Products
SHBC provides colored microspheres, fluorescent microspheres, magnetic beads, silica microspheres, chromatography packing microspheres and biological reagents for diagnostic assay development, nucleic acid extraction, protein purification and separation applications.
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TopGF 6 FF
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SHBC
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6%
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34µm
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200ml 1L 5L 10L 20L
34µm TopGF 6 FF Agarose Gel Filtration Media
SHBC TopGF 6 FF is a highly cross-linked agarose gel filtration medium with an average particle size of 34µm.
The fine and uniform agarose particles provide efficient size-based separation, stable column performance and improved resolution. TopGF 6 FF is suitable for the separation, purification and analysis of polysaccharides, nucleic acids, viruses, proteins and other biological macromolecules.
Product Overview
TopGF 6 FF is an agarose-based size exclusion chromatography resin developed for laboratory purification, process development and industrial downstream applications.
Its highly cross-linked matrix provides good physical and chemical stability. The 34µm particle size creates a uniform packed bed and supports high-resolution biomolecule fractionation.
Key Features and Benefits
Average particle size of 34µm
Highly cross-linked agarose matrix
High-resolution size separation
Good physical and chemical stability
Uniform column packing
Low nonspecific adsorption
Gentle, non-binding separation
Suitable for biological macromolecules
Supports laboratory and process-scale use
Reliable batch-to-batch consistency
Custom packaging and bulk supply available
Product Specifications
Parameter | Specification |
|---|---|
Product Name | TopGF 6 FF |
Brand | SHBC |
Product Type | Agarose gel filtration medium |
Matrix | Highly cross-linked agarose |
Average Particle Size | 34µm |
Separation Mode | Size exclusion chromatography |
Product Form | Agarose resin slurry |
Sterilization Resistance | Up to 121°C under validated conditions |
Main Use | Separation, purification and detection |
Manufacturer | Shanghai SanYu Biotechnology Co., Ltd. |
How TopGF 6 FF Works
TopGF 6 FF separates molecules according to differences in hydrodynamic size.
Large molecules have limited access to the pores of the agarose particles and pass through the column more quickly. Smaller molecules enter more pores and elute later.
Because separation does not depend on strong chemical binding, the method provides gentle conditions for sensitive biological samples.
Recommended Applications
Polysaccharide Purification
Suitable for the fractionation, purification and molecular-size analysis of natural, pharmaceutical and functional polysaccharides.
Nucleic Acid Separation
Can be used for DNA, RNA, plasmids and other nucleic acid purification or analytical fractionation.
Virus Purification
Suitable for virus particle separation, vaccine process development and virus-related purification research.
Protein and Protein Complex Fractionation
Used for separating proteins, aggregates, protein complexes and other biomolecules according to molecular size.
Sample Cleanup
Suitable for removing salts, dyes, small molecules and other low-molecular-weight impurities from macromolecule samples.
Biomolecule Detection
Can be used for sample fractionation before analytical detection, characterization or quality control.
Process Development
Suitable for method screening, purification optimization, pilot production and process scale-up.
Advantages of 34µm Agarose Particles
Improved Separation Resolution
The smaller particle size creates a more uniform packed bed and can improve the separation of molecules with similar sizes.
Efficient Mass Transfer
Shorter diffusion distances support efficient movement of molecules between the mobile phase and internal particle pores.
Stable Packed Bed
The highly cross-linked agarose matrix supports consistent bed structure and reproducible chromatographic performance.
Suitable for Polishing Applications
TopGF 6 FF can be evaluated for intermediate purification and polishing processes where resolution is a key requirement.
Consistent Process Performance
Controlled particle manufacturing supports reliable column packing and batch-to-batch reproducibility.
TopGF 6 FF Application Advantages
Application Requirement | TopGF 6 FF Advantage |
|---|---|
High-resolution fractionation | Fine 34µm particles |
Sensitive biomolecules | Gentle size-based separation |
Stable column operation | Highly cross-linked matrix |
Process consistency | Controlled particle size |
Microbial control | 121°C sterilization capability |
Scale-up development | Laboratory and bulk supply |
General Operating Procedure
Mix the resin slurry gently before use.
