Products
SHBC provides colored microspheres, fluorescent microspheres, magnetic beads, silica microspheres, chromatography packing microspheres and biological reagents for diagnostic assay development, nucleic acid extraction, protein purification and separation applications.
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MagNi1UM-10
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SHBC
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5%
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1µm
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10ml,20ml,50ml ,500ml,1000ml
1µm Nickel Magnetic Beads for Immunoassay Research
SHBC MagNi1UM-10 Nickel Magnetic Beads are 1µm magnetic particles with a nickel-functional surface.
They are developed for the capture, purification and immobilization of His-tagged proteins.
The product is supplied at 5% solids for immunoassay research, recombinant antigen preparation, magnetic affinity capture and bulk reagent production.
Product: 1µm Nickel Magnetic Beads
Catalog Number: MagNi1UM-10
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
Particle Size: 1µm
Surface: Nickel functionalized
Solids Content: 5%
Main Target: His-tagged proteins
Main Application: Immunoassay research
Supply: Samples, pilot batches and bulk production
Intended Use: Research use only
Product Overview
MagNi1UM-10 provides a magnetic affinity surface for His-tagged biomolecules.
Nickel ions interact with exposed histidine residues in a polyhistidine tag.
The captured protein can then be:
Magnetically separated
Washed
Concentrated
Used on the beads
Eluted for downstream use
Nickel affinity magnetic beads are commonly used for manual and automated purification of polyhistidine-tagged proteins.
The 1µm particle size provides:
High particle numbers
Large collective surface area
Good suspension contact
Efficient target interaction
Suitability for small reaction volumes
Potential automation compatibility
MagNi1UM-10 can be used to prepare His-tagged antigens or capture proteins for magnetic immunoassay development.
Technical Specifications
Parameter | Specification |
|---|---|
Product name | Nickel Magnetic Beads |
Catalog number | MagNi1UM-10 |
Brand | SHBC |
Manufacturer | Shanghai SanYu Biotechnology Co., Ltd. |
Nominal diameter | 1µm |
Surface | Nickel functionalized |
Solids content | 5% |
Separation method | External magnetic field |
Main binding target | His-tagged proteins |
Main application | Immunoassay research |
Supply format | Samples and bulk supply |
Intended use | Research use only |
The following values should be confirmed by the final TDS or lot-specific COA:
Particle-size range
Particle-size CV
Particle morphology
Magnetic material
Magnetic content
Magnetic response time
Nickel loading
Nickel leakage
Chelating ligand type
His-tag protein binding capacity
Particle concentration
Suspension buffer
Preservative
Package size
Shelf life
Storage temperature
The exact chelating chemistry should be stated in the final specification.
Possible nickel-chelating surfaces include NTA, IDA and other chelators. Their binding strength and reagent compatibility may differ.
The 5% solids content alone cannot determine protein-binding capacity or magnetic response time.
Key Advantages
Fast His-Tag Protein Capture
The nickel-functional surface binds exposed histidine residues in His-tagged proteins.
This supports rapid protein capture from compatible samples.
No Antibody Biotinylation Required
A His-tagged protein can bind through its affinity tag.
Biotinylation and streptavidin are not required.
No EDC/NHS Coupling Required
His-tag binding is based on metal-affinity coordination.
The user does not normally need to activate carboxyl groups or perform covalent protein coupling.
Reversible Affinity Binding
His-tagged proteins can often be released using a competitive elution condition.
Imidazole is commonly used to compete with histidine residues for nickel-binding sites.
1µm Particle Size
The 1µm diameter provides many magnetic particles per unit mass.
This creates numerous target-capture surfaces.
Magnetic Separation
An external magnet collects the particles.
This supports:
Fast washing
Buffer exchange
Sample cleanup
Target concentration
Automated processing
Parallel sample handling
Immunoassay Development
His-tagged recombinant antigens can be immobilized on the beads.
The prepared beads can be evaluated in antibody-detection and protein-binding assays.
Automation Potential
MagNi1UM-10 can be evaluated in:
Manual magnetic workflows
Semi-automated systems
Automated magnetic processors
Multiwell-plate workflows
High-throughput screening
Thermo Fisher’s 1µm Ni-NTA magnetic beads similarly support both magnetic-stand and automated-platform workflows.
5% Solids Suspension
The concentrated format supports:
Laboratory testing
Process optimization
Pilot manufacturing
Repeated reagent preparation
Enterprise bulk production
Bulk Manufacturing
SHBC supports:
Research samples
Pilot batches
Repeat orders
OEM manufacturing
Private-label packaging
Enterprise bulk supply
How Nickel–His Tag Binding Works
Nickel magnetic beads contain immobilized nickel ions held by a chelating surface.
