{"id":1325,"date":"2024-04-25T09:07:25","date_gmt":"2024-04-25T09:07:25","guid":{"rendered":"https:\/\/nanomicronspheres.com\/?post_type=product&p=1325"},"modified":"2025-03-18T08:28:49","modified_gmt":"2025-03-18T08:28:49","slug":"epoxy-magnetic-beads","status":"publish","type":"product","link":"https:\/\/nanomicronspheres.com\/es\/products\/epoxy-magnetic-beads\/","title":{"rendered":"Cuentas magn\u00e9ticas epoxi"},"content":{"rendered":"

1. Descripci\u00f3n general de las perlas magn\u00e9ticas de epoxy<\/h2>\n

Las perlas magn\u00e9ticas epoxi tienen un grupo epoxi como grupo funcional en la superficie, que puede acoplarse con prote\u00ednas o mol\u00e9culas peque\u00f1as con aminas primarias, y puede usarse para la purificaci\u00f3n por afinidad de anticuerpos, ant\u00edgenos y otros aspectos. En comparaci\u00f3n con las perlas magn\u00e9ticas carbox\u00edlicas y amino convencionales, las perlas magn\u00e9ticas epoxi se caracterizan por un funcionamiento sencillo y condiciones de acoplamiento suaves.<\/p>\n

2. Caracter\u00edsticas de las perlas magn\u00e9ticas de epoxi<\/h2>\n

\uf06c Acoplamiento r\u00e1pido y sencillo en un solo paso
\n\uf06c Baja variaci\u00f3n entre lotes y alta reproducibilidad
\n\uf06c La densidad moderada del grupo epoxi y la morfolog\u00eda superficial uniforme maximizan la tasa de acoplamiento.<\/p>\n

3. Par\u00e1metros del producto<\/h2>\n

\uf06c Tama\u00f1o de part\u00edcula: 1\u03bcm, 3\u03bcm, 5\u03bcm
\n\uf06c Concentraci\u00f3n: 50 mg\/mL
\n\uf06c Soluci\u00f3n de almacenamiento: alcohol isoprop\u00edlico
\n\uf06c Grupo de formulaci\u00f3n: epoxi
\n\uf06c Densidad del ligando: \u2265250 \u03bcmol\/g<\/p>\n

4.M\u00e9todo de uso<\/h2>\n

Tome una cantidad adecuada de perlas de epoxi y l\u00e1velas varias veces con una soluci\u00f3n tamp\u00f3n de pH 8.0. Disperse la cantidad adecuada de biomol\u00e9culas.<\/p>\n

Dispersar la cantidad adecuada de biomol\u00e9culas en tamp\u00f3n de pH 8,0 y volver a dispersar las microesferas en esta soluci\u00f3n y acoplarlas a 25 \u2103 durante 6 horas o a 4 \u2103 durante la noche.<\/p>\n

Las microesferas se acoplaron durante 6 horas a 25\u00b0C o durante la noche a 4\u00b0C. Al final del acoplamiento, el sobrenadante se aspira magn\u00e9ticamente, se descarta y las microesferas se redispersan a\u00f1adiendo tamp\u00f3n que contiene etanolamina 20 mM y se acoplan durante 3 horas a 25\u00b0C para sellar los grupos epoxi que no han reaccionado. Despu\u00e9s del sellado, succi\u00f3n magn\u00e9tica, retire el sobrenadante, lave las microesferas con tamp\u00f3n PBS 20 mM de pH 7,4 tres veces y vuelva a dispersarlas, gu\u00e1rdelas a 4 \u2103 como reserva; si es necesario, agregue azida s\u00f3dica 0,02% para inhibir el crecimiento de bacterias. .<\/p>\n

5. Precauciones<\/h2>\n

\uf06c Agitar bien antes de usar para obtener una suspensi\u00f3n homog\u00e9nea de microesferas.<\/p>\n

\uf06c El tamp\u00f3n de acoplamiento utilizado para el acoplamiento no debe contener amon\u00edaco, de lo contrario la eficiencia del acoplamiento se ver\u00e1 afectada.<\/p>\n

\uf06c Al sellar, seleccione la soluci\u00f3n tamp\u00f3n adecuada para sellar seg\u00fan las necesidades de la aplicaci\u00f3n.<\/p>\n

\uf06c Almacenar a 4 \u2103 durante 3 a\u00f1os.<\/p>\n\n\n\n\n\n\n\n\n\n\n
N\u00famero de producto<\/td>\nnombre del producto<\/td>\nTama\u00f1o de part\u00edcula<\/td>\n<\/tr>\n
MagEP1UM-10<\/td>\nCuentas magn\u00e9ticas epoxi<\/td>\n1 \u03bcm<\/td>\n<\/tr>\n
MagEP2UM-10<\/td>\nCuentas magn\u00e9ticas epoxi<\/td>\n2 \u03bcm<\/td>\n<\/tr>\n
MagEP3UM-10<\/td>\nCuentas magn\u00e9ticas epoxi<\/td>\n2 \u03bcm<\/td>\n<\/tr>\n
MagEP5UM-10<\/td>\nCuentas magn\u00e9ticas epoxi<\/td>\n2 \u03bcm<\/td>\n<\/tr>\n
MagEP10UM-10<\/td>\nCuentas magn\u00e9ticas epoxi<\/td>\n10 \u03bcm<\/td>\n<\/tr>\n
MagEP15UM-10<\/td>\nCuentas magn\u00e9ticas epoxi<\/td>\n15\u03bcm<\/td>\n<\/tr>\n
MagEP20UM-10<\/td>\nCuentas magn\u00e9ticas epoxi<\/td>\n20\u03bcm<\/td>\n<\/tr>\n<\/tbody>\n<\/table>","protected":false},"excerpt":{"rendered":"

Las perlas magn\u00e9ticas epoxi tienen un grupo epoxi como grupo funcional en la superficie, que puede acoplarse con prote\u00ednas o mol\u00e9culas peque\u00f1as con aminas primarias, y puede usarse para la purificaci\u00f3n por afinidad de anticuerpos, ant\u00edgenos y otros aspectos. En comparaci\u00f3n con las perlas magn\u00e9ticas carbox\u00edlicas y amino convencionales, las perlas magn\u00e9ticas epoxi se caracterizan por un funcionamiento sencillo y condiciones de acoplamiento suaves.<\/p>","protected":false},"featured_media":1699,"template":"","meta":{"nf_dc_page":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"product_brand":[],"product_cat":[35,23],"product_tag":[],"class_list":{"0":"post-1325","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-hot-sale-products","7":"product_cat-magnetic-microspheres","8":"desktop-align-left","9":"tablet-align-left","10":"mobile-align-left","12":"first","13":"instock","14":"shipping-taxable","15":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/product\/1325","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/media\/1699"}],"wp:attachment":[{"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/media?parent=1325"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/product_brand?post=1325"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/product_cat?post=1325"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/nanomicronspheres.com\/es\/wp-json\/wp\/v2\/product_tag?post=1325"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}