{"id":6278,"date":"2025-08-04T05:05:42","date_gmt":"2025-08-04T05:05:42","guid":{"rendered":"https:\/\/nanomicronspheres.com\/?post_type=product&p=6278"},"modified":"2025-11-10T06:35:45","modified_gmt":"2025-11-10T06:35:45","slug":"polymer-reversed-phase-chromatography-packing-microspheres","status":"publish","type":"product","link":"https:\/\/nanomicronspheres.com\/zh\/products\/polymer-reversed-phase-chromatography-packing-microspheres\/","title":{"rendered":"Polymer Reversed-phase Chromatography Packing Microspheres"},"content":{"rendered":"

Particle size is highly uniform
\nControlling the particle size and uniformity of the beads is critical for chromatographic applications, as the particle size and particle size distribution of the beads can often determine the efficacy. In chromatographic separation, the longitudinal diffusion of solutes is the main reason for the broadening of chromatographic bands and peaks. For beads of a specific size, columns packed with uniform beads can effectively narrow the bands and peaks, and also obtain the lowest backpressure, resulting in high column efficiency and high resolution.
\nSHBC can provide polymer reversed-phase chromatography packing materials of any size and uniform particle size from 3\u03bcm to 1000\u03bcm, which customers can choose from and Zhiyi can customize according to customer needs. Figure 1 shows a scanning electron microscopy of a series of polymer reversed-phase chromatography chemistry with different particle sizes.
\nThe particle size distribution of SHBC polymer chromatography resins was measured using the Beckman Coulter Counter, as shown in Figure 2, with a CV<3% coefficient of variation. Results Mingzhiyi Technology’s polymer chromatography chemistry chemistry has a high degree of uniformity.<\/p>\n

\"Polymer
Figure 1. Scanning electron microscopy of polymer reversed-phase chromatography packing materials with different particle sizes<\/figcaption><\/figure>\n
\"Figure
Figure 2. Particle size distribution map of polymer reversed-phase chromatography packing<\/figcaption><\/figure>\n

Optimized hole structure
\nThe pore size of the microspheres has a great relationship with the specific surface volume of the chromatographic packing material, and the smaller the pore size, the larger the specific surface area. The target separated material is a small molecule, and a chromatographic packing material with a small pore size is usually selected. If the target separated material is a biological macromolecule, a chromatographic packing material with a larger pore size should be selected. For specific target separations, choosing an optimized pore size structure chromatographic chemistry chemistry chemistry can not only increase sample loading, but also increase the purity of the separation.
\nSHBC can provide chromatographic chemistry chemistry chemistry in different pore sizes, including 100\u00c5, 300\u00c5, 500\u00c5, and 1000\u00c5. Figure 3 shows the scanning electron microscopy of four homogeneous polymer reversed-phase chromatography packing materials with different pore sizes.<\/p>\n

\"Figure
Figure 3. Scanning electron microscopy of four homogeneous polymer reversed-phase chromatography packing materials with different pore sizes<\/figcaption><\/figure>\n

Batch-to-batch stability
\nSHBC uses leading large-scale polymer microsphere production technology to ensure batch stability of chromatographic packing materials. We evaluate the performance of each batch of products, such as particle size and distribution. Figure 4 shows the performance of different batches of filler products, indicating that our large-scale production of fillers has good stability. Figure 5 shows the filling of 18 batches
\nThe material should be used for the separation and purification of proteins, and the chromatogram further illustrates the stability of our chromatographic packing batches.<\/p>\n

\"Figure
Figure 4 The particle size and particle size distribution stability of different batches of filler products<\/figcaption><\/figure>\n

\"\"<\/p>\n

Column: 4.6 mm I.D. x 250 mm, KBsphere\u00ae 10PS-300<\/p>\n

Mobile Phase A: 0.1% trifluoroacetic acid in water
\nMobile phase B: 0.1% acetonitrile solution of trifluoroacetic acid
\nGradient: 20%B to 40%B, 20min; 40% B 10min<\/p>\n

flow rate: 1 ml\/min; 80%B to 95%B, 10min
\nDetection wavelength: UV @ 280nm<\/p>\n

High chemical stability<\/h2>\n

SHBC series polymer reversed-phase chromatography chemistry chemistry is composed of highly Crosslinked polystyrene\/divinylbenzene or highly crosslinked polymethacrylate with good temperature resistance over the full pH range (pH 1-14). The structural stability and performance stability of the microspheres can be maintained in extremely acidic solutions (such as 1M HCl) or extreme basic solutions (such as 1M NaOH) and organic solvents (including ethanol, methanol, acetonitrile, isopropyl alcohol, acetone, DMSO, tetrahydrofuran, etc.).<\/p>\n