The nickel ions coordinate with histidine residues in a polyhistidine tag.
The simplified binding structure is:
Magnetic Bead – Chelator – Ni²⁺ – His-Tagged Protein
A common affinity tag contains six histidine residues and is called a 6×His tag.
The tag may be located at the N-terminus or C-terminus of a recombinant protein.
Binding
His-tagged protein is mixed with equilibrated nickel magnetic beads.
The histidine tag binds to available nickel sites.
Washing
A low concentration of imidazole may help reduce non-specific binding.
The exact concentration must be optimized for the target protein.
Elution
A higher imidazole concentration can compete with the His tag and release the captured protein.
A change in pH may also be evaluated when compatible with the protein and bead chemistry.
On-Bead Use
Elution is not always required.
His-tagged proteins may remain on the magnetic beads for:
Immunoassays
Antibody screening
Protein-interaction studies
Target capture
Biosensor research
Immunoassay Applications
His-Tagged Antigen Immobilization
A recombinant antigen with a His tag can be captured on MagNi1UM-10.
The antigen-loaded beads can be used for antibody-detection research.
Potential applications include:
Serological assay development
Antibody screening
Hybridoma screening
Vaccine-response research
Autoantibody research
Infectious-disease research
His-Tagged Capture Protein Immobilization
His-tagged receptors, antigens or binding proteins may be loaded onto the magnetic surface.
The prepared beads can capture a target analyte from the sample.
Chemiluminescent Immunoassay Research
MagNi1UM-10 may be evaluated as a magnetic solid phase in CLIA research.
A possible workflow is:
Load a His-tagged capture protein.
Add the test sample.
Capture the target.
Magnetically wash the beads.
Add a labeled detection reagent.
Perform final washing.
Add the chemiluminescent substrate.
Measure the signal.
The complete assay must be validated for ligand retention, background and signal stability.
Fluorescent Immunoassays
The beads may be combined with fluorescent detection antibodies or probes.
Enzyme Immunoassays
The magnetic solid phase may be used with enzyme-labeled detection reagents.
Antibody Screening
His-tagged antigens can be immobilized for:
Monoclonal antibody screening
Polyclonal antibody testing
Antibody specificity studies
Cross-reactivity evaluation
Binding-affinity comparison
Recombinant Protein Purification
MagNi1UM-10 may be evaluated for purification of His-tagged proteins from:
Bacterial lysates
Yeast expression samples
Mammalian expression samples
Insect-cell expression samples
Cell-free expression systems
Compatibility depends on the sample, buffer and final bead chemistry.
Protein Interaction Research
His-tagged bait proteins may be immobilized for:
Protein–protein interaction studies
Ligand screening
Receptor-binding research
Pull-down research
Affinity capture
Suitable Target Molecules
MagNi1UM-10 may be evaluated with:
6×His-tagged proteins
Polyhistidine-tagged proteins
His-tagged recombinant antigens
His-tagged antibodies
His-tagged antibody fragments
His-tagged enzymes
His-tagged receptors
His-tagged peptides
Histidine-rich affinity ligands
The His tag must remain accessible after protein folding.
A hidden or sterically blocked His tag may reduce binding.
Important Limitation
Standard antibodies without a His tag should not be assumed to bind specifically or directionally.
For ordinary antibodies, consider:
Carboxyl magnetic beads
NHS magnetic beads
Epoxy magnetic beads
Tosyl magnetic beads
Protein A/G magnetic beads
Streptavidin magnetic beads with biotinylated antibodies
Recommended His-Tagged Protein Binding Workflow
The following is a general development workflow.
Final conditions should follow the SHBC TDS and lot-specific COA.
1. Resuspend the Beads
Mix MagNi1UM-10 until homogeneous.
Use gentle inversion or controlled vortexing.
Avoid excessive foam.
2. Transfer the Required Amount
Calculate the bead quantity according to:
Protein amount
Required binding capacity
Number of tests
Reaction volume
Expected process loss
3. Magnetically Collect the Beads
Place the tube in a compatible magnetic separator.
Wait until the beads have collected.
Remove the storage solution.
4. Equilibrate the Beads
Wash the beads with a compatible binding buffer.
The buffer may contain:
Phosphate or another compatible buffer
Sodium chloride
Low imidazole
A compatible nonionic detergent
Final composition must be optimized.
