C durante 3 horas o 4 \u00b0C durante la noche. Despu\u00e9s del acoplamiento, se absorben magn\u00e9ticamente las perlas, se elimina el sobrenadante, se agrega tamp\u00f3n que contiene 1% BSA, se redispersan las perlas y se acoplan a 25 \u00b0C durante 1 hora para bloquear los grupos carboxilo activados que no han reaccionado.
\nDespu\u00e9s del bloqueo, se absorben magn\u00e9ticamente las perlas, se retira el sobrenadante, se lavan las perlas 3 veces con tamp\u00f3n PBS 20 mM de pH 7,4, se redispersan y se almacenan a 4 \u00b0C. Si es necesario, agregue 0,02% de azida s\u00f3dica para inhibir el crecimiento bacteriano.<\/p>\n

6.things to note<\/h2>\n

\uf06cLas cuentas magn\u00e9ticas tienen una alta densidad y se hundir\u00e1n f\u00e1cilmente si se dejan en reposo durante mucho tiempo. Por lo tanto, agite bien antes de usar para obtener una suspensi\u00f3n uniforme de perlas magn\u00e9ticas.<\/p>\n

7. Condiciones de almacenamiento y fecha de caducidad<\/h2>\n

\uf06cCondiciones de almacenamiento: Almacenar a 2 ~ 8 \u2103, no congelar ni secar.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
N\u00famero de producto<\/td>\nnombre del producto<\/td>\nTama\u00f1o de part\u00edcula<\/td>\n<\/tr>\n
MagCOOH100-10<\/td>\nPerlas magn\u00e9ticas carboxi<\/td>\n100nm<\/td>\n<\/tr>\n
MagCOOH200-10<\/td>\nPerlas magn\u00e9ticas carboxi<\/td>\n200nm<\/td>\n<\/tr>\n
MagCOOH500-10<\/td>\nPerlas magn\u00e9ticas carboxi<\/td>\n500nm<\/td>\n<\/tr>\n
MagCOOH1UM-10<\/td>\nPerlas magn\u00e9ticas carboxi<\/td>\n1 \u03bcm<\/td>\n<\/tr>\n
MagCOOH2UM-10<\/td>\nPerlas magn\u00e9ticas carboxi<\/td>\n2 \u03bcm<\/td>\n<\/tr>\n
MagCOOH3UM-10<\/td>\nPerlas magn\u00e9ticas carboxi<\/td>\n3 \u03bcm<\/td>\n<\/tr>\n
MagCOOH5UM-10<\/td>\nPerlas magn\u00e9ticas carboxi<\/td>\n5 \u03bcm<\/td>\n<\/tr>\n
MagCOOH10UM-10<\/td>\nPerlas magn\u00e9ticas carboxi<\/td>\n10 \u03bcm<\/td>\n<\/tr>\n
MagCOOH20UM-10<\/td>\nPerlas magn\u00e9ticas carboxi<\/td>\n20\u03bcm<\/td>\n<\/tr>\n
MagCOOH30UM-10<\/td>\nPerlas magn\u00e9ticas carboxi<\/td>\n30\u03bcm<\/td>\n<\/tr>\n
MagCOOH50UM-10<\/td>\nPerlas magn\u00e9ticas carboxi<\/td>\n50 \u03bcm<\/td>\n<\/tr>\n
MagCOOH100UM-10<\/td>\nPerlas magn\u00e9ticas carboxi<\/td>\n100 \u03bcm<\/td>\n<\/tr>\n<\/tbody>\n<\/table>","protected":false},"excerpt":{"rendered":"

Las perlas magn\u00e9ticas carboxilo MagCOOH utilizan Fe3O4 superparamagn\u00e9tico como n\u00facleo y est\u00e1n modificadas con grupos funcionales carboxilo en la superficie. Tienen las caracter\u00edsticas de alto contenido magn\u00e9tico y monodispersidad. Bajo la acci\u00f3n de reactivos espec\u00edficos, los abundantes grupos funcionales carboxilo pueden unir covalentemente ligandos biol\u00f3gicos como prote\u00ednas a la superficie de perlas magn\u00e9ticas, logrando as\u00ed una r\u00e1pida separaci\u00f3n y enriquecimiento de las sustancias objetivo de las muestras. Este es un tema importante en biolog\u00eda molecular, un importante camino para la investigaci\u00f3n en medicina y otros campos relacionados.<\/p>","protected":false},"featured_media":1697,"template":"","meta":{"nf_dc_page":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"product_brand":[],"product_cat":[35,23],"product_tag":[],"class_list":{"0":"post-1322","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-hot-sale-products","7":"product_cat-magnetic-microspheres","8":"desktop-align-left","9":"tablet-align-left","10":"mobile-align-left","12":"first","13":"instock","14":"shipping-taxable","15":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/product\/1322","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/media\/1697"}],"wp:attachment":[{"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/media?parent=1322"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/product_brand?post=1322"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/product_cat?post=1322"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/product_tag?post=1322"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}