Product Overview
SHBC HardHIC Phenyl (LS) is a high-rigidity agarose-based hydrophobic interaction chromatography (HIC) resin designed for the separation and purification of proteins and other biomolecules.
The resin combines a high-rigidity agarose matrix with a Phenyl hydrophobic ligand. The rigid agarose matrix provides low backpressure and high flow capability, making the HardHIC series suitable for large-scale biomolecule separation and purification.
HardHIC Phenyl (LS) provides a reference dynamic binding capacity of approximately 15 mg/mL for BSA.
Key Features
High-rigidity agarose matrix
Phenyl hydrophobic ligand
LS specification
≈15 mg/mL dynamic binding capacity for BSA
Low backpressure
High flow capability
Suitable for protein purification
Suitable for biomolecule separation
Designed for large-scale purification
Package sizes from 25 mL to 20 L
Technical Specifications
Parameter | Specification |
|---|
Product Name | HardHIC Phenyl (LS) |
Brand | SHBC |
Product Type | High Rigidity Agarose HIC Resin |
Matrix | High Rigidity Agarose |
Ligand | Phenyl |
Specification | LS |
Chromatography Mode | Hydrophobic Interaction Chromatography |
Dynamic Binding Capacity | ≈15 mg/mL (BSA) |
Main Characteristics | Low Backpressure / High Flow |
Package | 25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L |
SHBC Art.No. | Packaging Specification |
|---|
A3064 | 25 mL |
A3065 | 100 mL |
A3066 | 300 mL |
A3067 | 1 L |
A3068 | 5 L |
A3069 | 10 L |
A3070 | 20 L |
High Rigidity Agarose Matrix
HardHIC Phenyl (LS) uses a high-rigidity agarose matrix designed for efficient chromatographic processing.
According to the supplied product information, the HardHIC matrix provides:
These characteristics make the resin suitable for downstream purification processes where processing efficiency and scalability are important.
Phenyl Hydrophobic Ligand
HardHIC Phenyl (LS) uses a Phenyl hydrophobic ligand.
The Phenyl ligand provides hydrophobic interaction with proteins and other biomolecules, allowing separation based on differences in hydrophobic properties.
Combined with the high-rigidity agarose matrix, the resin provides hydrophobic selectivity together with efficient chromatographic processing.
Dynamic Binding Capacity
The specified reference dynamic binding capacity is:
≈15 mg/mL (BSA)
Bovine Serum Albumin (BSA) is used as the reference protein for the dynamic binding capacity listed in the supplied product table.
Actual binding performance can vary depending on the target biomolecule and chromatographic conditions.
Low Backpressure and High Flow
The HardHIC series is characterized by its high-rigidity agarose matrix.
The rigid matrix is designed to provide low backpressure and high flow capability, supporting efficient chromatographic processing.
These characteristics are particularly useful when scaling purification processes to larger sample and column volumes.
Hydrophobic Interaction Chromatography
Hydrophobic interaction chromatography separates proteins and other biomolecules according to differences in their hydrophobic properties.
HardHIC Phenyl (LS) provides a Phenyl-functionalized stationary phase for hydrophobic interaction-based separation.
The media can be incorporated into downstream purification workflows for proteins and other biomolecules.
Applications
SHBC HardHIC Phenyl (LS) can be used for:
Protein purification
Recombinant protein purification
Biomolecule separation
Hydrophobic interaction chromatography
Large-scale biomolecule purification
Biopharmaceutical downstream processing
Process chromatography
Separation based on hydrophobic properties
Chromatography process development
Scale-up purification
Product Advantages
HardHIC Phenyl (LS) combines:
High-rigidity agarose matrix
Phenyl hydrophobic selectivity
≈15 mg/mL BSA binding capacity
Low backpressure
High flow capability
Suitable for large-scale separation
Suitable for large-scale purification
Multiple packaging options up to 20 L
HardHIC Phenyl LS vs. HS
HardHIC Phenyl is available in LS and HS specifications in the supplied product table.
Feature | HardHIC Phenyl (LS) | HardHIC Phenyl (HS) |
|---|
Matrix | High Rigidity Agarose | High Rigidity Agarose |
Ligand | Phenyl | Phenyl |
Specification | LS | HS |
Reference Protein | BSA | BSA |
Dynamic Binding Capacity | ≈15 mg/mL | ≈27 mg/mL |
Main Characteristics | Low Backpressure / High Flow | Low Backpressure / High Flow |
The supplied information distinguishes the two products primarily by their LS/HS designation and reference BSA dynamic binding capacity.
The supplied table does not separately define the technical meaning of LS and HS, so these designations are retained without adding an unsupported interpretation.
Designed for Large-Scale Purification
HardHIC Phenyl (LS) belongs to the high-rigidity agarose HIC series designed for large-scale separation and purification of biomolecules.
The combination of low backpressure and high flow capability supports efficient chromatographic processing across different purification scales.
Available Package Sizes
HardHIC Phenyl (LS) is available in:
25 mL
100 mL
300 mL
1 L
5 L
10 L
20 L
This package range supports laboratory evaluation, process development and larger-scale purification.
Frequently Asked Questions
What is HardHIC Phenyl (LS)?
HardHIC Phenyl (LS) is a high-rigidity agarose-based hydrophobic interaction chromatography resin using a Phenyl ligand.
What matrix does HardHIC Phenyl (LS) use?
It uses a high-rigidity agarose matrix.
What ligand is used?
The hydrophobic ligand is Phenyl.
What is the dynamic binding capacity?
The specified reference dynamic binding capacity is approximately 15 mg/mL for BSA.
What does LS mean?
The supplied product table identifies this resin as HardHIC Phenyl (LS) but does not provide a separate definition of LS. Therefore, LS is retained as the product specification designation.
What is the difference between HardHIC Phenyl LS and HS?
According to the supplied table, the LS version has approximately 15 mg/mL BSA dynamic binding capacity, while the HS version has approximately 27 mg/mL BSA dynamic binding capacity.
What are the main characteristics of the HardHIC series?
The supplied information highlights high-rigidity agarose, low backpressure and high flow rates.
Is HardHIC Phenyl (LS) suitable for large-scale purification?
Yes. The HardHIC series is described as suitable for large-scale separation and purification of biomolecules.
What applications is this resin suitable for?
Typical applications include protein purification, biomolecule separation, hydrophobic interaction chromatography and large-scale downstream purification.
What package sizes are available?
Available packages are 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L.
What are the SHBC Art.No. numbers?
The corresponding SHBC Art.No. are A3064, A3065, A3066, A3067, A3068, A3069 and A3070.
What is the English product name?
The supplied English name is High Rigidity Agarose Phenyl HIC Resin LS.
What brand is HardHIC Phenyl (LS)?
HardHIC Phenyl (LS) is supplied under the SHBC brand.