5. Prepare the Sample
Clarify the protein sample when necessary.
Check:
His-tag accessibility
Protein solubility
Sample pH
Salt concentration
Imidazole concentration
Chelator content
Reducing-agent content
Detergent compatibility
6. Add the Sample
Combine the prepared sample with the equilibrated beads.
Mix gently to maintain uniform suspension.
7. Incubate
Optimize:
Binding time
Temperature
Mixing speed
Bead-to-protein ratio
Buffer pH
Salt concentration
Imidazole concentration
8. Magnetically Wash
Collect the beads with a magnet.
Remove unbound material.
Repeat washing as required.
9. Use or Elute the Protein
The protein-loaded beads may be used directly.
Alternatively, elute the His-tagged protein with a validated competitive buffer.
10. Evaluate Performance
Measure:
Protein recovery
Protein purity
Binding capacity
Non-specific binding
Ligand activity
Magnetic recovery
Bead aggregation
Magnetic Immunoassay Workflow
Load the His-tagged antigen or capture protein.
Wash the magnetic beads.
Block the remaining surface when required.
Add the test sample.
Incubate for target binding.
Magnetically collect the beads.
Wash away unbound sample.
Add the labeled detection reagent.
Incubate again.
Perform final magnetic washing.
Add the detection substrate.
Measure the signal.
Possible detection systems include:
Chemiluminescence
Electrochemiluminescence
Fluorescence
Enzyme colorimetry
Other compatible reporters
Why Choose 1µm Magnetic Beads?
High Particle Number
A 1µm suspension contains more individual particles per unit mass than larger magnetic beads of similar density.
This provides many target-capture surfaces.
Large Collective Surface Area
Smaller particles generally provide a larger collective surface area per unit mass than larger particles.
This may support rapid protein interaction.
Good Suspension Contact
The particles can remain dispersed during controlled mixing.
This increases contact between the bead surface and the target protein.
Suitable for Small Volumes
The beads may be evaluated in microtube and multiwell-plate formats.
Automation Positioning
The 1µm size is used by established suppliers in automated His-tag protein purification platforms.
Important Trade-Off
Compared with larger beads, 1µm particles may require:
A suitable high-gradient magnet
Longer collection in some vessels
Careful aspiration
Validated redispersion
Good aggregate control
Actual performance depends on magnetic content and magnet design.
Nickel vs NHS and Streptavidin Magnetic Beads
Surface | Binding Target | Main Advantage | Main Limitation |
|---|---|---|---|
Nickel | His-tagged proteins | Fast reversible affinity capture | Target normally needs an accessible His tag |
NHS | Primary amines | Direct covalent protein coupling | Activated surface is sensitive to hydrolysis |
Carboxyl | Primary amines after EDC/NHS activation | Flexible covalent coupling | Requires activation |
Streptavidin | Biotinylated molecules | Fast modular loading | Target must be biotinylated |
Protein A/G | Antibody Fc region | Direct antibody binding | Species and isotype compatibility vary |
Choose nickel magnetic beads when:
The target protein has a His tag.
Reversible binding is useful.
Recombinant antigen immobilization is required.
Fast affinity purification is needed.
His-tagged protein screening is planned.
Choose NHS or carboxyl beads when:
A non-His-tagged protein must be covalently immobilized.
Ligand leakage must be minimized.
Permanent conjugation is preferred.
Choose streptavidin beads when:
The ligand is biotinylated.
Rapid modular loading is required.
Quality Control
Recommended quality-control items include:
Mean particle diameter
Particle-size range
Particle-size CV
Particle morphology
Suspension appearance
Solids content
Magnetic content
Magnetic response
Magnetic recovery
Redispersion
Aggregate level
Nickel loading
Nickel leakage
His-tag protein binding capacity
Non-specific binding
Functional antigen activity
Batch consistency
Protein-Binding Capacity
Binding capacity should be measured using a defined His-tagged protein.
The result depends on:
Protein size
His-tag position
Tag accessibility
Protein purity
Buffer conditions
Incubation time
Test method
Do not estimate binding capacity from solids content alone.
