产品分类
量子点聚苯乙烯微球
Quantum dot polystyrene microspheres (HQD) are prepared by microemulsion polymerization of high-brightness CdSe/ZnS quantum dots and polystyrene. The surface of the microspheres is modified with carboxyl functional groups to facilitate antibody coupling. Amplifying the fluorescence signal of quantum dots through nanosphere coating can significantly improve the sensitivity of immune detection.
The fluorescent probe has the characteristics of stable fluorescence signal, excellent colloidal stability, and high biocompatibility, and is especially suitable for the development of fluorescent in vitro diagnostic reagents. . This product can also be used for blood flow measurement, biomarkers, in vivo imaging and calibration of flow cytometers.
I. Description of Quantum Dot Polystyrene Microspheres
Quantum dot polystyrene microspheres (HQD) are prepared by microemulsion polymerization of high-brightness CdSe/ZnS quantum dots and polystyrene. The surface of the microspheres is modified with carboxyl functional groups to facilitate antibody coupling. Amplifying the fluorescence signal of quantum dots through nanosphere coating can significantly improve the sensitivity of immune detection.
-300x237.png)
The fluorescent probe has the characteristics of stable fluorescence signal, excellent colloidal stability, and high biocompatibility, and is especially suitable for the development of fluorescent in vitro diagnostic reagents. . This product can also be used for blood flow measurement, biomarkers, in vivo imaging and calibration of flow cytometers.
Carboxylation-modified microbeads have a high density of free carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules via water-soluble imine-based reagents such as carbodiimide (EDAC).
二、物理数据
1. 材料:聚苯乙烯微球包覆量子点
2.折射率:1.59@589nm,25℃
3、密度:1.05g/cm3
4. 特点: 荧光色
三、分析说明
1.固含量:1%(10mg/ml)
2. 粒径:100nm
3.均匀度:CV<5%
4.发射波长:610nm+10nm
5. 激发波长:<580nm,建议365nm-450nm
6.分散介质:去离子水及微量表面活性剂
7. 稳定性:出厂后2年
IV. Use of Quantum Dot Polystyrene Microspheres
我公司量子点纳米球(HQD610-10)表面富含羧基功能团,可以与含氨基的生物分子,如抗体、多肽、核酸等进行共价偶联,微球表面的羧基在活化剂1-(3-二甲氨基丙基)-3-乙基碳二酰亚胺盐酸盐(EDC)作用下,与氨基发生反应,形成稳定的酰胺键,从而将生物分子共价固定在纳米球表面。
使用注意事项:
○1 使用前请将QDNBs振荡混匀,长期放置产生少量沉淀属正常现象,不影响产品使用。
○2 为了达到更好的偶联效果,蛋白浓度应为0.5-4mg/mL,核酸浓度应为20-100μM,缓冲液中不能有游离氨基,尤其不能使用Tris、甘氨酸、乙酸、柠檬酸缓冲液。
所需材料:
○1 量子点纳米球(10mg/mL)。
○2 反应缓冲液(活化用):pH 4-6
○3活化剂:EDC·HCl。
○4 封闭液:1-2% BSA或casein。
○5保存液(pH7.0-7.5):20-100 mM 硼酸或 Tris,0.9% NaCl,0.5-1% BSA,0.09% NaN3。
参考耦合方案
○1 取约50 μl QDNBs悬浮液,分散于450 μl反应缓冲液中,12000-15000 rpm 离心10-30分钟,弃上清,将QDNBs分散于500 μl反应缓冲液中。
○2 加入偶联剂EDC(10mM)100-50μl,室温(25-37度)混匀,孵育0.5-1h。
○3 Centrifuge at 2000-15000rpm for 10-30min to remove excess coupling agent, etc., then disperse in 500μl reaction buffer, and disperse with ultrasound;
○4 然后加入约100μg抗体(抗体与微球的比例需优化),室温(25-37度)混匀,孵育0.5-1h。
○51 2000-15000 rpm离心10-30分钟,去除游离抗体等;
○6 加入500μl封闭液,用超声波(功率10-20%)分散约10秒,室温(25-37度)下混匀,孵育0.5-1小时。
○7 离心,除去封闭液,分散于200 μl保存液中备用。
本实验方案仅供研究前期参考,具体实验条件请研究者根据自身项目情况进行优化。
产品编号 | 产品名称 | 粒度 |
HQD610-150 | 量子点纳米珠 | 150纳米 |
HQD610-10 | 量子点纳米珠 | 100纳米 |