{"id":1309,"date":"2024-04-25T08:42:28","date_gmt":"2024-04-25T08:42:28","guid":{"rendered":"https:\/\/nanomicronspheres.com\/?post_type=product&#038;p=1309"},"modified":"2025-03-19T09:41:36","modified_gmt":"2025-03-19T09:41:36","slug":"nickel-magnetic-beads","status":"publish","type":"product","link":"https:\/\/nanomicronspheres.com\/zh\/products\/nickel-magnetic-beads\/","title":{"rendered":"\u954d\u78c1\u73e0"},"content":{"rendered":"<h2>1.\u954d\u78c1\u73e0\u6982\u8ff0<\/h2>\n<p>SHBC\u954d\u78c1\u73e0\u5c06\u4f20\u7edf\u78c1\u6027\u5fae\u7403\u4e0e\u954d\u5c42\u6790\u4ecb\u8d28\u76f8\u7ed3\u5408\uff0c\u954d\u78c1\u73e0\u662f\u9488\u5bf9\u5e26\u7ec4\u6c28\u9178\u6807\u7b7e\u7684\u86cb\u767d\u8d28\u5feb\u901f\u5206\u79bb\u800c\u8bbe\u8ba1\u5f00\u53d1\u7684\u65b0\u4e00\u4ee3\u529f\u80fd\u6027\u78c1\u73e0\uff0c\u78c1\u73e0\u4ee5\u743c\u8102\u7cd6\u4e3a\u57fa\u8d28\uff0c\u743c\u8102\u7cd6\u7535\u4e2d\u6027\u3001\u4eb2\u6c34\u6027\u597d\uff0c\u751f\u7269\u76f8\u5bb9\u6027\u597d\uff0c\u907f\u514d\u56e0\u7535\u8377\u6216\u758f\u6c34\u6027\u800c\u5bf9\u86cb\u767d\u8d28\u7684\u975e\u7279\u5f02\u6027\u5438\u9644\u3002<\/p>\n<p>\u4e0e\u4f20\u7edf\u954d\u5c42\u6790\u4ecb\u8d28\u76f8\u6bd4\uff0c\u4f7f\u7528\u954d\u78c1\u73e0\u5206\u79bb\u7eaf\u5316\u5e26\u7ec4\u6c28\u9178\u6807\u7b7e\u7684\u86cb\u767d\uff0c\u65e0\u9700\u5bf9\u7c97\u86cb\u767d\u8fdb\u884c\u4efb\u4f55\u64cd\u4f5c\uff08\u5305\u6db5\u4f53\u9700\u53d8\u6027\uff09\uff0c\u4e0d\u9700\u8981\u67f1\u5c42\u6790\u8bbe\u5907\uff0c\u64cd\u4f5c\u7b80\u4fbf\uff0c\u6027\u4ef7\u6bd4\u66f4\u4f73\u3002<\/p>\n<h2>2.\u4ea7\u54c1\u7279\u70b9<\/h2>\n<p>\uf06c\u4f4e\u975e\u7279\u5f02\u6027\u5438\u9644<br 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width=\"180\">\u914d\u4f53<\/td>\n<td width=\"697\">\u5f02\u620a\u4e8c\u70ef<\/td>\n<\/tr>\n<tr>\n<td width=\"180\">\u88c5\u8f7d\u80fd\u529b<\/td>\n<td width=\"697\">\u226540 mg His\u6807\u7b7e\u86cb\u767d\/mL\uff08\u78c1\u73e0\u6c89\u964d\u4f53\u79ef\uff09<\/td>\n<\/tr>\n<tr>\n<td width=\"180\">\u4f7f\u7528\u6e29\u5ea6<\/td>\n<td width=\"697\">\u4f7f\u7528\u6e29\u5ea6 \u5ba4\u6e29<\/td>\n<\/tr>\n<tr>\n<td width=\"180\">\u50a8\u5b58\u6761\u4ef6<\/td>\n<td width=\"697\">2-8\u2103\uff0c\u4fdd\u5b58\u4e8e20%\u4e59\u9187\u4e2d<\/td>\n<\/tr>\n<tr>\n<td width=\"180\">\u4fdd\u8d28\u671f<\/td>\n<td width=\"697\">5\u5e74<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2>5.