Product Overview
SHBC His TopAC FF is a fast-flow metal chelation affinity chromatography resin designed for efficient separation and purification of His-tagged proteins.
According to the supplied product information, the TopAC-Ni FF (Fast Flow) metal chelation chromatography medium is prepared by pre-chelating Ni2+ metal ions onto an agarose gel containing chelating ligands such as NTA, IDA and TED.
The resin provides ≈40 mg/mL dynamic binding capacity for His-tagged proteins and is designed to provide high adsorption capacity, good selectivity, easy regeneration and economical operation.
Key Features
Fast Flow affinity chromatography resin
Designed for His-tagged protein purification
Ni2+ metal chelation affinity chromatography
Agarose-based chromatography medium
≈40 mg/mL dynamic binding capacity
High adsorption capacity
Good selectivity
Easy regeneration
Economical purification
Suitable for proteins and peptides
Suitable for biopharmaceutical downstream processing
Suitable for bioengineering applications
Technical Specifications
Parameter | Specification |
|---|
Product Name | His TopAC FF |
Brand | SHBC |
English Name | His-tag Affinity Resin FF |
Series | FF / Fast Flow |
Matrix | Agarose Gel |
Chelating Ligands | NTA / IDA / TED |
Metal Ion | Ni2+ |
Chromatography Mode | Metal Chelation Affinity Chromatography |
Dynamic Binding Load | ≈40 mg/mL (His-tagged protein) |
Main Application | His-tagged Protein Purification |
Package | 25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L |
SHBC Art.No. | Packaging Specification |
|---|
A5015 | 25 mL |
A5016 | 100 mL |
A5017 | 300 mL |
A5018 | 1 L |
A5019 | 5 L |
A5020 | 10 L |
A5021 | 20 L |
His TopAC FF uses Ni2+ metal ions for metal chelation affinity chromatography.
According to the supplied product description, Ni2+ ions are pre-chelated onto agarose-based media containing chelating ligands.
This affinity chromatography approach is designed particularly for the efficient purification of histidine-tagged proteins.
NTA, IDA and TED Chelating Ligands
The supplier information identifies NTA, IDA and TED as chelating ligands used with the agarose gel platform.
These ligands coordinate Ni2+ ions to create the metal chelation affinity environment used for His-tagged protein purification.
This provides selective affinity interaction for downstream protein separation.
Dynamic Binding Capacity
The specified dynamic binding load for His TopAC FF is:
≈40 mg/mL (His-tagged protein)
This value is specifically referenced to His-tagged proteins in the supplied product table.
Actual chromatographic performance may depend on the target protein and purification conditions.
Fast Flow Chromatography Resin
His TopAC FF belongs to the FF (Fast Flow) series.
The resin is designed for affinity purification applications requiring efficient processing of His-tagged proteins and other suitable biomolecules.
Its fast-flow positioning makes it suitable for downstream protein purification workflows and process chromatography applications.
High Adsorption Capacity and Selectivity
According to the supplied product information, TopAC-Ni FF offers:
These characteristics make the media suitable for routine His-tagged protein purification as well as downstream purification processes.
His-Tagged Protein Purification
His TopAC FF is particularly designed for efficient purification of histidine-tagged proteins.
Metal chelation affinity chromatography enables selective capture of His-tagged target proteins, making the resin suitable for recombinant protein purification workflows.
Protein and Peptide Purification
In addition to His-tagged proteins, the supplied series description states that the media is widely used for the separation and purification of proteins and peptides in downstream biopharmaceutical and bioengineering processes.
Its principal highlighted application is the efficient purification of His-tagged proteins.
Applications
SHBC His TopAC FF can be used for:
His-tagged protein purification
Recombinant protein purification
Metal chelation affinity chromatography
Protein separation and purification
Peptide separation and purification
Protein downstream processing
Biopharmaceutical downstream purification
Bioengineering applications
Affinity chromatography
Process purification
Product Advantages
His TopAC FF provides:
≈40 mg/mL His-tagged protein binding capacity
Ni2+ metal chelation
NTA / IDA / TED chelating ligand platform
Agarose-based chromatography medium
Fast Flow series
High adsorption capacity
Good selectivity
Easy regeneration
Economical operation
Multiple package sizes up to 20 L
His TopAC FF for Recombinant Protein Purification
His-tagging is widely used in recombinant protein workflows because it enables affinity-based separation of target proteins.
His TopAC FF provides a Ni2+-based metal chelation affinity platform designed specifically for efficient purification of His-tagged proteins.
The resin can therefore be incorporated into downstream protein purification workflows where selective affinity capture of histidine-tagged targets is required.
Available Package Sizes
His TopAC FF is available in:
25 mL
100 mL
300 mL
1 L
5 L
10 L
20 L
The available package range supports laboratory purification, process development and larger-volume downstream purification.
Frequently Asked Questions
What is His TopAC FF?
His TopAC FF is a Fast Flow metal chelation affinity chromatography resin designed for His-tagged protein purification.
What is the English product name?
The supplied English name is His-tag Affinity Resin FF.
What does FF mean?
FF refers to the supplier's Fast Flow series designation.
The supplied series information specifies Ni2+ metal ions.
What chelating ligands are specified?
The supplied product description identifies NTA, IDA and TED as chelating ligands.
What is the dynamic binding capacity?
The specified dynamic binding load is approximately 40 mg/mL for His-tagged proteins.
What is His TopAC FF mainly used for?
Its principal application is the efficient purification of His-tagged proteins.
Can His TopAC FF be used for recombinant proteins?
Yes. It is suitable for affinity purification workflows involving His-tagged recombinant proteins.
Can it be used for peptide purification?
The supplied series information states that the media can be used for separation and purification of proteins and peptides.
What are the main advantages?
The supplier highlights large adsorption capacity, good selectivity, easy regeneration and low cost.
Is the particle size specified?
No. The supplied table does not specify the particle size for His TopAC FF, so no particle-size value is listed on this page.
What package sizes are available?
Available packages are 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L.
What are the SHBC Art.No. numbers?
The corresponding SHBC Art.No. are A5015, A5016, A5017, A5018, A5019, A5020 and A5021.
What brand is His TopAC FF?
His TopAC FF is supplied under the SHBC brand.