Remove the storage solution.
Wash the resin with purified water or equilibration buffer.
Prepare a uniform slurry at the recommended concentration.
Pack the chromatography column at the required bed height.
Equilibrate the column with running buffer.
Clarify and filter the sample before loading.
Apply the sample without disturbing the packed bed.
Elute with the selected buffer.
Collect and analyze the required fractions.
Clean and store the resin according to the validated procedure.
Column dimensions, bed height, sample volume and flow rate should be optimized for the target molecule and required resolution.
Column Packing and Use Recommendations
Fully suspend the resin before packing.
Avoid introducing air bubbles.
Use filtered and degassed buffers.
Clarify samples before loading.
Select a column frit suitable for 34µm particles.
Do not allow the resin bed to dry.
Avoid sudden changes in pressure or flow rate.
Verify column efficiency before purification.
Monitor backpressure during operation.
Cleaning and Sterilization
TopGF 6 FF can withstand high-temperature sterilization at 121°C under validated conditions.
Cleaning and sanitization procedures should be selected according to the sample type, column system and process requirements. Users should validate cleaning agents, contact time, temperature and resin reuse performance in the final application.
Why Choose SHBC TopGF 6 FF
Controlled Agarose Manufacturing
SHBC applies controlled cross-linking and particle preparation processes to support consistent resin quality.
Fine Particle Size
The 34µm agarose particles support efficient packing and high-resolution biomolecule separation.
Stable Matrix Performance
The highly cross-linked structure provides improved physical and chemical stability for repeated chromatography processes.
Flexible Supply Options
Laboratory samples, pilot quantities, bulk packaging and customized supply are available.
Technical Support
SHBC provides support for resin selection, column packing, process optimization and scale-up evaluation.
OEM and ODM Services
Product packaging, storage solution, particle specifications and supply formats can be customized according to project requirements.
Custom and Bulk Supply
Shanghai SanYu Biotechnology Co., Ltd. supplies TopGF 6 FF to universities, research institutes, biotechnology companies, pharmaceutical manufacturers and downstream purification developers.
Available services include:
Laboratory sample supply
Pilot-scale quantities
Bulk resin production
Custom packaging volume
Custom storage solution
Batch quality documentation
Column packing guidance
Purification process support
OEM and ODM manufacturing
Please provide the target molecule, sample volume, column size and estimated annual demand when requesting a quotation.
Frequently Asked Questions
What is TopGF 6 FF?
TopGF 6 FF is a highly cross-linked agarose gel filtration medium used to separate biological molecules according to size.
What is the particle size?
The average particle size is approximately 34µm.
What samples can be purified?
Typical samples include polysaccharides, DNA, RNA, viruses, proteins, protein complexes and other biological macromolecules.
What is the advantage of 34µm particles?
The fine particle size supports a uniform packed bed and improved separation resolution.
Can TopGF 6 FF withstand sterilization?
Yes. The resin can withstand sterilization at 121°C under validated operating conditions.
Does TopGF 6 FF bind the target molecule?
TopGF 6 FF mainly separates molecules according to size rather than specific chemical binding.
Is it suitable for process-scale purification?
Yes. It can be evaluated for laboratory development, pilot production and industrial purification processes.
Can SHBC provide bulk quantities?
Yes. SHBC supports sample evaluation, pilot orders, customized packaging and bulk production.
Storage and Handling
Store TopGF 6 FF according to the conditions stated in the product documentation.
Do not freeze or allow the resin to dry. Keep the container tightly closed and avoid microbial contamination. Mix gently before use and validate storage, cleaning and sterilization procedures for the final process.
Request a Sample or Quotation
Contact Shanghai SanYu Biotechnology Co., Ltd. for samples, technical documentation, customized packaging or bulk pricing.
Product: TopGF 6 FF
Brand: SHBC
Matrix: Highly cross-linked agarose
Average Particle Size: 34µm
Sterilization Resistance: Up to 121°C
Main Applications: Polysaccharide, nucleic acid, virus and biomacromolecule purification
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
For research and industrial purification use.