10um Carboxyl Red-Fluo Microspheres CFR10UM-10

10um Carboxyl Red-Fluo Microspheres CFR10UM-10

SHBC CFR10UM-10 are 10 μm carboxyl-functionalized red fluorescent microspheres developed for flow cytometry, fluorescence microscopy, phagocytosis studies and biomolecule immobilization.

The microspheres feature excitation at 535 nm, emission at 610 nm and 1% solid content. Surface carboxyl groups support covalent coupling with antibodies, antigens, proteins and peptides.

Product Specifications

Batch-specific particle size, particle size distribution, carboxyl content and fluorescence data are available in the Certificate of Analysis.

Red Fluorescence at 535/610nm

CFR10UM-10 produces red fluorescence with excitation at approximately 535 nm and emission centered at approximately 610 nm.

The optical profile is suitable for compatible flow cytometers, fluorescence microscopes, imaging systems and fluorescence readers.

Key Advantages

Uniform 10um Particle Size

Controlled particle sizing supports repeatable fluorescence detection, surface coupling and particle-based analysis.

Strong Signal per Particle

The 10 μm particle body provides a strong fluorescent signal for clear particle identification and imaging.

Reactive Carboxyl Surface

Surface COOH groups support covalent immobilization of antibodies, antigens, proteins, peptides and other amino-containing molecules.

Large Coupling Surface

The 10 μm diameter provides substantial surface area per particle for biomolecule immobilization.

Easy Recovery and Washing

Micrometer-sized particles can be conveniently collected by centrifugation during conjugation and washing procedures.

Custom and Bulk Supply

SHBC supports laboratory samples, customized specifications, pilot production and industrial batch supply.

Main Applications

Flow Cytometry

CFR10UM-10 can be evaluated for particle detection, fluorescence channel testing, gating development and bead-based assays.

Fluorescence Microscopy

The red fluorescent signal supports particle localization, distribution and imaging studies.

Phagocytosis Studies

The 10 μm microspheres can be evaluated in macrophage uptake, phagocytosis and immune-cell interaction research.

Cell–Particle Interaction Studies

Suitable for studying particle adhesion, surface recognition, cellular contact and internalization.

Particle-Based Immunoassays

Antibodies or antigens can be immobilized on the carboxyl surface for suspension immunoassays and agglutination studies.

Biomolecule Immobilization

Suitable for coupling proteins, enzymes, peptides and other molecules containing primary amine groups.

Fluorescent Tracer Studies

Can be used in fluid-flow, filtration, particle transport and selected tissue distribution studies.

Instrument and Method Development

Suitable for fluorescence detection, particle counting, imaging and analytical method development.

Biomolecule Coupling

Surface carboxyl groups can be activated using EDC/NHS chemistry and coupled with primary amine groups on antibodies or proteins.

A typical coupling workflow includes:

1. Wash and redisperse the microspheres.

2. Activate the carboxyl groups with EDC and NHS.

3. Add the antibody or target protein.

4. Block remaining active sites.

5. Wash and resuspend the conjugated particles.

6. Evaluate coupling and fluorescence performance.

Activation pH, reagent concentration, protein loading and reaction time should be optimized for each biomolecule.

Coupling Benefits

CFR10UM-10 provides:

• Reactive surface carboxyl groups

• Large surface area per particle

• Stable covalent immobilization

• Easy centrifugation and washing

• Clear fluorescence detection

• Flexible ligand modification

• Compatibility with bead-based assays

• Custom conjugation support

Flow Cytometry Considerations

Recommended parameters to optimize include:

• Particle concentration

• Excitation source

• Emission filter

• Sample flow rate

• FSC and SSC settings

• Detector voltage

• Gating strategy

• Doublet discrimination

• Particle aggregation

Select a fluorescence channel compatible with emission near 610 nm.

Instrument compatibility should be confirmed using the actual excitation source and filter set.

Cell and Phagocytosis Studies

For cellular experiments, evaluate:

• Particle-to-cell ratio

• Incubation time

• Cell type

• Particle surface modification

• Ligand density

• Culture conditions

• Washing efficiency

• Internalization controls

• Background fluorescence

Include untreated cells, particle-only samples and suitable fluorescence controls.

