Products
SHBC provides colored microspheres, fluorescent microspheres, magnetic beads, silica microspheres, chromatography packing microspheres and biological reagents for diagnostic assay development, nucleic acid extraction, protein purification and separation applications.
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TopGF 6 HP
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SHBC
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6%
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34µm
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200ml 1L 5L 10L 20L
34µm TopGF 6 HP High-Performance Agarose Resin
SHBC TopGF 6 HP is a high-performance agarose gel filtration medium with an average particle size of 34µm.
The fine and uniform particles provide high-resolution size exclusion separation for proteins, protein complexes, polysaccharides, nucleic acids, viruses and other biological macromolecules.
TopGF 6 HP is suitable for laboratory purification, process development, intermediate purification and polishing applications.
Product Overview
TopGF 6 HP is a porous agarose-based size exclusion chromatography resin.
Its fine particle size provides a uniform packed bed, efficient mass transfer and improved separation between biomolecules with similar hydrodynamic sizes.
The stable agarose matrix supports reproducible column packing and reliable chromatographic performance.
Key Features and Benefits
Average particle size of 34µm
High-performance agarose matrix
High-resolution size separation
Uniform spherical particles
Stable packed-bed performance
Low nonspecific adsorption
Gentle, non-binding separation
Suitable for sensitive biomolecules
Good batch-to-batch consistency
Suitable for intermediate and polishing steps
Laboratory and bulk supply available
Custom packaging supported
Product Specifications
Parameter | Specification |
|---|---|
Product Name | TopGF 6 HP |
Brand | SHBC |
Product Type | High-performance agarose chromatography resin |
Average Particle Size | 34µm |
Separation Mode | Size exclusion chromatography |
Alternative Name | Agarose gel filtration media |
Product Form | Agarose resin slurry |
Main Use | Separation, purification and analysis |
Manufacturer | Shanghai SanYu Biotechnology Co., Ltd. |
Additional parameters such as fractionation range, operating pressure, pH stability and storage solution are available in the product technical documentation.
How TopGF 6 HP Works
TopGF 6 HP separates biological molecules according to hydrodynamic size.
Large molecules have limited access to the internal pores of the agarose particles and pass through the column more quickly. Smaller molecules enter more pores and therefore elute later.
Because the separation does not require strong chemical binding, TopGF 6 HP provides gentle conditions for sensitive biomolecules.
Recommended Applications
Protein Purification
Suitable for protein fractionation, aggregate removal, monomer separation and protein purity improvement.
Protein Complex Separation
Can be used to separate protein complexes, oligomers and biomolecular assemblies according to size.
Polysaccharide Purification
Suitable for the separation and molecular-size analysis of natural, pharmaceutical and functional polysaccharides.
Nucleic Acid Separation
Can be used for DNA, RNA, plasmids and other nucleic acid purification or analytical fractionation.
Virus and Virus-Like Particle Purification
Suitable for virus particle, virus-like particle and vaccine process research.
Aggregate Removal
The high-resolution matrix can help separate target biomolecules from aggregates and other high-molecular-weight impurities.
Desalting and Sample Cleanup
Can be evaluated for removing salts, dyes, nucleotides and other low-molecular-weight impurities.
Intermediate Purification
Suitable for purification steps requiring improved resolution after initial capture.
Polishing Chromatography
Can be used in final purification stages to improve product purity and remove size-related impurities.
Advantages of 34µm HP Agarose Particles
High Separation Resolution
The fine 34µm particles create a uniform packed bed and support efficient separation of molecules with similar sizes.
Sharp Chromatographic Peaks
Controlled particle size helps reduce band broadening and supports concentrated target fractions.
Efficient Mass Transfer
Short diffusion distances allow molecules to enter and leave the particle pores efficiently.
Stable Column Packing
Uniform agarose particles support consistent bed structure and reproducible purification results.
Suitable for Polishing Steps
TopGF 6 HP is suitable for applications where purity and resolution are more important than maximum processing speed.
TopGF 6 HP vs. TopGF 6 FF
Feature | TopGF 6 HP | TopGF 6 FF |
|---|---|---|
Average Particle Size | 34µm | Larger fast-flow particles |
Main Advantage | High resolution | Higher flow capacity |
Packed-Bed Pressure | Relatively higher | Relatively lower |
Sample Requirement | Well clarified | More process tolerant |
Recommended Use | Intermediate and polishing | Process and scale-up purification |
Separation Efficiency | Higher | Balanced |
Processing Speed | Moderate | Faster |
TopGF 6 HP is recommended when high resolution and purity are the main priorities. TopGF 6 FF is preferred when faster processing and lower backpressure are required.
