Product Overview
CFR300-10 Carboxyl Red Fluorescent Microspheres are high-performance fluorescent polystyrene nanoparticles designed for fluorescence detection and biomolecule immobilization. Featuring bright red fluorescence, excellent particle size uniformity and reactive surface carboxyl groups, they provide reliable performance in diagnostic assays and life science research.
Supplied as a 1% aqueous suspension, CFR300-10 offers stable fluorescence, excellent colloidal stability and reproducible performance. The surface carboxyl groups can be activated using EDC/NHS coupling chemistry, allowing efficient covalent attachment of antibodies, proteins, peptides and other biomolecules for fluorescence-based assays.
Key Features
Uniform 300 nm particle size
Bright red fluorescence
Surface carboxyl functional groups
High fluorescence intensity
Excellent particle uniformity
Stable aqueous suspension
Compatible with EDC/NHS coupling
High biomolecule conjugation efficiency
Low nonspecific adsorption
Excellent batch-to-batch consistency
Technical Specifications
Parameter | Specification |
|---|
Product Code | CFR300-10 |
Material | Fluorescent Polystyrene Microspheres |
Particle Size | 300 nm |
Surface | Carboxyl (-COOH) |
Solid Content | 1% Suspension |
Excitation (Ex) | 530 nm |
Emission (Em) | 580 nm |
Appearance | Red Fluorescent Suspension |
Storage | 2–8°C |
Packaging | 10 mL / 20 mL / 100 mL |
Applications
Lateral Flow Assays (LFA)
Fluorescence Immunoassays
Chemiluminescence Immunoassays (CLIA)
Antibody Conjugation
Protein Labeling
Cell Labeling
Biosensors
Molecular Diagnostics
Fluorescence Calibration
Life Science Research
Product Advantages
CFR300-10 offers bright and stable red fluorescence combined with highly reactive surface carboxyl groups for efficient biomolecule conjugation. Compared with smaller nanoparticles, the 300 nm particle size provides stronger fluorescence signals and higher antibody loading capacity while maintaining excellent dispersion stability. These properties make CFR300-10 an excellent choice for fluorescence immunoassays, lateral flow assays, biosensors, cell labeling and diagnostic reagent development.
Storage and Handling
Store at 2–8°C.
Do not freeze.
Protect from direct light.
Keep the container tightly sealed.
Gently invert before use.
Avoid repeated freeze-thaw cycles.
Prevent microbial contamination during handling.
Customization Service
SANYU GROUP provides customized fluorescent microspheres including:
Customized particle sizes
Customized fluorescence intensity
Customized excitation and emission wavelengths
Surface functionalization
Customized solid content
OEM manufacturing
Bulk production
Custom packaging
Frequently Asked Questions
What are Carboxyl Red Fluorescent Microspheres?
Carboxyl Red Fluorescent Microspheres are fluorescent polystyrene nanoparticles with reactive carboxyl functional groups for fluorescence detection, biomolecule conjugation and diagnostic assay development.
What are the excitation and emission wavelengths of CFR300-10?
CFR300-10 has an excitation wavelength of 530 nm and an emission wavelength of 580 nm, producing bright red fluorescence for analytical and diagnostic applications.
What applications is CFR300-10 suitable for?
CFR300-10 is widely used for lateral flow assays, fluorescence immunoassays, antibody conjugation, protein labeling, cell labeling, biosensors, molecular diagnostics, fluorescence calibration and life science research.
Can antibodies be conjugated to CFR300-10?
Yes. The surface carboxyl groups can be activated using EDC/NHS chemistry for stable covalent attachment of antibodies, proteins, peptides and other amine-containing biomolecules.
How should CFR300-10 be stored?
Store at 2–8°C, protect the suspension from light, avoid freezing, and gently mix before use to maintain optimal fluorescence performance and particle dispersion.