Product Overview
HEPES (4-(2-Hydroxyethyl)-1-piperazineethanesulfonic Acid) is one of the most widely used Good's Buffers in life science research. It provides excellent buffering capacity near physiological pH while exhibiting low toxicity, minimal metal ion binding and outstanding chemical stability.
Unlike bicarbonate buffer systems, HEPES maintains a stable pH without requiring a CO₂ atmosphere, making it an ideal choice for cell culture media, protein purification, enzyme assays, diagnostic reagent formulation and molecular biology applications.
Key Features
High purity pharmaceutical grade
Excellent buffering capacity
Stable physiological pH
Highly water soluble
Low metal ion binding
Low biological interference
Excellent chemical stability
Suitable for cell culture
Reliable batch-to-batch consistency
Ideal for life science research
Technical Specifications
Parameter | Specification |
|---|
Product Code | RH0011 |
Product Name | HEPES Buffer |
Chemical Name | 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic Acid |
CAS No. | 7365-45-9 |
Molecular Formula | C8H18N2O4S |
Molecular Weight | 238.30 g/mol |
Purity | Pharmaceutical Grade |
pKa (25°C) | 7.55 |
Effective Buffer Range | pH 6.8–8.2 |
Appearance | White Crystalline Powder |
Solubility | Soluble in Water |
Package | 500 g / 1 kg |
Applications
Cell Culture Media
Molecular Biology
Protein Purification
Enzyme Assays
Diagnostic Reagent Development
Immunoassays
Biochemistry Research
Pharmaceutical Research
Laboratory Buffer Preparation
Biotechnology Applications
Product Advantages
RH0011 HEPES Buffer provides excellent buffering performance under physiological conditions and is one of the preferred buffering agents for cell culture and molecular biology research. Its ability to maintain stable pH without CO₂ makes it especially suitable for open culture systems and routine laboratory experiments.
Manufactured under strict quality standards, SANYU GROUP HEPES Buffer delivers high purity, excellent consistency and reliable performance for research laboratories, biotechnology companies, diagnostic manufacturers and pharmaceutical industries worldwide.
Storage and Handling
Store in a cool, dry and well-ventilated place.
Keep the container tightly closed after use.
Protect from moisture and direct sunlight.
Store at room temperature.
Avoid contamination during handling.
Packaging
Standard packaging:
OEM packaging and bulk quantities are available upon request.
Frequently Asked Questions (FAQ)
What is HEPES Buffer used for?
HEPES Buffer is widely used in cell culture, molecular biology, protein purification, enzyme assays, immunoassays and diagnostic reagent development.
What is the effective pH range of HEPES Buffer?
HEPES Buffer provides effective buffering between pH 6.8 and 8.2, with a pKa of approximately 7.55 at 25°C.
Why is HEPES commonly used in cell culture?
HEPES maintains a stable physiological pH without requiring a CO₂ atmosphere, making it ideal for cell culture and biological experiments performed outside CO₂ incubators.
How should HEPES Buffer be stored?
Store the product in a cool, dry place away from moisture and direct sunlight. Keep the container tightly sealed after opening.
Do you provide OEM packaging?
Yes. SANYU GROUP provides OEM labeling, customized packaging and bulk supply services for distributors, research laboratories, biotechnology companies and diagnostic reagent manufacturers.
