Product Overview
SHBC HardIEX DEAE is a high-rigidity weak anion exchange chromatography media designed for protein and biomolecule purification.
The product uses a high-rigidity agarose matrix engineered for improved mechanical performance, fast flow and low back pressure.
HardIEX DEAE uses DEAE (diethylaminoethyl) functional groups and acts as a weak anion exchanger, providing charge-based separation of negatively charged biomolecules under suitable buffer and pH conditions.
Key Features
High-rigidity agarose matrix
Weak anion exchange chromatography media
DEAE functional ligand
Approximately 90 μm average particle size
≥90 mg/mL dynamic binding capacity for ovalbumin
Recommended flow rate: 90–500 cm/h
Pressure resistance: 0.5 MPa / 5 bar
Working pH range: 2–9
Long-term pH stability: 2–12
CIP pH range: 2–14
Fast flow characteristics
Low back pressure
High mechanical stability
Suitable for scalable biomolecule purification
Technical Specifications
Parameter | Specification |
|---|
Product Name | HardIEX DEAE |
Brand | SHBC |
Product Type | High-Rigidity Weak Anion Exchange Chromatography Media |
Matrix | High-Rigidity Agarose |
Ligand | DEAE (Diethylaminoethyl) |
Ion Exchange Type | Weak Anion Exchanger |
Average Particle Size | Approx. 90 μm |
Dynamic Binding Capacity | ≥90 mg/mL (Ovalbumin) |
Recommended Flow Rate | 90–500 cm/h |
Pressure Resistance | 0.5 MPa / 5 bar |
Working pH Range | 2–9 |
Long-Term pH Range | 2–12 |
CIP pH Range | 2–14 |
Package | 25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L |
SHBC Art.No. | Packaging Specification |
A2103 | 25 mL |
A2104 | 100 mL |
A2105 | 300 mL |
A2106 | 1 L |
A2107 | 5 L |
A2108 | 10 L |
A2109 | 20 L |
High-Rigidity Agarose Matrix
HardIEX DEAE uses a high-rigidity agarose matrix developed by chemically modifying and reinforcing a conventional agarose framework.
The matrix is designed to provide:
Improved mechanical strength
Fast flow rates
Low back pressure
Stable packed-bed performance
Suitability for larger-scale chromatography
The Hard series can tolerate pressures up to 0.5 MPa / 5 bar.
Weak Anion Exchange Chromatography
HardIEX DEAE uses DEAE (diethylaminoethyl) as the ion exchange ligand.
DEAE is classified as a weak anion exchange ligand.
Under appropriate pH and buffer conditions, negatively charged biomolecules interact with the positively charged DEAE groups on the chromatography media.
After sample loading and washing, bound molecules can be eluted by changing ionic strength, pH or other chromatographic conditions.
High Dynamic Binding Capacity
HardIEX DEAE provides a specified dynamic binding capacity of:
≥90 mg/mL for ovalbumin
This provides substantial protein loading capability while maintaining the fast-flow characteristics of the high-rigidity agarose matrix.
High Flow Rate and Low Back Pressure
The recommended linear flow rate is:
90–500 cm/h
The mechanically reinforced agarose matrix helps support high-flow operation with relatively low back pressure, making HardIEX DEAE suitable for efficient downstream purification workflows.
Pressure Resistance up to 5 Bar
HardIEX DEAE provides pressure resistance of:
0.5 MPa / 5 bar
The improved mechanical strength is one of the main advantages of the HardIEX platform compared with conventional agarose-based ion exchange media.
pH Stability
HardIEX DEAE provides:
This broad stability range supports process development and cleaning-in-place procedures.
Applications
SHBC HardIEX DEAE can be used for:
Protein purification
Ovalbumin purification
Recombinant protein purification
Biomolecule separation
Weak anion exchange chromatography
Intermediate purification
Downstream bioprocessing
Process-scale purification
Chromatography method development
Purification process scale-up
Product Advantages
HardIEX DEAE combines:
High-rigidity agarose matrix
DEAE weak anion exchange functionality
≥90 mg/mL ovalbumin binding capacity
Recommended flow rates up to 500 cm/h
Low back pressure
Pressure resistance up to 5 bar
Broad pH stability
Good mechanical performance
Suitable for scalable purification
DEAE and Q media provide different ion-exchange characteristics.
Feature | HardIEX DEAE | HardIEX Q |
Exchange Type | Weak Anion Exchange | Strong Anion Exchange |
Ligand | DEAE | Q |
Matrix | High-Rigidity Agarose | High-Rigidity Agarose |
Recommended Flow Rate | 90–500 cm/h | 90–500 cm/h |
Pressure Resistance | 0.5 MPa | 0.5 MPa |
Reference Binding Capacity | ≥90 mg/mL Ovalbumin | ≥100 mg/mL BSA |
Main Difference | pH-dependent weak anion exchange | Strong anion exchange functionality |
Selection should be based on the charge properties of the target molecule and the required purification conditions.
Storage and Handling
Keep the chromatography media in its original container and protect it from contamination during storage and handling.
Before use, equilibrate the packed bed with an appropriate buffer according to the purification process.
Sample conditions, buffer composition, loading amount, flow rate, elution conditions and CIP procedures should be optimized according to the target biomolecule and purification requirements.
Frequently Asked Questions
What is HardIEX DEAE?
HardIEX DEAE is a high-rigidity weak anion exchange chromatography media for protein and biomolecule purification.
What matrix is used?
The media uses a high-rigidity agarose matrix.
What ligand is used?
The ligand is DEAE (diethylaminoethyl).
Is DEAE a strong or weak anion exchanger?
DEAE is a weak anion exchange ligand.
What is the average particle size?
The HardIEX platform has an average particle size of approximately 90 μm.
What is the dynamic binding capacity?
The specified dynamic binding capacity is ≥90 mg/mL for ovalbumin.
What is the recommended flow rate?
The recommended flow rate is 90–500 cm/h.
What is the pressure resistance?
The specified pressure resistance is 0.5 MPa / 5 bar.
What is the working pH range?
The working pH range is 2–9.
What is the long-term pH stability range?
The specified long-term pH stability range is 2–12.
What is the CIP pH range?
The CIP pH range is 2–14.
What package sizes are available?
SHBC offers 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L packages.
What are the SHBC Art.No. numbers?
The corresponding SHBC Art.No. are A2103, A2104, A2105, A2106, A2107, A2108 and A2109.
What brand is HardIEX DEAE?
HardIEX DEAE is supplied under the SHBC brand.