Dispersion and Handling

Before use:

• Mix by gentle inversion or controlled vortexing

• Redisperse settled particles completely

• Avoid excessive bubbles and foam

• Protect the suspension from strong light

• Prevent the microspheres from drying

• Avoid repeated freeze-thaw cycles

• Confirm buffer compatibility

• Use clean laboratory equipment

Controlled bath sonication may be used to break weak aggregates. Avoid aggressive probe sonication that may damage the particles.

Centrifugation and Washing

The 10 μm particles can normally be collected by low-speed centrifugation.

Centrifugation speed and time should be optimized according to:

• Suspension volume

• Buffer viscosity

• Tube type

• Particle concentration

• Required recovery rate

Avoid excessive centrifugation that may form a difficult-to-redisperse pellet.

Application Limitations

The 10 μm particle size is generally too large for conventional nitrocellulose membrane migration.

For standard fluorescent lateral flow assays, smaller submicron microspheres are normally more suitable.

CFR10UM-10 is better suited to suspension assays, flow cytometry, fluorescence imaging and cell-interaction research.

Custom Carboxyl Fluorescent Microspheres

SHBC can customize fluorescent microspheres according to research and production requirements.

Customization options may include:

• Custom particle size

• Custom fluorescence wavelength

• Different fluorescence colors

• Carboxyl group density

• Solid content

• Dispersion buffer

• Surface modification

• Biomolecule conjugation

• Custom packaging

• OEM and ODM production

Please provide the target application, required wavelength, biomolecule and order quantity when requesting customization.

Quality Control and Documents

Available product documents may include:

• Certificate of Analysis

• Technical Data Sheet

• Safety Data Sheet

• Particle size report

• Particle size distribution data

• Fluorescence spectrum

• Carboxyl content data

• Microscopy images

• Batch inspection report

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. supplies fluorescent microspheres, colored latex particles, magnetic beads and functional microsphere materials.

SHBC provides:

• Controlled microsphere manufacturing

• Stable particle size distribution

• Multiple fluorescence wavelengths

• Functional surface modification

• Batch-to-batch consistency

• Sample evaluation support

• Pilot and bulk production

• OEM and ODM services

Frequently Asked Questions

What is CFR10UM-10?

CFR10UM-10 is a 10 μm carboxyl-functionalized red fluorescent microsphere suspension with 1% solid content.

What are the excitation and emission wavelengths?

The excitation wavelength is 535 nm and the emission wavelength is 610 nm.

What is CFR10UM-10 mainly used for?

It is suitable for flow cytometry, fluorescence microscopy, phagocytosis research, cell–particle interaction and biomolecule coupling.

Can antibodies be coupled to CFR10UM-10?

Yes. Antibodies and proteins containing primary amine groups can be covalently coupled to the carboxyl surface.

Which coupling chemistry is recommended?

EDC/NHS chemistry is commonly used to activate surface carboxyl groups for protein immobilization.

Is CFR10UM-10 suitable for flow cytometry?

Yes. Excitation compatibility, emission filters, scatter settings, particle concentration and gating conditions should be optimized.

Can CFR10UM-10 be used for phagocytosis studies?

Yes. The 10 μm microspheres can be evaluated in macrophage uptake and immune-cell interaction studies.

Is CFR10UM-10 suitable for lateral flow assays?

The particles are generally too large for conventional nitrocellulose membrane migration. Smaller fluorescent microspheres are more suitable for standard LFIA formats.

How should CFR10UM-10 be dispersed?

Use gentle inversion, controlled vortexing or compatible bath sonication while avoiding excessive foam and particle damage.

Can the particles be separated by centrifugation?

Yes. The 10 μm particles can generally be collected by centrifugation. Speed and time should be optimized for the working buffer and volume.

Can CFR10UM-10 be customized?

Yes. Particle size, fluorescence wavelength, carboxyl density, solid content, buffer, surface ligand and packaging can be customized.

Are samples and bulk quantities available?

Yes. SHBC supports laboratory samples, pilot orders and industrial batch production.

Request a Sample or Quotation

Contact SHBC for CFR10UM-10 samples, technical specifications, conjugation support and bulk pricing.

Please provide:

• Target application

• Target biomolecule

• Detection instrument

• Required quantity

• Preferred packaging

• Estimated annual demand

Product: 10um Carboxyl Red-Fluo Microspheres
Catalog Number: CFR10UM-10
Particle Size: 10 μm
Solid Content: 1%
Excitation/Emission: 535/610 nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.