100um Carboxy Silica Microspheres CSi100UM-10 5%

100um Carboxyl Silica Microspheres for Immunoassay R&D

SHBC CSi100UM-10 Carboxyl Silica Microspheres are 100 µm silica particles with surface carboxyl groups and a solid content of 5%.

The functional carboxyl surface supports covalent immobilization of antibodies, antigens, proteins, peptides and amino-modified biomolecules.

The large particle size provides convenient washing, filtration and recovery for solid-phase immunoassay research, affinity capture and bioseparation development.

Product Specifications

Batch-specific dispersion medium, particle size distribution and surface carboxyl content should be confirmed in the applicable product specification.

Key Features and Advantages

Reactive Carboxyl Surface

Surface carboxyl groups can be activated for covalent coupling with primary amino groups on antibodies, proteins and other biomolecules.

Large 100um Particle Size

The large particle size enables convenient observation, filtration, sedimentation, washing and recovery during assay development.

Easy Solid-Phase Separation

The microspheres can be separated using centrifugation, filtration, sedimentation or mesh retention without magnetic equipment.

Stable Silica Matrix

The silica structure provides good mechanical and chemical stability for repeated washing, surface modification and biomolecule immobilization.

Convenient 5% Suspension

The standard 5% solid content supports laboratory evaluation, coupling optimization, pilot formulation and batch production.

Scalable Manufacturing

SHBC supports samples, customized specifications, pilot orders and stable bulk supply.

Carboxyl Surface for Biomolecule Coupling

CSi100UM-10 can be evaluated for immobilizing:

• Antibodies

• Antigens

• Proteins

• Peptides

• Enzymes

• Amino-modified oligonucleotides

• Affinity ligands

• Other amine-containing biomolecules

EDC/NHS or EDC/Sulfo-NHS chemistry can be used to activate the surface carboxyl groups. The activated groups react with primary amines to form stable amide bonds.

Coupling efficiency depends on buffer composition, pH, reagent concentration, biomolecule loading and reaction time.

Immunoassay and Bioseparation Applications

CSi100UM-10 can be evaluated for:

• Solid-phase immunoassays

• Antibody immobilization

• Antigen coating

• Protein capture

• Affinity separation

• Particle-capture assay development

• Filtration-assisted immunoreactions

• Column-based detection

• Flow-through assay development

• Enzyme immobilization

• Biomolecule conjugation

• Biosensor carrier development

• Diagnostic reagent research

• Laboratory purification processes

Application compatibility should be verified according to the instrument, reaction vessel and detection method.

Why Choose 100um Carboxyl Silica Microspheres?

Compared with smaller silica particles, 100 µm microspheres provide easier physical handling and recovery.

Key benefits include:

• Convenient filtration

• Rapid sedimentation

• Easy microscopic observation

• Reduced particle loss during washing

• Simple buffer exchange

• Flexible carboxyl coupling chemistry

• Suitability for fixed solid-phase formats

• Convenient research-to-production scale-up

Because 100 µm particles settle faster than conventional suspension-assay microspheres, sufficient mixing should be maintained during the reaction.

Recommended EDC/NHS Coupling

A typical development process may include:

1. Resuspend and wash the microspheres.

2. Transfer them into an amine-free activation buffer.

3. Add freshly prepared EDC and NHS or Sulfo-NHS.

4. Activate the surface carboxyl groups.

5. Remove excess activation reagents.

6. Add the antibody, antigen or target protein.

7. Incubate under gentle mixing.

8. Block remaining reactive sites.

9. Wash the conjugated microspheres.

10. Resuspend them in a suitable storage buffer.

All reagent ratios and reaction conditions should be optimized for the selected biomolecule.

Handling and Process Optimization

• Mix gently before sampling.

• Resuspend settled particles completely.

• Avoid allowing the microspheres to dry.

• Use transfer tools suitable for 100 µm particles.

• Avoid narrow tubing or dispensing nozzles.

• Use gentle mixing during coupling.

• Select suitable centrifugation or filtration conditions.

• Avoid primary-amine buffers during EDC/NHS activation.

• Optimize blocking and washing conditions.

• Avoid repeated freeze-thaw cycles.

• Follow batch-specific storage instructions.

Before scale-up, confirm particle transfer, sedimentation, recovery rate, coupling efficiency and assay performance.

Customization and Bulk Production

Shanghai SanYu Biotechnology Co., Ltd. provides carboxyl silica microspheres for biotechnology companies, research institutions and diagnostic reagent manufacturers.

Customization options may include:

• Different particle sizes

• Different solid contents

• Carboxyl group density adjustment

• Custom dispersion media

• Fluorescent modification

• Antibody or protein conjugation

• Custom packaging

• Pilot-scale manufacturing

• Bulk production

• OEM and ODM services

Customization feasibility depends on the required specification, application and order volume.

Why Choose SHBC?

• Specialized microsphere manufacturer

• Controlled 100 µm particle size

• Reactive carboxyl surface

• Standard 5% solid content

• Easy filtration and recovery

• Suitable for solid-phase carrier research

• Support for biomolecule coupling

• Laboratory samples available

• Pilot-scale production support

• Stable batch-to-batch supply

• Custom surface chemistry

• OEM and ODM manufacturing

Quality Documentation

Available documentation may include:

• Certificate of Analysis

• Safety Data Sheet

• Product specification

• Particle size information

• Solid-content information

• Batch identification

• Handling guidance

• Storage recommendations

Please confirm the available test items before placing a bulk order.

Frequently Asked Questions

What is CSi100UM-10?

CSi100UM-10 is a 100 µm carboxyl-functionalized silica microsphere suspension with a solid content of 5%, manufactured by SHBC.

What can be coupled to the carboxyl surface?

Antibodies, antigens, proteins, peptides, enzymes and amino-modified oligonucleotides can be evaluated for covalent coupling.

Which coupling method is commonly used?

EDC/NHS or EDC/Sulfo-NHS chemistry can be evaluated to activate the carboxyl surface for reaction with primary amino groups.

What is the advantage of the 100um size?

The large particles are easy to observe, wash, filter and recover during solid-phase assay and separation research.

Are the microspheres suitable for suspension immunoassays?

The particles sediment faster than conventional immunoassay microspheres. They are better suited to mixed batch reactions, filtration-assisted assays and fixed solid-phase formats.

How can the particles be separated?

Centrifugation, filtration, sedimentation or mesh retention can be evaluated according to the assay design.

Is blocking required after coupling?

Blocking should be evaluated to reduce residual reactive groups and nonspecific adsorption.

Can SHBC customize the specification?

Yes. Particle size, concentration, carboxyl density, dispersion medium, packaging and conjugation services can be discussed.

Are bulk quantities available?

Yes. SHBC supports sample evaluation, pilot orders and bulk production.

Is CSi100UM-10 a finished diagnostic reagent?

No. It is a raw material for research and reagent development. Customers must complete application-specific validation.

Request Samples and Bulk Pricing

Contact SHBC for CSi100UM-10 samples, technical specifications, customization and bulk quotations.

Please provide:

• Required quantity

• Target application

• Target biomolecule

• Preferred coupling method

• Packaging requirements

• Expected annual demand

Product: 100um Carboxyl Silica Microspheres
Catalog Number: CSi100UM-10
Particle Size: 100 µm
Solid Content: 5%
Surface Group: Carboxyl, –COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
Intended Use: Research and Reagent Development Use