Carboxy Magnetic Beads

1 Product Overview
MagCOOH carboxyl magnetic beads use superparamagnetic Fe3O4 as the core and are modified with carboxyl functional groups on the surface. They have the characteristics of high magnetic content and monodispersity. Under the action of specific reagents, the abundant carboxyl functional groups can covalently bind biological ligands such as proteins to the surface of magnetic beads, thereby achieving rapid separation and enrichment of target substances from samples. This is an important issue in molecular biology, An important carrier for research in medicine and other related fields.
2 product features
Fast magnetic response and highly uniform particle size
Small batch-to-batch variation and high repeatability
Good biocompatibility and low cytotoxicity
Convenient to couple with a variety of biomolecules and easy to operate
3 Scope of application
Targeted drugs
Biologically active substance detection
Sample enrichment and separation
4 Product parameters and specifications
Particle size: 200nm, 1um, 3um, 5um, 10um, 20um, 30um, 50um
Particle size uniformity: CV<5%
Concentration: 50 mg/mL
Solvent: DI Water
Surface functional group: carboxyl group (-COOH)
Carboxyl density: 200 μmol/g
Applicable solutions: PBS, Tris-HCl, H2O
Iron (Fe) content: 30%
5. Coupling method with biomolecules (EDC/NHS method)
Activation of carboxyl groups on the surface of magnetic beads
After mixing the magnetic beads, remove an appropriate amount of magnetic beads into a centrifuge tube, magnetically remove the supernatant, and wash the magnetic beads with the prepared 20 mM MEST buffer (20 mM MES buffer, pH 5.0, 0.05% Tween 20) 3 Second-rate. Take an appropriate amount of newly prepared EDC solution and an equal volume of NHS solution to disperse the magnetic beads, and activate them at 25°C for 3 hours.
Magnetic beads coupled with biomolecules
Magnetically absorb the magnetic beads, discard the supernatant, disperse an appropriate amount of biomolecules in a pH 8.0 buffer, redisperse the magnetic beads in this solution, and couple at 25°C for 3 hours or 4°C overnight. After coupling, magnetically absorb the beads, remove the supernatant, add buffer containing 1% BSA, redisperse the beads, and couple at 25°C for 1 hour to block unreacted activated carboxyl groups.
After blocking, magnetically absorb the beads, remove the supernatant, wash the beads 3 times with 20 mM pH 7.4 PBS buffer, redisperse, and store at 4°C. If necessary, add 0.02% sodium azide to inhibit bacterial growth.
6 things to note
Magnetic beads have a high density and will easily sink if left standing for a long time. Therefore, please shake well before use to obtain a uniform magnetic bead suspension.
7 Storage conditions and expiry date
Storage conditions: Store at 2~8℃, do not freeze or dry.