Choice of different polar chromatographic packing materials<\/h3>\n

SHBC series polymer reversed-phase chromatography chemistry chemistry is composed of highly translinked polystyrene\/divinylbenzene or highly cross-linked polymethacrylate. By adjusting the ratio of different matrices, we can provide polymer reversed-phase chromatography chemistry chemistry with different polarities, including PS, PSM and PMM
\nPolar filler, the polarity of the packing material is gradually enhanced.<\/p>\n

Low backpressure and high column efficiency<\/h3>\n

SHBC series polymer reversed-phase chromatography packing materials have uniform particle size height and strong rigidity, so the column bed of the packed column is stable, and the number of plates with low SHBC10PS-300 backpressure chemistry can reach 50,000 N\/m.<\/p>\n

Multiple options for chromatographic chemistry<\/h3>\n

The purification<\/span> process<\/span> is<\/span> basically<\/span> divided<\/span> into<\/span> three<\/span> separation<\/span> steps<\/span>:<\/span> coarse<\/span> extraction<\/span>,<\/span> intermediate<\/span> purification<\/span> and<\/span> fine<\/span> purification<\/span>.<\/span> The S<\/span>HBC s<\/span>eries o<\/span>f p<\/span>olymer r<\/span>eversed-p<\/span>hase c<\/span>hromatography c<\/span>hemistry o<\/span>ffers a<\/span> c<\/span>hoice o<\/span>f s<\/span>eparation p<\/span>rocesses f<\/span>rom c<\/span>oarse e<\/span>xtraction, <\/span>i<\/span>ntermediate p<\/span>urification, <\/span>\u4e00\u4e2a<\/span>nd f<\/span>ine p<\/span>urification o<\/span>f samples.
\n<\/span>
\n<\/span>Coarse <\/span>extraction:<\/span> refers <\/span>to <\/span>the <\/span>capture <\/span>of the <\/span>target <\/span>component <\/span>from <\/span>the <\/span>sample <\/span>\u4e00\u4e2and <\/span>the <\/span>preliminary <\/span>separation <\/span>of the <\/span>target <\/span>object,<\/span> which <\/span>is <\/span>a low-<\/span>r<\/span>esolution <\/span>separation <\/span>and purification,<\/span> \u4e00\u4e2and <\/span>the purity <\/span>can <\/span>generally <\/span>reach <\/span>70~90%<\/span>.<\/span> Choose <\/span>from <\/span>100 <\/span>\u03bcm <\/span>SHBC <\/span>Series <\/span>chromatography <\/span>packs.<\/span><\/p>\n

Intermediate purification: refers to removing most of the impurities from the captured target, which is a medium and high resolution separation purification, and the purity can reach 90~98%. Choose from 30 or 40 \u03bcm SHBC Series chromatography chemistry.
\nFine purification: refers to the target object reaching the expected purity, removing the substance similar to the target object structure, which is a high-resolution separation purification, and the purity can reach more than 98~99%. Choose from a 10 \u03bcm series of chromatography chemistry.<\/p>\n