\u4f7f\u7528\u65b9\u6cd5\uff08\u53ef\u6839\u636e\u5b9e\u9645\u60c5\u51b5\u8c03\u6574\uff09<\/h2>\n<p>\uf06c\u78c1\u73e0\u4e0e\u86cb\u767d\u8d28\u7ed3\u5408<\/p>\n<p>\u5c06\u9002\u91cf\u78c1\u73e0\u653e\u5165\u79bb\u5fc3\u7ba1\u4e2d\uff0c\u7528Binding Buffer\u6e05\u6d173\u6b21\uff0c\u518d\u7528Binding Buffer\u91cd\u65b0\u5206\u6563\uff0c\u5c06\u7834\u788e\u88c2\u89e3\u540e\u7684\u83cc\u6db2\u4e0e\u78c1\u73e0\u6df7\u5408\uff0c\u5728\u6405\u62cc\u5668\u4e0a\u6df7\u540830\u5206\u949f\u3002<\/p>\n<p>\uf06c\u6e05\u6d17\u78c1\u73e0<br \/>\n30\u5206\u949f\u540e\u5c06\u79bb\u5fc3\u7ba1\u653e\u5728\u78c1\u529b\u5206\u79bb\u5668\u4e0a\uff0c\u5206\u79bb\u78c1\u73e0\uff0c\u53d6\u4e0a\u6e05\u6db2\u8fdb\u884c\u68c0\u6d4b\u3002\u5728\u78c1\u73e0\u4e0a\u52a0\u5165Washing Buffer\uff0c\u4e0a\u4e0b\u7ffb\u8f6c\u6570\u6b21\u4f7f\u78c1\u73e0\u91cd\u65b0\u60ac\u6d6e\uff0c\u78c1\u6027\u5206\u79bb\uff0c\u53d6Washing Buffer\u8fdb\u884c\u68c0\u6d4b\u3002<br \/>\n\u52a0\u5165Washing Buffer\u91cd\u60ac\u78c1\u73e0\uff0c\u5c06\u78c1\u73e0\u8f6c\u79fb\u81f3\u65b0\u7684\u79bb\u5fc3\u7ba1\u4e2d\uff08\u907f\u514d\u86cb\u767d\u6c61\u67d3\uff09\u3002\u78c1\u5206\u79bb\uff0c\u53d6\u51fa\u5e76\u5408\u5e76Washing Buffer\u3002<\/p>\n<p>\uf06c\u76ee\u6807\u86cb\u767d\u6d17\u8131<\/p>\n<p>\u7528\u6237\u53ef\u6839\u636e\u9700\u8981\u6539\u53d8\u6d17\u8131\u4f53\u79ef\u6765\u8c03\u8282\u76ee\u7684\u86cb\u767d\u7684\u6d53\u5ea6\u3002\u52a0\u5165\u6d17\u8131\u7f13\u51b2\u6db2\uff0c\u8f7b\u8f7b\u7ffb\u8f6c\u79bb\u5fc3\u7ba1\u4f7f\u78c1\u73e0\u60ac\u6d6e\uff0c\u5c06\u6d17\u8131\u6db2\u6536\u96c6\u5230\u65b0\u7684\u79bb\u5fc3\u7ba1\u4e2d\uff0c\u5373\u4e3a\u7eaf\u5316\u7684\u76ee\u7684\u86cb\u767d\u3002\u91cd\u590d\u591a\u6b21\uff0c\u786e\u4fdd\u76ee\u7684\u86cb\u767d\u5b8c\u5168\u6d17\u8131\u3002<\/p>\n<p>\uf06c\u78c1\u73e0\u518d\u751f<\/p>\n<p>\u78c1\u73e0\u4f7f\u7528\u4e09\u6b21\u540e\u5bb9\u91cf\u53ef\u80fd\u4f1a\u660e\u663e\u964d\u4f4e\uff0c\u5efa\u8bae\u8fdb\u884c\u518d\u751f\u5904\u7406\u3002\u5982\u9700\u518d\u751f\u78c1\u73e0\uff0c\u53ef\u6309\u5982\u4e0b\u65b9\u6cd5\u64cd\u4f5c\uff1a<\/p>\n<p>a) \u5c06\u78c1\u73e0\u5206\u6563\u4e8e\u542b\u67090.1 M EDTA\u7684\u78f7\u9178\u76d0\u7f13\u51b2\u6db2\uff0820 mM\uff0cpH 7.4\uff09\u4e2d\uff0c\u5ba4\u6e29\u632f\u644710\u5206\u949f\uff0c\u78c1\u529b\u5438\u5f03\u4e0a\u6e05\uff0c\u91cd\u590d1\u6b21\u3002<\/p>\n<p>b) \u7528\u9ad8\u7eaf\u6c34\u6e05\u6d17\u78c1\u73e0\u6570\u6b21\uff0c\u786e\u4fdd\u6eb6\u6db2\u4e2d\u4e0d\u542bEDTA\u3002<\/p>\n<p>c) \u75280.5 M NaOH\u548c2 M NaCl\u6eb6\u6db2\u6d17\u6da4\u78c1\u73e0\uff0c\u5ba4\u6e29\u632f\u64475\u5206\u949f\uff0c\u78c1\u5438\u9644\uff0c\u5f03\u4e0a\u6e05\uff0c\u7528\u9ad8\u7eaf\u6c34\u6d17\u6da4\u78c1\u73e0\uff0c\u76f4\u81f3\u6d17\u6db2\u5448\u4e2d\u6027\u3002<\/p>\n<p>d) \u75280.1 M NiSO4\u6eb6\u6db2\u5206\u6563\u78c1\u73e0\uff0c\u5ba4\u6e29\u632f\u644710\u5206\u949f\uff0c\u78c1\u529b\u5438\u53bb\u4e0a\u6e05\uff0c\u7528\u9ad8\u7eaf\u6c34\u591a\u6b21\u6e05\u6d17\u78c1\u73e0\uff0c\u76f4\u81f3\u6eb6\u6db2\u4e2d\u65e0Ni\u79bb\u5b50\uff0c\u5c06\u78c1\u73e0\u4fdd\u5b58\u4e8e20%\u4e59\u9187\u4e2d\u3002<\/p>\n<h2>6.