34μm Agarose Series Gel Filter Packing GF 30 TopGF 30

34µm TopGF 30 Agarose Gel Filtration Media

SHBC TopGF 30 is a high-resolution agarose-based gel filtration medium with an average particle size of 34µm.

It is developed for size exclusion separation, purification and analysis of peptides, oligosaccharides, small proteins and other low-molecular-weight biomolecules.

The fine and uniform particles provide efficient mass transfer, sharp chromatographic peaks and reliable purification performance.

Product Overview

TopGF 30 is an agarose-based size exclusion chromatography resin designed for fine purification and polishing applications.

Its porous gel structure separates molecules according to differences in hydrodynamic size. The hydrophilic matrix helps reduce nonspecific adsorption and supports high recovery of sensitive biological samples.

TopGF 30 can be used in laboratory research, process development and preparative purification.

Key Features and Benefits

• Average particle size of 34µm

• High-resolution size separation

• Uniform particle size distribution

• Hydrophilic agarose-based matrix

• Low nonspecific adsorption

• High biomolecule recovery

• Sharp and reproducible peaks

• Suitable for peptides and small proteins

• Suitable for fine purification and polishing

• Reliable batch-to-batch consistency

• Laboratory and bulk packaging available

• OEM and ODM supply supported

Product Specifications

Technical parameters such as fractionation range, operating pressure, pH stability and storage solution should be confirmed using the SHBC product technical data sheet.

How TopGF 30 Works

TopGF 30 separates molecules according to their size and ability to enter the pores of the gel particles.

Larger molecules have less access to the internal pores and move through the column more quickly. Smaller molecules enter more pores and therefore require more time to pass through the packed bed.

This non-binding separation mechanism provides gentle purification conditions and helps maintain the structure and activity of sensitive samples.

Recommended Applications

Peptide Purification

Suitable for separating, purifying and polishing synthetic peptides, biological peptides and peptide-based products.

Small Protein Separation

Can be used for the purification and analysis of low-molecular-weight proteins and protein fragments.

Oligosaccharide Fractionation

Suitable for the separation and molecular-size analysis of oligosaccharides and low-molecular-weight polysaccharide fractions.

Biomolecule Polishing

TopGF 30 can be used in final purification steps to remove size-related impurities and improve target product purity.

Aggregate and Fragment Separation

Can help separate small proteins or peptides from aggregates, fragments and other molecular-size variants.

Desalting and Buffer Exchange Research

May be evaluated for separating small biomolecules from salts and other low-molecular-weight components when the selected separation range is suitable.

Product Formulation

Suitable for size-based sample cleanup before formulation, stability evaluation and analytical testing.

Quality Control Analysis

Can be used to evaluate molecular-size distribution, purity and sample homogeneity.

Advantages of 34µm GF30 Media

High Separation Resolution

The fine 34µm particles create a uniform packed bed and improve the separation of molecules with similar sizes.

Efficient Mass Transfer

Short diffusion distances allow molecules to move efficiently between the mobile phase and the internal pores.

Sharp Chromatographic Peaks

Uniform particles help reduce band broadening and produce concentrated target fractions.

Low Nonspecific Adsorption

The hydrophilic matrix reduces unwanted interactions with proteins, peptides and other biomolecules.

High Sample Recovery

Gentle size exclusion separation helps reduce sample loss during purification.

Suitable for Polishing

TopGF 30 is designed for applications where resolution and final product purity are more important than maximum processing speed.

TopGF 30 Application Guide

General Operating Procedure

1. Mix the resin slurry gently before use.

2. Transfer the required volume into a suitable container.

3. Remove the storage solution.

4. Wash the resin with purified water or equilibration buffer.

5. Prepare a uniform resin slurry.

6. Pack the chromatography column under controlled flow or pressure.

7. Equilibrate the packed bed with running buffer.

8. Clarify and filter the sample before loading.

9. Apply the sample at the optimized flow rate.

10. Elute using the selected running buffer.

11. Collect and analyze the required fractions.

12. Clean and store the resin according to the validated procedure.

Sample volume, bed height, flow rate and buffer conditions should be optimized according to the target molecule and required resolution.