Specification of chromatography packing microspheres<\/h2>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u4ea7\u54c1\u540d\u79f0<\/td>\nParticle size\uff08\u03bcm\uff09<\/td>\naperture\uff08\u00c5\uff09<\/td>\nMedia composition<\/td>\n<\/tr>\n
PS\/DVB3UM<\/td>\n3<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPS\/DVB<\/td>\n<\/tr>\n
PS\/DVB5UM<\/td>\n5<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPS\/DVB<\/td>\n<\/tr>\n
PS\/DVB10UM<\/td>\n10<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPS\/DVB<\/td>\n<\/tr>\n
PS\/DVB15UM<\/td>\n15<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPS\/DVB<\/td>\n<\/tr>\n
PS\/DVB25UM<\/td>\n25<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPS\/DVB<\/td>\n<\/tr>\n
PS\/DVB30UM<\/td>\n30<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPS\/DVB<\/td>\n<\/tr>\n
PS\/DVB40UM<\/td>\n40<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPS\/DVB<\/td>\n<\/tr>\n
PS\/DVB50UM<\/td>\n50<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPS\/DVB<\/td>\n<\/tr>\n
PS\/DVB60UM<\/td>\n60<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPS\/DVB<\/td>\n<\/tr>\n
PS\/DVB100UM<\/td>\n100<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPS\/DVB<\/td>\n<\/tr>\n
PS\/DVB200UM<\/td>\n200<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPS\/DVB<\/td>\n<\/tr>\n
PSM\/DVB3UM<\/td>\n3<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPoly DVB\/acrylate<\/td>\n<\/tr>\n
PSM\/DVB5UM<\/td>\n5<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPoly DVB\/acrylate<\/td>\n<\/tr>\n
PSM\/DVB10UM<\/td>\n10<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPoly DVB\/acrylate<\/td>\n<\/tr>\n
PSM\/DVB15UM<\/td>\n15<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPoly DVB\/acrylate<\/td>\n<\/tr>\n
PSM\/DVB25UM<\/td>\n25<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPoly DVB\/acrylate<\/td>\n<\/tr>\n
PSM\/DVB30UM<\/td>\n30<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPoly DVB\/acrylate<\/td>\n<\/tr>\n
PSM\/DVB40UM<\/td>\n40<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPoly DVB\/acrylate<\/td>\n<\/tr>\n
PSM\/DVB50UM<\/td>\n50<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPoly DVB\/acrylate<\/td>\n<\/tr>\n
PSM\/DVB60UM<\/td>\n60<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPoly DVB\/acrylate<\/td>\n<\/tr>\n
PSM\/DVB100UM<\/td>\n100<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPoly DVB\/acrylate<\/td>\n<\/tr>\n
PSM\/DVB200UM<\/td>\n200<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPoly DVB\/acrylate<\/td>\n<\/tr>\n
PMM3UM<\/td>\n3<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPolyacrylate<\/td>\n<\/tr>\n
PMM5UM<\/td>\n5<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPolyacrylate<\/td>\n<\/tr>\n
PMM10UM<\/td>\n10<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPolyacrylate<\/td>\n<\/tr>\n
PMM15UM<\/td>\n15<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPolyacrylate<\/td>\n<\/tr>\n
PMM25UM<\/td>\n25<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPolyacrylate<\/td>\n<\/tr>\n
PMM30UM<\/td>\n30<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPolyacrylate<\/td>\n<\/tr>\n
PMM40UM<\/td>\n40<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPolyacrylate<\/td>\n<\/tr>\n
PMM50UM<\/td>\n50<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPolyacrylate<\/td>\n<\/tr>\n
PMM60UM<\/td>\n60<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPolyacrylate<\/td>\n<\/tr>\n
PMM100UM<\/td>\n100<\/td>\nnon-porous,100, 300, 500, 1000<\/td>\nPolyacrylate<\/td>\n<\/tr>\n
PMM200UM<\/td>\n200<\/td>\nnon-porous, 100, 300, 500, 1000<\/td>\nPolyacrylate<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>","protected":false},"excerpt":{"rendered":"

SHBC uses the world’s leading microsphere synthesis technology to prepare two complementary high-performance polymer reversed-phase chromatography chemistry chemistries based on polystyrene\/divinylbenzene and polymethacrylate matrix, which have the characteristics of strong rigidity, uniform particle size, low backpressure, and acid and alkali resistance. SHBC polymer reversed-phase chromatography chemistry chemistry chemistry is available in different particle size sizes (3 \u03bcm, 5 \u03bcm, 10 \u03bcm, 15 \u03bcm, 20 \u03bcm, 25 \u03bcm, 30 \u03bcm, 40 \u03bcm and 50 \u03bcm) and optimized pore size structures (100 \u00c5, 300 \u00c5, 500 \u00c5 and 1000 \u00c5 pore sizes) and non-porous structures to meet a wide range of needs from laboratory analysis to large-scale industrial separations such as natural products, antibiotics, organic compounds, peptides, protees and oligonucleotides Efficient separation of sublimina.
\nFeatures of polymer reversed-phase chromatography packing materials:
\n\u2022 1. Spherical shape, uniform particle size and height
\n\u2022 2. High chemical stability and wide pH range (pH=1-14)
\n\u2022 3. Optimized pore size structure
\n\u2022 4. High specific surface volume and high load
\n\u2022 5. High mechanical strength
\n\u2022 6. Low backpressure and high column efficiency
\n\u2022 7. Selection of different polar substrates<\/p>","protected":false},"featured_media":6279,"template":"","meta":{"nf_dc_page":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"product_brand":[],"product_cat":[39],"product_tag":[],"class_list":{"0":"post-6278","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-polymer-reversed-phase-chromatography-packing-microspheres","7":"desktop-align-left","8":"tablet-align-left","9":"mobile-align-left","11":"first","12":"instock","13":"shipping-taxable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/nanomicronspheres.com\/zh\/wp-json\/wp\/v2\/product\/6278","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanomicronspheres.com\/zh\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/nanomicronspheres.com\/zh\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nanomicronspheres.com\/zh\/wp-json\/wp\/v2\/media\/6279"}],"wp:attachment":[{"href":"https:\/\/nanomicronspheres.com\/zh\/wp-json\/wp\/v2\/media?parent=6278"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/nanomicronspheres.com\/zh\/wp-json\/wp\/v2\/product_brand?post=6278"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/nanomicronspheres.com\/zh\/wp-json\/wp\/v2\/product_cat?post=6278"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/nanomicronspheres.com\/zh\/wp-json\/wp\/v2\/product_tag?post=6278"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}