100 um Carboxyl Crosslinked PS/DVB Microspheres CPS/DVB100UM 10%

100 μm Carboxyl Crosslinked PS/DVB Microspheres CPS/DVB100UM

SHBC CPS/DVB100UM Carboxyl Crosslinked PS/DVB Microspheres are 100 μm functional polymer microspheres with a crosslinked polystyrene/divinylbenzene matrix and reactive surface carboxyl groups.

Supplied at 10% solids, CPS/DVB100UM is designed for antibody coupling, protein immobilization, bead-based immunoassay development and custom biomolecule-functionalized particle production.

The large 100 μm particle format provides convenient handling, washing and recovery for research and scalable batch processing.

CPS/DVB100UM Technical Specifications

Crosslinked PS/DVB Structure

CPS/DVB100UM uses divinylbenzene to crosslink the polystyrene matrix.

Crosslinked polymer microspheres are used when improved heat, solvent and pressure resistance is required. Bangs also notes that larger polymer spheres can contain DVB to increase structural stability.

Key advantages include:

• Rigid polymer structure

• Stable spherical morphology

• Improved structural stability

• Suitable for repeated washing

• Easy recovery during batch processing

• Suitable for surface functionalization

Carboxyl Surface for Covalent Coupling

Surface carboxyl groups provide reactive sites for covalent attachment of amine-containing biomolecules.

Typical coupling chemistry:

COOH Surface → EDC/NHS Activation → Amine-Containing Ligand → Stable Amide Bond

Carboxyl-functional PS and PS/DVB microspheres are commonly used for EDAC/EDC-mediated immobilization of proteins, peptides, DNA and other amine-containing molecules.

Key Advantages of CPS/DVB100UM

• 100 μm particle size

• High 10% solid content

• Crosslinked PS/DVB matrix

• Reactive carboxyl surface

• Suitable for EDC/NHS chemistry

• Stable covalent ligand attachment

• Easy particle recovery

• Convenient centrifugation and washing

• Suitable for antibodies and antigens

• Suitable for bulk reagent production

Why Choose 100 μm Carboxyl Microspheres?

The 100 μm particle format is suitable for applications where straightforward particle handling and rapid recovery are important.

Potential advantages include:

• Rapid sedimentation

• Easy centrifugation

• Convenient washing

• Easy visual observation

• Reduced particle loss during processing

• Straightforward batch coupling

• Easy resuspension

• Suitable for larger-volume workflows

Compared with smaller microspheres, 100 μm particles provide easier physical handling but lower total surface area per unit mass.

The optimum size should therefore be selected according to required ligand loading, assay kinetics and process design.

Immunoassay Applications

Antibody-Coupled Microspheres

Capture antibodies can be covalently immobilized on CPS/DVB100UM for:

• Bead-based immunoassays

• Antigen detection

• Biomarker detection

• Serological assay development

• Pathogen-detection research

• Custom diagnostic reagent development

Covalent attachment is useful when stable ligand retention is required during repeated washing and storage.

Antigen-Coupled Microspheres

Antigens can be immobilized for:

• Antibody detection

• Serological research

• Autoantibody assay development

• Infectious-disease research

• Assay development

• Method validation

Protein and Biomolecule Applications

CPS/DVB100UM can also be evaluated for:

• Protein immobilization

• Enzyme immobilization

• Peptide coupling

• Affinity ligand preparation

• Biomolecule capture

• Biosensor development

• Surface chemistry research

• Oligonucleotide immobilization

• Custom functional particle preparation

Functionalized polystyrene microspheres are widely used in assays, diagnostics and other applications requiring reactive surface chemistry.

Suitable Coupling Molecules

The carboxyl-functional surface can be evaluated with:

• Monoclonal antibodies

• Polyclonal antibodies

• Recombinant proteins

• Diagnostic antigens

• Peptides

• Enzymes

• Amino-modified DNA

• Amino-modified RNA

• Other amine-containing ligands

Final coupling efficiency depends on ligand purity, concentration, accessible amino groups and reaction conditions.

General EDC/NHS Coupling Workflow

1. Resuspend the Microspheres

Mix CPS/DVB100UM thoroughly before sampling.

Because 100 μm particles settle quickly, resuspend immediately before use.

2. Wash the Microspheres

Wash the particles using a suitable activation buffer.

3. Activate Carboxyl Groups

Add EDC, with or without NHS, to activate surface carboxyl groups.

4. Add the Biomolecule

Add the purified antibody, antigen, protein or other amine-containing ligand.

5. Perform Covalent Coupling

Allow activated carboxyl groups to react with primary amino groups on the biomolecule.

6. Block Residual Reactive Sites

Use a suitable blocking or quenching reagent after coupling.

7. Wash and Resuspend

Remove unbound biomolecules and coupling reagents, then resuspend the functionalized microspheres in the selected storage or assay buffer.

