10um Nickel Magnetic Beads MagNi10UM-10 5%

10um Nickel Magnetic Beads MNi10UM-10

SHBC MNi10UM-10 Nickel Magnetic Beads are 10 μm magnetic microspheres with a nickel-affinity surface and 5% solid content.

They are designed for magnetic capture, separation and purification of His-tagged proteins in research and process-development workflows.

Product Overview

MNi10UM-10 combines a 10 μm particle size, nickel affinity and convenient magnetic separation.

The beads provide a solid phase for capturing polyhistidine-tagged recombinant proteins from clarified lysates, protein solutions and other compatible samples.

The concentrated 5% suspension supports flexible dilution, repeated purification experiments and bulk applications.

Technical Specifications

Binding capacity, nickel loading, magnetic response and buffer composition are specification-dependent. Refer to the applicable TDS and COA.

Key Advantages

• Nominal particle size of 10 μm

• Concentrated 5% solids

• Nickel-affinity surface

• Convenient magnetic collection

• No centrifugation required for bead separation

• Suitable for His-tagged protein capture

• Easy washing and buffer exchange

• Suitable for manual workflow development

• Suitable for multiwell plate evaluation

• Flexible packaging and bulk supply

How Do Nickel Magnetic Beads Work?

Nickel-affinity purification is based on immobilized metal affinity chromatography, commonly called IMAC.

Histidine residues in a His tag interact with immobilized nickel ions on the bead surface. A magnet then collects the beads while unbound sample components are removed.

After washing, the captured protein can be eluted using a validated buffer system.

Applications of 10um Nickel Magnetic Beads

His-Tagged Protein Purification

MNi10UM-10 is suitable for capturing and purifying recombinant proteins containing accessible polyhistidine tags.

Recombinant Protein Screening

The beads can support rapid screening of His-tagged protein expression from multiple samples.

Magnetic Protein Separation

A compatible magnetic separator enables convenient bead collection, washing and liquid removal.

Protein Pull-Down Assays

His-tagged bait proteins may be immobilized on the beads for protein-interaction and pull-down research.

His-Tagged Protein Immobilization

MNi10UM-10 can support immobilization of His-tagged proteins for downstream binding and analytical studies.

High-Throughput Workflow Development

The beads may be evaluated in tubes, multiwell plates and automated magnetic-separation systems.

Native and Denaturing Purification

Nickel-affinity purification can be developed under native or selected denaturing conditions, depending on the bead formulation and target protein.

Benefits of 10um Particle Size

The 10 μm particle format provides convenient handling during magnetic collection, washing and resuspension.

Typical formats include:

• Microcentrifuge tubes

• Magnetic separation racks

• Multiwell plates

• Automated liquid-handling systems

• Small-scale protein purification

• Process optimization experiments

Benefits of 5% Solid Content

MNi10UM-10 is supplied at 5% solids as a concentrated magnetic bead suspension.

Users can adjust the bead amount according to sample volume, target concentration and purification requirements.

The concentrated format supports repeated experiments, pilot projects and larger-volume production needs.

Typical His-Tag Purification Workflow

1. Resuspend the Beads

Mix the suspension thoroughly before sampling to obtain a uniform bead concentration.

2. Wash and Equilibrate

Collect the beads with a magnet, remove the storage solution and equilibrate them with a compatible binding buffer.

3. Add the Sample

Add clarified lysate or protein solution and mix gently to promote interaction between the His tag and nickel surface.

4. Perform Magnetic Separation

Collect the beads with a compatible magnet and remove the unbound fraction.

5. Wash the Beads

Wash the beads to reduce nonspecific proteins and other sample components.

6. Elute the Target Protein

Elute the captured protein using an optimized imidazole, pH-based or other compatible elution method.

Conditions should be optimized for each target protein and sample matrix.

