30um Carboxyl Crosslinked PS/DVB Microspheres CPS/DVB30UM 10%

30 μm Carboxyl Crosslinked PS/DVB Microspheres CPS/DVB30UM

SHBC CPS/DVB30UM Carboxyl Crosslinked PS/DVB Microspheres are 30 μm functional polymer microspheres with a crosslinked polystyrene/divinylbenzene matrix and reactive carboxyl groups.

Supplied at 10% solids, CPS/DVB30UM is designed for antibody coupling, protein immobilization, bead-based immunoassays and custom biomolecule-functionalized particle development.

The larger 30 μm particle format provides convenient handling, washing and recovery for research and scalable reagent production.

CPS/DVB30UM Technical Specifications

Crosslinked PS/DVB Structure

CPS/DVB30UM uses divinylbenzene to crosslink the polystyrene matrix.

The crosslinked polymer network provides increased particle rigidity and improved resistance to heat and compatible solvents compared with non-crosslinked polystyrene.

This makes PS/DVB microspheres suitable for applications requiring repeated washing, surface modification and stable particle morphology.

Carboxyl Surface for Covalent Coupling

Surface carboxyl groups provide reactive sites for covalent attachment of amine-containing biomolecules.

Typical coupling chemistry:

COOH Surface → EDC/NHS Activation → Amine-Containing Ligand → Stable Amide Bond

Suitable ligands may include:

• Monoclonal antibodies

• Polyclonal antibodies

• Antigens

• Recombinant proteins

• Peptides

• Enzymes

• Amino-modified DNA

• Amino-modified RNA

• Other amine-containing biomolecules

Key Advantages of CPS/DVB30UM

• 30 μm particle size

• High 10% solid content

• Crosslinked PS/DVB polymer matrix

• Reactive carboxyl surface

• Suitable for EDC/NHS coupling

• Stable covalent biomolecule immobilization

• Convenient particle washing

• Easy recovery and resuspension

• Suitable for antibody and antigen coupling

• Suitable for research and bulk production

Why Choose 30 μm Carboxyl Microspheres?

The 30 μm particle size is suitable for applications that prioritize straightforward particle handling and larger functional solid phases.

Potential advantages include:

• Easy centrifugation

• Convenient washing

• Easy particle recovery

• Clear sedimentation behavior

• Straightforward resuspension

• Reduced particle loss during processing

• Suitable for batch coupling

• Convenient laboratory and production handling

Compared with smaller microspheres, 30 μm particles generally provide easier physical handling, while smaller particles provide greater surface area per unit mass.

The optimum size should be selected according to binding capacity, assay kinetics and detection method.

Immunoassay Applications

Antibody-Coupled Microspheres

Capture antibodies can be immobilized on CPS/DVB30UM for:

• Bead-based immunoassays

• Antigen detection

• Biomarker detection

• Serological assay development

• Pathogen-detection research

• Custom diagnostic reagent development

Covalent coupling helps retain antibodies on the microsphere surface during repeated washing and storage.

Antigen-Coupled Microspheres

Antigens can be immobilized for:

• Antibody detection

• Serological research

• Autoantibody assays

• Infectious-disease research

• Assay development

• Method validation

Protein and Biomolecule Applications

CPS/DVB30UM can also be evaluated for:

• Protein immobilization

• Enzyme immobilization

• Peptide coupling

• Affinity ligand preparation

• Biomolecule capture

• Biosensor development

• Surface chemistry research

• Oligonucleotide immobilization

• Custom functional particle preparation

Suitable Biomolecules

The carboxyl-functional surface can be evaluated with:

• Antibodies

• Antigens

• Recombinant proteins

• Enzymes

• Peptides

• Lectins

• Amino-modified oligonucleotides

• Other amine-containing ligands

Coupling performance depends on biomolecule purity, concentration, accessible amino groups and reaction conditions.

General EDC/NHS Coupling Workflow

1. Resuspend the Microspheres

Mix CPS/DVB30UM thoroughly before sampling.

2. Wash the Microspheres

Wash the particles with a suitable activation buffer.

3. Activate Surface Carboxyl Groups

Add EDC, with or without NHS, to activate the surface -COOH groups.

4. Add the Biomolecule

Add the antibody, antigen, protein or other amine-containing ligand.

5. Perform Covalent Coupling

Allow the activated carboxyl groups to react with primary amino groups on the biomolecule.

6. Block Residual Reactive Sites

Use a suitable blocking or quenching reagent after coupling.