Factors Affecting Coupling Efficiency

Important optimization parameters include:

• Microsphere concentration

• Ligand concentration

• EDC concentration

• NHS concentration

• Activation pH

• Coupling-buffer composition

• Reaction temperature

• Incubation time

• Mixing efficiency

• Blocking conditions

Small-scale optimization is recommended before production scale-up.

Handling Tips for 100 μm Microspheres

Large particles require different handling from nano- or small-micron beads.

Recommended practices include:

• Resuspend before every sampling step.

• Use gentle but sufficient mixing.

• Avoid prolonged settling during coupling.

• Use pipette tips with suitable internal diameter.

• Minimize unnecessary transfer steps.

• Confirm recovery after each wash.

• Avoid excessive mechanical shear.

• Validate suspension uniformity during scale-up.

CPS/DVB100UM vs. Smaller Carboxyl Microspheres

The preferred particle size depends on ligand capacity, process handling and assay design.

Crosslinked PS/DVB vs. Standard PS Microspheres

Bangs specifically describes crosslinked polymer microspheres as offering improved solvent, heat and pressure resistance.

Covalent Coupling vs. Passive Adsorption

Quality Control for Bulk Production

For immunoassay reagent development, recommended evaluation items include:

• Particle-size consistency

• Suspension uniformity

• Solid-content consistency

• Surface carboxyl functionality

• Coupling efficiency

• Ligand loading

• Nonspecific binding

• Recovery after washing

• Assay sensitivity

• Storage stability

• Batch-to-batch reproducibility

Why Choose SHBC CPS/DVB100UM?

• 100 μm carboxyl crosslinked PS/DVB microspheres

• High 10% solid-content suspension

• Reactive COOH surface

• Suitable for EDC/NHS coupling

• Designed for antibody and protein immobilization

• Suitable for bead-based immunoassay development

• Sample evaluation available

• Stable batch manufacturing

• Bulk production capability

• Custom quantity and packaging support

Storage and Handling

Recommended practices include:

• Keep the container tightly sealed.

• Mix thoroughly before sampling.

• Resuspend settled particles before use.

• Avoid unnecessary freezing.

• Prevent microbial contamination.

• Use suitable pipette tips for large particles.

• Avoid conditions causing irreversible aggregation.

• Validate storage conditions after biomolecule coupling.

Frequently Asked Questions

What is CPS/DVB100UM?

CPS/DVB100UM is a 100 μm carboxyl-functional crosslinked polystyrene/divinylbenzene microsphere product supplied at 10% solids.

What does PS/DVB mean?

PS/DVB means polystyrene crosslinked with divinylbenzene.

Why use DVB crosslinking?

DVB creates a more rigid polymer network and improves the structural stability of larger polystyrene microspheres.

What is the function of the carboxyl surface?

Surface carboxyl groups provide reactive sites for covalent attachment of antibodies, proteins and other amine-containing biomolecules.

Can antibodies be coupled to CPS/DVB100UM?

Yes. Antibodies can be immobilized using optimized EDC or EDC/NHS coupling chemistry.

Can CPS/DVB100UM be used for immunoassays?

Yes. It can be evaluated for large-particle bead-based antibody, antigen and biomarker-detection applications.

What is the solid content?

CPS/DVB100UM is supplied at 10% solids.

Can amino-modified oligonucleotides be coupled?

Yes. Amino-modified DNA or RNA can be evaluated for carbodiimide-mediated coupling to the carboxyl surface.

Why choose 100 μm microspheres?

100 μm particles provide very convenient handling, sedimentation and recovery for batch processes. Smaller particles generally provide greater available surface area per unit mass.

Do 100 μm particles require continuous mixing?

Regular mixing is recommended because large particles settle rapidly. The optimum mixing method should be validated for the coupling process.

Can 100 μm microspheres be used in flow cytometry?

Most flow-cytometry applications use substantially smaller particles, so instrument compatibility should be confirmed before use. CPS/DVB100UM is better positioned for large-particle bead assays and biomolecule immobilization unless a specific instrument has been validated.

Is coupling optimization necessary?

Yes. EDC/NHS concentration, activation pH, ligand loading, reaction time and blocking conditions should be optimized for each project.

Can SHBC supply bulk quantities?

Yes. SHBC supports sample evaluation and bulk supply for immunoassay developers, biotechnology companies and research organizations.

Request CPS/DVB100UM Samples

Contact Shanghai SanYu Biotechnology Co., Ltd. for CPS/DVB100UM samples, technical documentation and bulk quotations.

Please provide:

• Target antibody or protein

• Assay format

• Coupling chemistry

• Required microsphere quantity

• Target application

• Development stage

• Estimated annual demand

CPS/DVB100UM is supplied for research and process development. Final diagnostic applications should be validated by the customer.