Tosyl Magnetic Beads

1 Product Overview
SHBC Tosyl p-toluenesulfonyl magnetic beads use superparamagnetic Fe3O4 as the core and are modified with p-toluenesulfonyl functional groups on the surface. They have the characteristics of high magnetic content and monodispersity. Under the action of specific reagents, the abundant p-toluenesulfonyl functional groups can covalently bind biological ligands such as proteins to the surface of magnetic beads, thereby achieving rapid separation and enrichment of target substances from samples. It is a molecule An important carrier for research in biology, medicine and other related fields.
2 product features
Fast magnetic response and good monodispersity
Small batch-to-batch variation and high repeatability
Good biocompatibility and low cytotoxicity
Convenient to couple with a variety of biomolecules and easy to operate
3 Scope of application
Biologically active substance detection
Sample enrichment and separation
4 Product parameters and specifications
Particle size: 1 μm
Concentration: 50 mg/mL
Preservation solution: isopropyl alcohol
Surface ligand: p-toluenesulfonyl group
Group density: ≥250 μmol/g
Applicable solutions: PBS, H2O
Iron (Fe) content: 30%
5. Coupling methods with biomolecules
Take an appropriate amount of magnetic beads, wash them several times with coupling buffer until the isopropyl alcohol is completely removed, and set aside for use. Disperse an appropriate amount of biomolecules in a pH 8.0 carbonate buffer, redisperse the magnetic beads in this solution, and couple at 37°C for 2 hours or 4°C overnight. After coupling, magnetically absorb the beads, remove the supernatant, add buffer containing 1% BSA, redisperse the beads, and couple at 25°C for 1 hour to block unreacted p-toluenesulfonyl groups.
After blocking, magnetically absorb the beads, remove the supernatant, wash the beads 3 times with 20 mM pH 7.4 PBS buffer, redisperse, and store at 4°C. If necessary, add 0.02% sodium azide to inhibit bacterial growth.
6 things to note
Magnetic beads have a high density and will easily sink if left standing for a long time. Therefore, please shake well before use to obtain a uniform magnetic bead suspension.
7 Storage conditions and expiry date
Storage conditions: Store at 2~8℃, do not freeze or dry.