\u6ce8\u610f\u4e8b\u9879<\/h2>\n<p>\uf06c\u78c1\u73e0\u5bc6\u5ea6\u8f83\u5927\uff0c\u957f\u65f6\u95f4\u653e\u7f6e\u5bb9\u6613\u4e0b\u6c89\uff0c\u56e0\u6b64\u4f7f\u7528\u524d\u8bf7\u5145\u5206\u6447\u5300\uff0c\u4ee5\u83b7\u5f97\u5747\u5300\u7684\u78c1\u73e0\u60ac\u6d6e\u6db2\u3002<br \/>\n\u9644\u5f55\uff08\u6eb6\u5242\u8010\u53d7\u6027\u8868\uff09<\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"241\">\u6eb6\u5242\u540d\u79f0<\/td>\n<td width=\"171\">\u8010\u53d7\u6d53\u5ea6<\/td>\n<td width=\"206\">\u00a0\u6eb6\u5242\u540d\u79f0<\/td>\n<td width=\"206\">\u00a0\u8010\u53d7\u6d53\u5ea6<\/td>\n<td width=\"206\">\u6eb6\u5242\u540d\u79f0<\/td>\n<td width=\"206\">\u8010\u53d7\u6d53\u5ea6<\/td>\n<\/tr>\n<tr>\n<td width=\"241\">\u6570\u636e\u7ec8\u7aef<\/td>\n<td width=\"171\">5 \u6beb\u7c73<\/td>\n<td width=\"206\">\u6d77\u536b\u5341\u4e16<\/td>\n<td width=\"206\">2%<\/td>\n<td width=\"206\">Tris-HCl pH7.4<\/td>\n<td width=\"206\">100 \u6beb\u7c73<\/td>\n<\/tr>\n<tr>\n<td width=\"241\">\u6570\u7801\u5730\u9762\u7535\u89c6<\/td>\n<td width=\"171\">5 \u6beb\u7c73<\/td>\n<td width=\"206\">\u5410\u6e29 20<\/td>\n<td width=\"206\">2 %<\/td>\n<td width=\"206\">\u54aa\u5511<\/td>\n<td width=\"206\">1 \u7c73<\/td>\n<\/tr>\n<tr>\n<td width=\"241\">\u6c2f\u4e59\u70ef<\/td>\n<td width=\"171\">5 \u6beb\u7c73<\/td>\n<td width=\"206\">NP-40<\/td>\n<td width=\"206\">2 %<\/td>\n<td width=\"206\">\u4e59\u9187<\/td>\n<td width=\"206\">20 %<\/td>\n<\/tr>\n<tr>\n<td width=\"241\">\u5c3f\u7d20<\/td>\n<td width=\"171\">8 \u7c73<\/td>\n<td width=\"206\">\u4e9a\u592a\u7ecf\u793e\u4f1a<\/td>\n<td width=\"206\">1 %<\/td>\n<td width=\"206\">\u4e59\u4e8c\u80fa\u56db\u4e59\u9178<\/td>\n<td width=\"206\">1 \u6beb\u7c73<\/td>\n<\/tr>\n<tr>\n<td width=\"241\">\u76d0\u9178\u80cd<\/td>\n<td width=\"171\">6 \u7c73<\/td>\n<td width=\"206\">PB pH \u503c 7.4<\/td>\n<td width=\"206\">50 \u6beb\u7c73<\/td>\n<td width=\"206\">\u67e0\u6aac\u9178\u76d0<\/td>\n<td width=\"206\">60 \u6beb\u7c73<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<table style=\"border-collapse: collapse; width: 535.52pt;\" border=\"0\" width=\"1428\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr style=\"height: 15.75pt;\">\n<td class=\"et2\" style=\"height: 15.75pt; width: 181.80pt;\" width=\"484\" height=\"42\">\u4ea7\u54c1\u7f16\u53f7<\/td>\n<td class=\"et4\" style=\"width: 230.30pt;\" width=\"614\">\u4ea7\u54c1\u540d\u79f0<\/td>\n<td