Buffer Compatibility and Optimization

Nickel-affinity performance may be affected by:

• Buffer pH

• Salt concentration

• Imidazole concentration

• Detergents

• Reducing agents

• Denaturants

• Chelating agents

• Sample viscosity

• Incubation time

• His-tag accessibility

Strong chelating agents may remove nickel ions and reduce binding performance. Compatibility should be confirmed before use.

Factors Affecting Protein Recovery

Protein recovery and purity depend on:

• Position of the His tag

• Accessibility of the His tag

• Target protein concentration

• Bead-to-sample ratio

• Binding time

• Mixing efficiency

• Wash stringency

• Elution conditions

• Competing proteins

• Sample composition

Small-scale optimization is recommended before pilot or batch processing.

Quality and Batch Documentation

Available documentation may include:

• Product code

• Batch number

• Nominal particle size

• Solid content

• Product appearance

• Surface type

• Certificate of Analysis

• Safety Data Sheet

• Technical Data Sheet

Binding capacity and magnetic response should only be stated according to the applicable SHBC specification.

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. supplies magnetic beads and functional microspheres for biomolecule separation, protein purification and assay development.

SHBC supply advantages include:

• Multiple magnetic bead sizes

• Nickel and other functional surfaces

• Laboratory sample supply

• Pilot-scale manufacturing

• Bulk production capability

• Customized solid content

• Flexible packaging volumes

• OEM and private-label services

• Technical product-selection support

Customized Nickel Magnetic Beads

Customization options may include:

• Particle diameter

• Particle-size distribution

• Solid content

• Nickel-affinity surface

• Chelating ligand

• Dispersion medium

• Buffer system

• Packaging volume

• OEM labeling

• Bulk production

Storage and Handling

Store MNi10UM-10 according to the product label, TDS, COA and SDS.

Thoroughly resuspend the beads before sampling. Avoid allowing the beads to dry during washing or purification.

Use clean laboratory tools and a compatible magnetic separator. Do not freeze unless freezing compatibility is confirmed in the product specification.

Frequently Asked Questions

What is MNi10UM-10?

MNi10UM-10 is a 10 μm nickel-affinity magnetic bead suspension with a solid content of 5%.

What are nickel magnetic beads used for?

They are mainly used for magnetic capture, separation, purification and immobilization of His-tagged proteins.

What is a His-tagged protein?

A His-tagged protein contains a short sequence of histidine residues that enables affinity binding to immobilized nickel ions.

What is IMAC?

IMAC means immobilized metal affinity chromatography. It uses immobilized metal ions to capture proteins containing accessible histidine residues.

Can MNi10UM-10 purify 6×His-tagged proteins?

The beads are designed for polyhistidine-tagged protein capture. Performance depends on tag accessibility, sample composition and purification conditions.

Is centrifugation required?

A compatible magnet can be used to collect the beads, so centrifugation is generally not required for bead separation.

Can imidazole be used?

Imidazole is commonly evaluated during binding, washing and elution. The appropriate concentration depends on the target protein and sample.

Can EDTA be used?

Strong chelating agents such as EDTA may remove nickel ions from the surface and reduce binding performance.

Can the beads be used under denaturing conditions?

Selected denaturing conditions may be evaluated after compatibility with the bead formulation has been confirmed.

What is the binding capacity?

Binding capacity depends on the surface chemistry, target protein and purification conditions. Refer to the applicable SHBC technical specification.

Can MNi10UM-10 be used in automated systems?

The beads may be evaluated in multiwell plate and automated magnetic-separation workflows.

Are bulk quantities available?

Yes. SHBC supports laboratory samples, pilot projects, customized packaging and bulk production.

Request MNi10UM-10 Samples and Quotations

Contact Shanghai SanYu Biotechnology Co., Ltd. for MNi10UM-10 samples, technical specifications, customized packaging and bulk quotations.

SHBC supplies nickel magnetic beads for His-tagged protein purification, affinity capture, protein immobilization and research applications.

For Research Use Only.