Magnetic Performance
Evaluate:
Magnetic collection time
Percentage bead recovery
Residual beads in the supernatant
Redispersion after collection
Performance in the intended vessel
Compatibility with the intended analyzer
Functional Immunoassay Testing
A model assay may evaluate:
Positive signal
Negative background
Signal-to-background ratio
Ligand retention
Dose-response performance
Precision
Wash efficiency
Lot-to-lot consistency
Bulk Manufacturing and Customization
Shanghai SanYu Biotechnology Co., Ltd. supports:
Research samples
Pilot batches
Bulk manufacturing
OEM production
Private-label packaging
Custom package sizes
Custom solids concentration
Custom nickel loading
Custom magnetic response
Customer-specific quality standards
For project evaluation, provide:
Required quantity
Annual demand
Intended application
His-tagged protein type
Required binding capacity
Bead amount per test
Magnetic separator or analyzer
Preferred package size
Buffer restrictions
Preservative restrictions
Quality-control requirements
Handling and Storage
Follow the final SHBC product label, TDS and lot-specific COA.
General recommendations:
Store under the specified conditions.
Do not freeze unless validated.
Keep the container tightly closed.
Mix before sampling.
Do not allow the beads to dry.
Use clean, low-binding tubes.
Maintain gentle mixing during binding.
Do not return used material to the original bottle.
Protect the product from microbial contamination.
Buffer Compatibility
Nickel-affinity performance can be affected by:
EDTA
Other strong chelators
High imidazole concentrations
Extreme pH
Certain reducing agents
Compatibility depends on the chelating ligand used on MagNi1UM-10.
Some Ni-NTA systems advise avoiding EDTA and strong reducing agents, while other nickel-chelate products are designed with greater resistance. Product-specific compatibility must therefore be confirmed by SHBC testing.
Avoid Drying
Do not allow the magnetic bead pellet to dry.
Drying may cause:
Aggregation
Poor redispersion
Reduced binding
Lower magnetic recovery
Higher assay variation
Frequently Asked Questions
What is MagNi1UM-10?
MagNi1UM-10 is a 1µm nickel-functional magnetic bead suspension supplied at 5% solids.
What is its main binding target?
It is designed primarily for His-tagged proteins.
What is a His tag?
A His tag is a short sequence containing multiple histidine residues.
A common format is the 6×His tag.
Can ordinary antibodies bind directly?
Ordinary antibodies should not be assumed to bind specifically.
The antibody should carry an accessible His tag or another compatible histidine-rich affinity sequence.
Can His-tagged antigens be immobilized?
Yes.
His-tagged recombinant antigens can be captured for immunoassay and antibody-screening research.
Is the binding covalent?
Nickel–His tag binding is an affinity interaction based on metal coordination.
It is not the same as permanent covalent coupling.
Can the protein be eluted?
His-tagged protein may be eluted using a validated competitive condition, commonly involving imidazole.
Can the protein-loaded beads be used directly?
Yes.
The loaded beads may be evaluated directly in immunoassays, target capture and protein-interaction studies.
Can MagNi1UM-10 be used in CLIA research?
Yes.
His-tagged antigens or capture proteins may be loaded onto the beads for magnetic CLIA development.
Can it be used for protein purification?
Yes.
It may be evaluated for magnetic purification and screening of His-tagged proteins.
Can it be used on automated instruments?
Yes, subject to validation of dispensing, magnetic collection, washing and redispersion.
What is the protein-binding capacity?
Use the final SHBC product specification or lot-specific COA.
Do not estimate it from the 5% solids content.
Does the product use NTA?
The exact nickel-chelating ligand should be confirmed in the final TDS.
Do not describe the product as Ni-NTA unless NTA is confirmed.
Can EDTA be used?
EDTA may compete for nickel ions in many nickel-affinity systems.
Compatibility must be confirmed for MagNi1UM-10.
Can the beads be frozen?
Freezing is generally not recommended unless validated.
Can the bead pellet be allowed to dry?
No.
Drying may cause aggregation and poor redispersion.
Is bulk production available?
Yes.
SHBC supports samples, pilot batches, OEM projects and enterprise bulk manufacturing.
Request a Sample or Quotation
SHBC MagNi1UM-10 provides:
1µm nominal particle size
Nickel-functional surface
5% solids content
His-tag protein capture
Magnetic separation
Recombinant antigen immobilization
Immunoassay research compatibility
Automation-development potential
Sample and pilot-batch supply
Enterprise bulk production
OEM and customization support
Product Name: 1µm Nickel Magnetic Beads
Catalog Number: MagNi1UM-10
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
Particle Size: 1µm
Surface: Nickel functionalized
Solids Content: 5%
Primary Target: His-tagged proteins
Application: Immunoassay and protein-affinity research
Supply: Samples, pilot batches and bulk production
Intended Use: Research Use Only. Not for diagnostic or therapeutic use.