Fluorescent microspheres are obtained by post dyeing latex microspheres, with fluorescent dyes embedded inside the microspheres. The fluorescence signal is stable and will not leak. Therefore, there is no need to worry about changes in fluorescence intensity caused by dye leakage, nor about the impact of dyes on the crosslinking of microspheres and proteins. SHBC has the characteristics of large Stokes shift, no internal quenching effect during aggregation, strong detection signal, less susceptibility to external environmental influences, and stable fluorescence. It is an ideal marker for immunofluorescence quantitative chromatography and is very suitable for the development and application of quantitative chromatography products.