2-CTC Resin CAS42074-68-0 100-200mesh,1% DVB

2-CTC Resin for Fmoc Solid-Phase Peptide Synthesis

SHBC 2-Chlorotrityl Chloride Resin is a 100–200 mesh, 1% DVB-crosslinked polystyrene support for Fmoc solid-phase peptide synthesis (SPPS).

Its acid-labile linker enables peptides to be released under mild acidic conditions. This makes the resin especially useful for synthesizing peptide acids, protected peptide fragments and acid-sensitive sequences.

For immunoassay developers, 2-CTC Resin serves as an upstream synthesis carrier for producing antigenic peptides, epitope peptides, conjugation-ready peptides and reference peptides. It is not added directly to the immunoassay system.

Key Specifications

Why Choose SHBC 2-CTC Resin?

Mild Cleavage of Protected Peptide Fragments

The acid-labile 2-chlorotrityl linker allows peptide cleavage under relatively mild acidic conditions. Side-chain protecting groups such as Boc and tBu can remain intact when an appropriate cleavage method is used.

Reduced Risk of Racemization

2-CTC Resin can help minimize racemization during attachment of the first amino acid. This is particularly useful for sequences containing sensitive C-terminal residues such as cysteine or histidine.

Lower Diketopiperazine Formation

The steric environment of the 2-chlorotrityl linker helps suppress diketopiperazine formation, an important consideration when synthesizing peptides with C-terminal proline.

Practical Bead Size for Batch Processing

The 100–200 mesh particle size supports efficient solvent contact, filtration and washing during manual, automated and batch peptide synthesis workflows.

1% DVB Crosslinked Polystyrene Matrix

The 1% DVB-crosslinked polystyrene structure is designed to provide suitable swelling and reagent accessibility in commonly used SPPS solvent systems.

2-CTC Resin for Immunoassay Peptide Production

SHBC 2-CTC Resin can be used to synthesize peptides required in immunoassay research and reagent development, including:

• Antigenic peptides for antibody development

• Epitope peptides for antibody specificity studies

• Peptides for competitive immunoassays

• Linker-modified peptides for carrier protein conjugation

• Biotinylated, fluorescent or label-ready peptide precursors

• Reference peptides and peptide controls

• Protected peptide fragments for convergent synthesis

• Peptide libraries for biomarker and drug discovery research

The resin is used during peptide manufacturing. The purified peptide produced from the resin may subsequently be applied in ELISA, lateral flow assays, chemiluminescent immunoassays and other analytical workflows.

How 2-Chlorotrityl Chloride Resin Works

The first Fmoc-protected amino acid is attached to the reactive 2-chlorotrityl chloride group under dry conditions. The peptide chain is then assembled through repeated Fmoc deprotection, amino acid coupling and washing cycles.

After synthesis, the peptide can be cleaved from the resin under selected mild acidic conditions. Depending on the cleavage system, a protected peptide fragment or a fully deprotected peptide may be obtained.

A typical workflow includes:

1. Pre-swell the resin in a compatible dry solvent.

2. Load the first Fmoc-protected amino acid.

3. Cap unreacted functional sites.

4. Perform repeated deprotection and coupling cycles.

5. Wash the resin between reaction steps.

6. Cleave the target peptide using a validated method.

7. Purify and characterize the resulting peptide.

Process conditions should be optimized according to the peptide sequence, loading target and production scale.

Quality Considerations for Batch Peptide Synthesis

For research companies planning batch production, the following parameters should be confirmed during resin qualification:

• Functional loading or substitution level

• Particle-size distribution

• DVB crosslinking percentage

• Moisture content

• Resin swelling performance

• Coupling and cleavage efficiency

• Lot-to-lot consistency

• Required COA, SDS and technical documentation

Pilot-scale evaluation is recommended before transferring a peptide synthesis process to larger batches.

Handling and Storage

2-CTC Resin is sensitive to moisture and acidic contamination. Use dry glassware, dry solvents and anhydrous reaction conditions whenever possible.

Keep the container tightly sealed and store the resin in a cool, dry environment according to the product label and SDS. Avoid prolonged exposure to moisture, acids and unsuitable protic solvents.

Frequently Asked Questions

What is 2-CTC Resin used for?

2-CTC Resin is mainly used as an acid-labile solid support for Fmoc solid-phase peptide synthesis. It is suitable for preparing peptide acids and protected peptide fragments.

Is 2-CTC Resin suitable for immunoassay development?

Yes. It can be used to manufacture antigenic, epitope, conjugation-ready and reference peptides for immunoassay research. The resin itself is not used directly as an immunoassay particle.

What is the difference between 2-CTC Resin and Wang Resin?

2-CTC Resin generally permits milder peptide cleavage than Wang Resin. It is therefore preferred when protected peptide fragments or acid-sensitive sequences are required.

Can 2-CTC Resin produce C-terminal peptide amides?

2-CTC Resin is primarily selected for peptide acids. A Rink amide resin is usually more appropriate when a C-terminal peptide amide is required.

Why is 1% DVB used?

DVB crosslinks the polystyrene matrix. A 1% crosslinking level provides the resin structure while allowing it to swell in suitable peptide synthesis solvents.

What information is needed for a bulk quotation?

Please provide the required quantity, target loading range, peptide synthesis scale, packaging requirement, application and required quality documents.

Request a Sample or Bulk Quote

SHBC supplies 2-CTC Resin for peptide research, process development and batch peptide synthesis.

Contact Shanghai SanYu Biotechnology Co., Ltd. with your required quantity, target loading, packaging and documentation requirements. Evaluation quantities and bulk supply options can be discussed according to your project.