Products
SHBC provides colored microspheres, fluorescent microspheres, magnetic beads, silica microspheres, chromatography packing microspheres and biological reagents for diagnostic assay development, nucleic acid extraction, protein purification and separation applications.
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RH1489
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5 g
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BioChemika, >99% (TLC)
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IPTG
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367-93-1
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Isopropyl β-D-1-thiogalactopyranoside
Product Overview
RH1489 IPTG (Isopropyl β-D-1-thiogalactopyranoside), CAS 367-93-1, is a high-purity molecular biology reagent supplied as BioChemika grade with purity >99% (TLC).
IPTG is a non-metabolizable analog of allolactose widely used to induce gene expression controlled by the lac operon. It is commonly used in recombinant protein expression systems involving lac-derived promoters.
Key Features
BioChemika grade
Purity >99% (TLC)
CAS 367-93-1
Commonly known as IPTG
Non-metabolizable inducer of the lac operon
Suitable for recombinant protein expression
Suitable for gene expression studies
Suitable for molecular biology research
Suitable for microbiology research
5 g standard packaging
Technical Specifications
Parameter | Specification |
|---|---|
Product Code | RH1489 |
Product Name | IPTG |
Chemical Name | Isopropyl β-D-1-thiogalactopyranoside |
CAS No. | 367-93-1 |
Grade | BioChemika, >99% (TLC) |
Package | 5 g |
Applications
Recombinant Protein Expression
Lac Operon Induction
Gene Expression Research
Molecular Biology
Microbiology Research
Protein Research
Biotechnology Research
Bacterial Expression Systems
Life Science Research
Laboratory Research
IPTG for Recombinant Protein Expression
IPTG is widely used as an inducer in recombinant protein expression systems based on lac-derived regulatory elements.
When added to suitable bacterial expression systems, IPTG interacts with the lac repressor and promotes transcription from lac-controlled promoters.
This makes IPTG an important reagent for recombinant protein production and gene expression research.
IPTG for Lac Operon Induction
IPTG is structurally related to lactose-derived inducers but is resistant to metabolic degradation by β-galactosidase.
Because IPTG is not readily metabolized by the cells, its concentration can remain relatively stable during an induction experiment.
This characteristic makes it useful for controlled induction of lac operon-based expression systems.
IPTG in Molecular Biology Research
IPTG is commonly used in molecular biology laboratories for controlled gene expression.
Typical research applications include bacterial expression of recombinant proteins, promoter studies, gene regulation research and other experiments involving lac or lac-derived expression systems.
The optimal IPTG concentration and induction conditions depend on the expression vector, host strain, target protein and experimental protocol.
IPTG in Bacterial Expression Systems
IPTG is frequently used with E. coli expression systems containing lac-controlled or lac-derived promoters.
Researchers can optimize parameters such as IPTG concentration, induction temperature and induction time according to the characteristics of the target recombinant protein.
Different experimental conditions may significantly affect protein expression level and solubility.
IPTG vs Lactose for Protein Expression
Both IPTG and lactose can be associated with lac-controlled expression systems, but their behavior differs.
IPTG is a synthetic, non-metabolizable inducer, allowing relatively controlled induction without being consumed as a carbon source.
Lactose, in contrast, can be metabolized by suitable cells and may therefore produce different induction characteristics.
The appropriate inducer should be selected according to the expression system and experimental objectives.
Product Advantages
RH1489 IPTG is supplied as BioChemika grade with purity >99% (TLC), making it suitable for molecular biology, recombinant protein expression and biotechnology research.
The standard package is 5 g.
Its high purity makes RH1489 suitable for laboratories requiring IPTG for controlled lac operon induction and related experimental applications.
Storage and Handling
Keep the container tightly sealed.
Protect from moisture and contamination.
Store according to the recommended product conditions.
Prepare solutions using appropriate laboratory procedures.
Refer to the applicable SDS and product documentation for detailed storage and handling information.
Packaging
Standard Packaging: 5 g
Other quantities can be discussed upon request.
Frequently Asked Questions (FAQ)
What is RH1489 IPTG?
RH1489 IPTG, CAS 367-93-1, is Isopropyl β-D-1-thiogalactopyranoside supplied as BioChemika grade with purity >99% (TLC).
What is IPTG used for?
IPTG is primarily used for inducing gene expression in lac operon-based systems, including recombinant protein expression in suitable bacterial hosts.
What does IPTG stand for?
IPTG commonly refers to Isopropyl β-D-1-thiogalactopyranoside.
Is IPTG metabolized by E. coli?
IPTG is commonly described as a non-metabolizable inducer and is resistant to cleavage by β-galactosidase, which helps maintain induction during experiments.
Can IPTG be used for recombinant protein expression?
Yes. IPTG is widely used to induce recombinant protein expression from lac-controlled and lac-derived expression systems.
What is the CAS number of IPTG?
The CAS number of RH1489 IPTG is 367-93-1.
What is the purity of RH1489 IPTG?
RH1489 is supplied as BioChemika grade with purity >99% (TLC).
What packaging is available?
The standard package for RH1489 IPTG is 5 g.