Column Packing Recommendations

• Fully suspend the resin before packing.

• Avoid introducing air bubbles into the slurry.

• Use filtered and degassed buffers.

• Select a column frit suitable for 34µm particles.

• Keep the slurry concentration uniform.

• Do not allow the packed bed to dry.

• Avoid sudden changes in flow or pressure.

• Check bed uniformity before sample loading.

• Evaluate column efficiency before purification.

Sample Preparation Recommendations

Proper sample preparation helps maintain resolution and resin service life.

Recommended pretreatment includes:

• Centrifugation to remove particles

• Filtration before sample loading

• Removal of visible precipitates

• Reduction of excessive sample viscosity

• Buffer compatibility evaluation

• Avoidance of excessive sample volume

• Avoidance of column overloading

Why Choose SHBC TopGF 30

Controlled Particle Manufacturing

SHBC applies controlled particle preparation and classification processes to support consistent 34µm particle size.

High-Resolution Purification

TopGF 30 is designed for peptides, oligosaccharides, small proteins and other low-molecular-weight biomolecules.

Reliable Batch Consistency

Controlled manufacturing and quality inspection support reproducible performance between production batches.

Flexible Packaging

Laboratory samples, pilot quantities and bulk packaging are available according to project requirements.

Scale-Up Support

SHBC supports method development, pilot-scale evaluation and preparative purification projects.

Technical Service

Technical support is available for resin selection, column packing, sample loading and process optimization.

Custom and Bulk Supply

Shanghai SanYu Biotechnology Co., Ltd. supplies TopGF 30 to universities, research institutes, biotechnology companies, peptide manufacturers and pharmaceutical process developers.

Available services include:

• Laboratory sample supply

• Pilot-scale quantities

• Bulk resin production

• Custom packaging volume

• Custom storage solution

• Batch quality documentation

• Column packing guidance

• Purification process support

• OEM and ODM manufacturing

Please provide the target molecule, molecular weight, required purity, sample volume and estimated annual demand when requesting a quotation.

Frequently Asked Questions

What is TopGF 30?

TopGF 30 is a 34µm agarose-based gel filtration medium used for high-resolution separation according to molecular size.

What samples are suitable for TopGF 30?

Typical samples include peptides, oligosaccharides, small proteins, protein fragments and other low-molecular-weight biomolecules.

What is the main advantage of 34µm particles?

The fine particle size creates a uniform packed bed and improves chromatographic resolution.

Is TopGF 30 suitable for peptide purification?

Yes. TopGF 30 is suitable for peptide separation, fine purification, polishing and product formulation research.

Can it be used for large antibodies or viruses?

TopGF 30 is mainly designed for relatively small biomolecules. Larger proteins, antibodies or viruses normally require a gel filtration medium with a larger pore size.

Does TopGF 30 bind the target molecule?

No specific ligand binding is required. The primary separation mechanism is based on molecular size.

Can TopGF 30 remove aggregates?

It can be evaluated for separating peptides or small proteins from aggregates and other size-related impurities.

Is TopGF 30 suitable for polishing chromatography?

Yes. Its fine particles and high-resolution performance make it suitable for intermediate and final polishing steps.

Can SHBC provide bulk quantities?

Yes. SHBC supports sample testing, pilot orders, custom packaging and bulk manufacturing.

Storage and Handling

Store TopGF 30 according to the conditions specified in the SHBC technical documentation.

Do not freeze or allow the resin to dry. Keep the container tightly closed and avoid microbial contamination. Mix gently before use and validate cleaning and storage conditions for the final process.

Request a Sample or Quotation

Contact Shanghai SanYu Biotechnology Co., Ltd. for samples, technical documentation, customized packaging or bulk pricing.

Product: TopGF 30
Brand: SHBC
Product Type: Agarose-based gel filtration media
Average Particle Size: 34µm
Separation Mode: Size exclusion chromatography
Main Applications: Peptide, oligosaccharide and small protein purification
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.

For research and industrial purification use.