Product Overview
SHBC SP TopIEX XL is a high-loading strong cation exchange chromatography media designed for high-capacity biomolecule purification.
The XL series uses a highly crosslinked agarose matrix modified with linear dextran chains. Functional groups are subsequently introduced onto the dextran surface extenders.
This structure helps reduce steric hindrance between biomolecules and increases the accessible binding area, resulting in significantly improved target molecule loading capacity.
SP TopIEX XL uses SP (sulfopropyl) functional groups and acts as a strong cation exchanger.
Key Features
High-loading ion exchange chromatography media
Strong cation exchange functionality
Highly crosslinked agarose matrix
Linear dextran surface extenders
SP (sulfopropyl) ligand
≥160 mg/mL dynamic binding capacity for lysozyme
Recommended flow rate: 60–300 cm/h
Pressure resistance: 0.3 MPa
Working pH range: 4–13
CIP pH range: 3–14
Reduced steric hindrance
High accessible binding capacity
Suitable for process-scale protein purification
Technical Specifications
Parameter | Specification |
|---|
Product Name | SP TopIEX XL |
Brand | SHBC |
Product Type | High Loading Strong Cation Exchange Chromatography Media |
Matrix | Highly Crosslinked Agarose with Linear Dextran |
Ligand | SP (Sulfopropyl) |
Ion Exchange Type | Strong Cation Exchanger |
Dynamic Binding Capacity | ≥160 mg/mL (Lysozyme) |
Recommended Flow Rate | 60–300 cm/h |
Pressure Resistance | 0.3 MPa |
Working pH Range | 4–13 |
CIP pH Range | 3–14 |
Package | 25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L |
SHBC Art.No. | Packaging Specification |
A2075 | 25 mL |
A2076 | 100 mL |
A2077 | 300 mL |
A2078 | 1 L |
A2079 | 5 L |
A2080 | 10 L |
A2081 | 20 L |
High Loading XL Matrix Design
The key feature of SP TopIEX XL is its XL high-loading structure.
Linear dextran molecules are coupled onto a highly crosslinked agarose matrix, and the functional groups are then introduced onto the dextran chains.
This extended surface architecture reduces steric hindrance between target biomolecules and increases the effective binding area available within the chromatography media.
As a result, the XL structure is designed to provide substantially higher target molecule loading than conventional agarose ion exchange media.
Strong Cation Exchange Chromatography
SP TopIEX XL uses SP (sulfopropyl) as the ion exchange ligand and functions as a strong cation exchanger.
Under appropriate pH and buffer conditions, positively charged biomolecules interact with the negatively charged SP functional groups.
After sample loading and washing, bound target molecules can be eluted by changing ionic strength or other chromatographic conditions.
High Dynamic Binding Capacity
SP TopIEX XL provides a specified dynamic binding capacity of:
≥160 mg/mL for lysozyme
The high binding capacity is one of the main advantages of the XL series and can help reduce the required chromatography bed volume for high-load purification processes.
High-Capacity Purification
Higher binding capacity can provide several process advantages:
Increased target loading per unit volume of media
Reduced chromatography bed volume
Improved process productivity
Potential reduction in buffer consumption
More efficient processing of high-concentration feed streams
Improved suitability for scale-up and process chromatography
Recommended Flow Rate and Pressure
SP TopIEX XL supports a recommended linear flow rate of:
60–300 cm/h
The specified pressure resistance is:
0.3 MPa
These operating characteristics make the media suitable for laboratory development as well as process-scale chromatography workflows.
Wide pH Stability
SP TopIEX XL provides broad pH tolerance:
Working pH: 4–13
CIP pH: 3–14
The broad pH range provides flexibility for process development, elution optimization and cleaning-in-place procedures.
Applications
SHBC SP TopIEX XL can be used for:
Lysozyme purification
Recombinant protein purification
Protein purification
Biomolecule separation
High-capacity ion exchange chromatography
Capture purification
Intermediate purification
High-concentration feed processing
Process-scale biopharmaceutical purification
Downstream purification process development
Product Advantages
SP TopIEX XL combines:
High dynamic binding capacity
Strong SP cation exchange functionality
Highly crosslinked agarose matrix
Linear dextran surface extenders
Reduced steric hindrance
Increased accessible binding area
Broad pH stability
Process-compatible flow rates
High-capacity biomolecule purification
The main difference between XL media and conventional ion exchange media is the surface-extender architecture.
Feature | SP TopIEX XL | Conventional Ion Exchange Media |
Matrix | Highly crosslinked agarose + linear dextran | Agarose matrix |
Surface Extender | Yes | Typically no |
Ligand | SP | SP or other ion exchange ligands |
Main Positioning | High Loading | General ion exchange purification |
Binding Capacity | ≥160 mg/mL Lysozyme | Product-dependent |
Main Advantage | Reduced steric hindrance and higher loading | General-purpose separation |
SP TopIEX XL is particularly suitable when high binding capacity and reduced chromatography bed volume are important process considerations.
Storage and Handling
Keep the chromatography media in its original container and protect it from contamination during storage and handling.
Before use, equilibrate the packed bed with an appropriate buffer according to the purification process.
Sample conditions, buffer composition, loading amount, flow rate, elution conditions and CIP procedures should be optimized according to the target biomolecule and process requirements.
Frequently Asked Questions
What is SP TopIEX XL?
SP TopIEX XL is a high-loading strong cation exchange chromatography media designed for high-capacity protein and biomolecule purification.
What ligand is used?
The functional ligand is SP (sulfopropyl).
Is SP TopIEX XL a strong or weak cation exchanger?
It is a strong cation exchange chromatography media.
What is the binding capacity?
The specified dynamic binding capacity is ≥160 mg/mL for lysozyme.
Why does the XL series have high binding capacity?
The XL structure uses linear dextran surface extenders on a highly crosslinked agarose matrix, helping reduce steric hindrance and increase the accessible binding area.
What is the recommended flow rate?
The recommended flow rate is 60–300 cm/h.
What is the pressure resistance?
The specified pressure resistance is 0.3 MPa.
What is the working pH range?
The working pH range is 4–13.
What is the CIP pH range?
The CIP pH range is 3–14.
Is SP TopIEX XL suitable for lysozyme purification?
Yes. The supplied specification uses lysozyme as the reference protein for its dynamic binding capacity, with a specified capacity of ≥160 mg/mL.
What package sizes are available?
SHBC offers 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L packages.
What are the SHBC Art.No. numbers?
The corresponding SHBC Art.No. are A2075, A2076, A2077, A2078, A2079, A2080 and A2081.
What brand is SP TopIEX XL?
SP TopIEX XL is supplied under the SHBC brand.