class=\"et4\" style=\"width: 123.55pt;\" width=\"329\">\u7c92\u5ea6<\/td>\n<\/tr>\n<tr style=\"height: 13.50pt;\">\n<td class=\"et3\" style=\"height: 13.50pt;\" height=\"36\">MagNi1UM-10<\/td>\n<td class=\"et3\">\u954d\u78c1\u73e0<\/td>\n<td class=\"et3\">1\u5fae\u7c73<\/td>\n<\/tr>\n<tr style=\"height: 13.50pt;\">\n<td class=\"et3\" style=\"height: 13.50pt;\" height=\"36\">MagNi3UM-10<\/td>\n<td class=\"et3\">\u954d\u78c1\u73e0<\/td>\n<td class=\"et3\">3\u5fae\u7c73<\/td>\n<\/tr>\n<tr style=\"height: 13.50pt;\">\n<td class=\"et3\" style=\"height: 13.50pt;\" height=\"36\">MagNi5UM-10<\/td>\n<td class=\"et3\">\u954d\u78c1\u73e0<\/td>\n<td class=\"et3\">5\u5fae\u7c73<\/td>\n<\/tr>\n<tr style=\"height: 13.50pt;\">\n<td class=\"et3\" style=\"height: 13.50pt;\" height=\"36\">MagNi10UM-10<\/td>\n<td class=\"et3\">\u954d\u78c1\u73e0<\/td>\n<td class=\"et3\">10\u5fae\u7c73<\/td>\n<\/tr>\n<tr style=\"height: 13.50pt;\">\n<td class=\"et3\" style=\"height: 13.50pt;\" height=\"36\">MagNi20UM-10<\/td>\n<td class=\"et3\">\u954d\u78c1\u73e0<\/td>\n<td class=\"et3\">20\u5fae\u7c73<\/td>\n<\/tr>\n<tr style=\"height: 13.50pt;\">\n<td class=\"et3\" style=\"height: 13.50pt;\" height=\"36\">MagNi30UM-10<\/td>\n<td class=\"et3\">\u954d\u78c1\u73e0<\/td>\n<td class=\"et3\">30\u5fae\u7c73<\/td>\n<\/tr>\n<tr style=\"height: 13.50pt;\">\n<td class=\"et3\" style=\"height: 13.50pt;\" height=\"36\">MagNi40UM-10<\/td>\n<td class=\"et3\">\u954d\u78c1\u73e0<\/td>\n<td class=\"et3\">40\u5fae\u7c73<\/td>\n<\/tr>\n<tr style=\"height: 13.50pt;\">\n<td class=\"et3\" style=\"height: 13.50pt;\" height=\"36\">MagNi50UM-10<\/td>\n<td class=\"et3\">\u954d\u78c1\u73e0<\/td>\n<td class=\"et3\">50\u03bcm<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>","protected":false},"excerpt":{"rendered":"<p>SHBC\u954d\u78c1\u73e0\u5c06\u4f20\u7edf\u78c1\u6027\u5fae\u7403\u4e0e\u954d\u5c42\u6790\u4ecb\u8d28\u76f8\u7ed3\u5408\uff0c\u954d\u78c1\u73e0\u662f\u9488\u5bf9\u5e26\u7ec4\u6c28\u9178\u6807\u7b7e\u7684\u86cb\u767d\u8d28\u5feb\u901f\u5206\u79bb\u800c\u8bbe\u8ba1\u5f00\u53d1\u7684\u65b0\u4e00\u4ee3\u529f\u80fd\u6027\u78c1\u73e0\uff0c\u78c1\u73e0\u4ee5\u743c\u8102\u7cd6\u4e3a\u57fa\u8d28\uff0c\u743c\u8102\u7cd6\u7535\u4e2d\u6027\u3001\u4eb2\u6c34\u6027\u597d\uff0c\u751f\u7269\u76f8\u5bb9\u6027\u597d\uff0c\u907f\u514d\u56e0\u7535\u8377\u6216\u758f\u6c34\u6027\u800c\u5bf9\u86cb\u767d\u8d28\u7684\u975e\u7279\u5f02\u6027\u5438\u9644\u3002<\/p>","protected":false},"featured_media":1703,"template":"","meta":{"nf_dc_page":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center 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