7. Wash and Resuspend

Remove unbound biomolecules and coupling reagents, then resuspend the functionalized microspheres in the selected storage or assay buffer.

Factors Affecting Coupling Efficiency

Important optimization parameters include:

• Microsphere concentration

• Ligand concentration

• EDC concentration

• NHS concentration

• Activation pH

• Coupling-buffer composition

• Reaction temperature

• Incubation time

• Mixing efficiency

• Blocking conditions

Small-scale optimization is recommended before transferring the process to bulk production.

Immunoassay Development Tips

For more consistent results:

• Use purified antibodies or antigens.

• Avoid unnecessary carrier proteins.

• Keep microspheres uniformly suspended.

• Optimize EDC/NHS concentration.

• Control activation pH carefully.

• Optimize antibody loading.

• Block residual reactive sites.

• Evaluate nonspecific binding.

• Test coupled-particle stability.

• Verify batch-to-batch assay performance.

Crosslinked PS/DVB vs. Standard PS Microspheres

Choose crosslinked PS/DVB when enhanced structural stability and functional surface chemistry are both required.

Covalent Coupling vs. Passive Adsorption

Covalent coupling is preferred when stable biomolecule attachment is important during repeated washing or long-term reagent storage.

Quality Control for Bulk Production

For immunoassay reagent development, recommended evaluation items include:

• Particle-size consistency

• Suspension uniformity

• Solid-content consistency

• Surface carboxyl functionality

• Coupling efficiency

• Ligand loading

• Nonspecific binding

• Signal-to-background ratio

• Assay sensitivity

• Storage stability

• Batch-to-batch reproducibility

Why Choose SHBC CPS/DVB30UM?

• 30 μm carboxyl crosslinked PS/DVB microspheres

• 10% solid-content suspension

• Reactive COOH surface

• Suitable for EDC/NHS coupling

• Designed for antibody and protein immobilization

• Suitable for immunoassay development

• Sample evaluation available

• Stable batch manufacturing

• Bulk production capability

• Custom quantity support

Storage and Handling

Recommended practices include:

• Keep the container tightly sealed.

• Mix thoroughly before sampling.

• Resuspend settled particles before use.

• Avoid unnecessary freezing.

• Prevent microbial contamination.

• Use clean pipettes and containers.

• Avoid conditions that cause irreversible aggregation.

• Validate storage conditions after biomolecule coupling.

Frequently Asked Questions

What is CPS/DVB30UM?

CPS/DVB30UM is a 30 μm carboxyl-functional crosslinked polystyrene/divinylbenzene microsphere product supplied at 10% solids.

What does PS/DVB mean?

PS/DVB refers to polystyrene crosslinked with divinylbenzene.

Why use DVB crosslinking?

DVB creates a more rigid polymer network and can improve heat and solvent resistance compared with non-crosslinked polystyrene.

What is the function of the carboxyl surface?

Surface carboxyl groups provide reactive sites for covalent attachment of antibodies, proteins and other amine-containing biomolecules.

Can antibodies be coupled to CPS/DVB30UM?

Yes. Antibodies can be immobilized using optimized EDC or EDC/NHS coupling chemistry.

Can CPS/DVB30UM be used for immunoassays?

Yes. It can be evaluated for bead-based antibody, antigen and biomarker-detection applications.

What is the solid content?

CPS/DVB30UM is supplied at 10% solids.

Can amino-modified oligonucleotides be coupled?

Yes. Amino-modified DNA or RNA can be evaluated for carbodiimide-mediated coupling to the carboxyl surface.

Why choose 30 μm instead of 20 μm?

30 μm particles provide larger, easier-to-handle solid phases. The preferred size depends on binding capacity, reaction kinetics, washing method and assay format.

Is coupling optimization necessary?

Yes. EDC/NHS concentration, activation pH, ligand loading, reaction time and blocking conditions should be optimized for each project.

Can SHBC supply bulk quantities?

Yes. SHBC supports sample evaluation and bulk supply for immunoassay developers, biotechnology companies and research organizations.

Request CPS/DVB30UM Samples

Contact Shanghai SanYu Biotechnology Co., Ltd. for CPS/DVB30UM samples, technical documentation and bulk quotations.

Please provide:

• Target antibody or protein

• Assay format

• Coupling chemistry

• Required microsphere quantity

• Target application

• Development stage

• Estimated annual demand

CPS/DVB30UM is supplied for research and process development. Final diagnostic applications should be validated by the customer.