General Operating Procedure
Mix the resin slurry gently before use.
Remove the storage solution.
Wash the resin with purified water or equilibration buffer.
Prepare a uniform slurry at the recommended concentration.
Pack the chromatography column using controlled flow or pressure.
Equilibrate the packed column with running buffer.
Clarify and filter the sample before loading.
Apply the sample at the selected flow rate.
Elute with the same buffer.
Collect and analyze the required fractions.
Clean and store the resin according to the validated procedure.
Column dimensions, bed height, sample volume and flow rate should be optimized according to the target molecule and required resolution.
Column Packing Recommendations
Fully suspend the resin before packing.
Avoid introducing air bubbles.
Use filtered and degassed buffers.
Select a column frit suitable for 34µm particles.
Clarify samples before loading.
Do not allow the packed bed to dry.
Avoid sudden pressure changes.
Monitor column backpressure.
Evaluate packing efficiency before purification.
Sample Preparation Recommendations
Because TopGF 6 HP uses fine particles, proper sample preparation is important.
Recommended pretreatment includes:
Centrifugation to remove particles
Filtration before sample loading
Removal of visible precipitates
Reduction of excessive sample viscosity
Buffer compatibility testing
Avoidance of sample overloading
Clean samples help maintain column performance and extend resin service life.
Why Choose SHBC TopGF 6 HP
Controlled Particle Manufacturing
SHBC applies controlled agarose particle preparation and classification processes to support consistent 34µm particle size.
High-Resolution Performance
The fine agarose particles are designed for applications requiring accurate size-based separation.
Reliable Batch Consistency
Controlled production and quality inspection support reproducible performance between batches.
Flexible Supply
Laboratory samples, pilot quantities and bulk packaging are available.
Technical Support
SHBC provides support for resin selection, column packing, sample preparation and process optimization.
OEM and ODM Services
Packaging volume, storage solution and supply specifications can be customized according to project requirements.
Custom and Bulk Supply
Shanghai SanYu Biotechnology Co., Ltd. supplies TopGF 6 HP to universities, research institutes, biotechnology companies, pharmaceutical manufacturers and purification process developers.
Available services include:
Laboratory samples
Pilot-scale quantities
Bulk resin production
Custom packaging volume
Custom storage solution
Batch quality documentation
Column packing guidance
Purification process support
OEM and ODM manufacturing
Please provide the target molecule, required purity, column dimensions, sample volume and estimated annual demand when requesting a quotation.
Frequently Asked Questions
What is TopGF 6 HP?
TopGF 6 HP is a high-performance agarose gel filtration medium used to separate biological molecules according to size.
What is the particle size?
The average particle size is approximately 34µm.
What does HP mean?
HP means High Performance. It indicates that the resin is designed for high-resolution chromatographic separation.
What samples can be purified?
Typical samples include proteins, protein complexes, polysaccharides, DNA, RNA, viruses and other biological macromolecules.
What is the main advantage of 34µm particles?
The fine particle size provides a uniform packed bed and improved separation resolution.
What is the difference between TopGF 6 HP and TopGF 6 FF?
TopGF 6 HP focuses on high resolution, while TopGF 6 FF focuses on faster flow and process productivity.
Is TopGF 6 HP suitable for protein aggregate removal?
Yes. It can be evaluated for separating protein monomers from aggregates and other size-related impurities.
Can it be used for polishing chromatography?
Yes. TopGF 6 HP is suitable for intermediate and polishing steps requiring improved purity.
Does the resin bind the target molecule?
TopGF 6 HP mainly separates molecules according to size rather than specific chemical binding.
Can SHBC provide bulk quantities?
Yes. SHBC supports sample evaluation, pilot orders, customized packaging and bulk production.
Storage and Handling
Store TopGF 6 HP according to the conditions stated in the product documentation.
Do not freeze or allow the resin to dry. Keep the container tightly closed and avoid microbial contamination. Mix gently before use and validate cleaning and storage procedures for the final process.
Request a Sample or Quotation
Contact Shanghai SanYu Biotechnology Co., Ltd. for samples, technical documentation, customized packaging or bulk pricing.
Product: TopGF 6 HP
Brand: SHBC
Product Type: High-performance agarose gel filtration media
Average Particle Size: 34µm
Separation Mode: Size exclusion chromatography
Main Applications: Protein, polysaccharide, nucleic acid and virus purification
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
For research and industrial purification use.