Product Overview
SHBC HardIEX S is a high-rigidity strong cation exchange chromatography media designed for efficient protein and biomolecule purification.
The media uses a chemically modified high-rigidity agarose matrix engineered for improved mechanical performance. Compared with conventional fast-flow agarose ion exchange media, the HardIEX series is designed to provide higher flow rates, lower back pressure and high binding capacity.
HardIEX S uses an SP (sulfopropyl) functional group and acts as a strong cation exchanger.
Key Features
High-rigidity agarose matrix
Strong cation exchange chromatography media
SP (sulfopropyl) ligand
Approximately 90 μm average particle size
≥30 mg/mL dynamic binding capacity for BSA
Recommended flow rate: 90–500 cm/h
Pressure resistance: 0.5 MPa / 5 bar
Working pH range: 4–13
CIP pH range: 3–14
Fast flow characteristics
Low back pressure
High mechanical stability
Suitable for process-scale protein purification
Technical Specifications
Parameter | Specification |
|---|
Product Name | HardIEX S |
Brand | SHBC |
Product Type | High-Rigidity Strong Cation Exchange Chromatography Media |
Matrix | High-Rigidity Agarose |
Ligand | SP (Sulfopropyl) |
Ion Exchange Type | Strong Cation Exchanger |
Average Particle Size | Approx. 90 μm |
Dynamic Binding Capacity | ≥30 mg/mL (BSA) |
Recommended Flow Rate | 90–500 cm/h |
Pressure Resistance | 0.5 MPa / 5 bar |
Working pH Range | 4–13 |
CIP pH Range | 3–14 |
Package | 25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L |
SHBC Art.No. | Packaging Specification |
A2089 | 25 mL |
A2090 | 100 mL |
A2091 | 300 mL |
A2092 | 1 L |
A2093 | 5 L |
A2094 | 10 L |
A2095 | 20 L |
High-Rigidity Agarose Matrix
HardIEX S uses a high-rigidity agarose matrix produced by chemical modification and reinforcement of a conventional agarose framework.
This matrix is designed to provide improved mechanical strength and can tolerate pressures up to 5 bar (0.5 MPa).
Compared with conventional fast-flow agarose ion exchange media, the HardIEX platform is designed for:
The supplier specifically positions this high-rigidity matrix for fast-flow and high-load purification applications.
Strong Cation Exchange Chromatography
HardIEX S uses SP (sulfopropyl) as the ion-exchange ligand.
SP is a strong cation exchange functional group that interacts with positively charged biomolecules under suitable buffer and pH conditions.
After sample loading, unbound impurities can be washed away, while bound target molecules can be eluted by changing ionic strength or other chromatographic conditions.
High Dynamic Binding Capacity
HardIEX S provides a specified dynamic binding capacity of:
≥30 mg/mL for BSA
This capacity, combined with the high-rigidity matrix, provides a practical balance between protein loading and high-flow processing.
High Flow Rate and Low Back Pressure
One of the major advantages of HardIEX S is its high-flow capability.
The recommended linear flow rate is:
90–500 cm/h
The high-rigidity matrix helps maintain bed stability under elevated flow conditions while reducing excessive back pressure.
This can help shorten chromatography cycle times and improve process productivity.
Pressure Resistance up to 5 Bar
HardIEX S provides a specified pressure resistance of:
0.5 MPa / 5 bar
The improved mechanical strength makes the media suitable for chromatography processes requiring higher flow rates and more robust column operation.
Wide pH Stability
HardIEX S provides broad pH stability:
Working pH: 4–13
CIP pH: 3–14
The broad pH tolerance provides flexibility for purification process development, elution optimization and cleaning-in-place procedures.
Applications
SHBC HardIEX S can be used for:
Protein purification
BSA purification
Recombinant protein purification
Biomolecule separation
Strong cation exchange chromatography
Capture purification
Intermediate purification
High-flow downstream processing
Process-scale biopharmaceutical purification
Chromatography process development and scale-up
Product Advantages
HardIEX S combines:
High-rigidity agarose matrix
Strong SP cation exchange functionality
≥30 mg/mL BSA binding capacity
High recommended flow rate
Low back pressure
Pressure resistance up to 5 bar
Broad pH stability
Good mechanical performance
Suitable for scalable downstream purification
HardIEX S is designed for applications where improved mechanical performance and higher processing speed are important.
Feature | HardIEX S | Conventional Fast Flow Media |
Matrix | High-Rigidity Agarose | Crosslinked Agarose |
Particle Size | Approx. 90 μm | Approx. 90 μm |
Ligand | SP | SP |
Pressure Resistance | 0.5 MPa | Typically lower |
Flow Capability | Up to 500 cm/h recommended | Lower depending on media |
Main Advantage | High flow, low back pressure, high rigidity | General-purpose purification |
The particle sizes are similar, but the high-rigidity matrix provides improved mechanical performance for demanding chromatography processes.
Storage and Handling
Keep the chromatography media in its original container and protect it from contamination during storage and handling.
Before use, equilibrate the packed bed with a suitable buffer according to the purification process.
Sample conditions, buffer composition, loading amount, flow rate, elution conditions and CIP procedures should be optimized according to the target biomolecule and process requirements.
Frequently Asked Questions
What is HardIEX S?
HardIEX S is a high-rigidity strong cation exchange chromatography media designed for high-flow protein and biomolecule purification.
What matrix is used?
The media uses a high-rigidity agarose matrix.
What ligand is used?
The functional ligand is SP (sulfopropyl).
Is HardIEX S a strong or weak cation exchanger?
It is a strong cation exchange chromatography media.
What is the average particle size?
The HardIEX platform has an average particle size of approximately 90 μm.
What is the dynamic binding capacity?
The specified dynamic binding capacity is ≥30 mg/mL for BSA.
What is the recommended flow rate?
The recommended flow rate is 90–500 cm/h.
What is the pressure resistance?
HardIEX S can tolerate up to 0.5 MPa / 5 bar.
What is the working pH range?
The working pH range is 4–13.
What is the CIP pH range?
The CIP pH range is 3–14.
What are the main advantages of HardIEX S?
Its key advantages include high mechanical rigidity, fast flow rate, low back pressure, strong cation exchange functionality and high binding capacity.
What package sizes are available?
SHBC offers 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L packages.
What are the SHBC Art.No. numbers?
The corresponding SHBC Art.No. are A2089, A2090, A2091, A2092, A2093, A2094 and A2095.
What brand is HardIEX S?
HardIEX S is supplied under the SHBC brand.
