34umAgarose Series Gel Filter Packing GF 75 TopGF 75

34µm TopGF 75 Agarose Gel Filtration Media

SHBC TopGF 75 is a high-resolution agarose-based gel filtration medium with an average particle size of 34µm.

It is developed for size exclusion separation, purification and analysis of recombinant proteins, enzymes, protein fragments and other medium-sized biomolecules.

The fine and uniform particles provide efficient mass transfer, sharp chromatographic peaks and reliable purification performance.

Product Overview

TopGF 75 is an agarose-based size exclusion chromatography resin for protein purification, process development and polishing applications.

Its porous matrix separates biomolecules according to hydrodynamic size. The hydrophilic surface helps reduce nonspecific adsorption and supports high recovery of sensitive biological materials.

TopGF 75 is suitable for laboratory research, preparative purification and process-scale development.

Key Features and Benefits

• Average particle size of 34µm

• High-resolution size exclusion separation

• Uniform particle size distribution

• Hydrophilic agarose-based matrix

• Low nonspecific adsorption

• High protein recovery

• Sharp and reproducible peaks

• Suitable for recombinant proteins

• Effective separation of aggregates and fragments

• Suitable for intermediate and polishing steps

• Reliable batch-to-batch consistency

• Laboratory and bulk packaging available

Product Specifications

Exact fractionation range, pH stability, operating pressure and storage solution should be confirmed using the SHBC product technical data sheet.

How TopGF 75 Works

TopGF 75 separates biomolecules according to their size and ability to enter the pores of the gel particles.

Larger molecules have limited access to the internal pores and pass through the column more quickly. Smaller molecules enter more pores and elute later.

This non-binding separation mechanism provides gentle purification conditions and helps preserve protein structure and biological activity.

Recommended Applications

Recombinant Protein Purification

Suitable for preparative purification and polishing of recombinant proteins produced in microbial, insect or mammalian expression systems.

Protein Aggregate Removal

Can separate protein monomers from aggregates and other high-molecular-weight impurities.

Protein Fragment Separation

Suitable for removing degradation products, fragments and low-molecular-weight impurities from target proteins.

Enzyme Purification

Can be used for enzyme fractionation, purity improvement and formulation research.

Protein Complex Analysis

Suitable for separating protein complexes, oligomers and different molecular-size forms.

Intermediate Purification

Can be used after affinity or ion exchange chromatography to improve product purity.

Polishing Chromatography

Suitable for final purification steps requiring removal of aggregates, fragments and other size variants.

Product Formulation

Can be used for protein buffer exchange, sample cleanup and formulation process development when the selected separation range is suitable.

Quality Control Analysis

Suitable for evaluating protein purity, aggregation, degradation and molecular-size distribution.

Advantages of 34µm GF75 Media

High Separation Resolution

The fine 34µm particles form a uniform packed bed and improve separation between proteins with similar hydrodynamic sizes.

Sharp Chromatographic Peaks

Controlled particle size helps reduce band broadening and produces concentrated target fractions.

Efficient Mass Transfer

Short diffusion distances allow biomolecules to move efficiently between the mobile phase and the internal pores.

Low Nonspecific Adsorption

The hydrophilic matrix reduces unwanted interactions with proteins and other biological samples.

High Protein Recovery

Gentle size exclusion separation helps reduce irreversible binding and sample loss.

Suitable for Polishing

TopGF 75 is suitable for purification steps where protein purity and resolution are more important than maximum processing speed.

TopGF 75 vs. TopGF 30

TopGF 30 is recommended for peptides, oligosaccharides and small proteins. TopGF 75 is recommended for recombinant proteins, enzymes and related protein purification applications.

TopGF 75 Application Advantages

General Operating Procedure

1. Mix the resin slurry gently before use.

2. Transfer the required resin volume into a clean container.

3. Remove the storage solution.

4. Wash the resin with purified water or equilibration buffer.

5. Prepare a uniform resin slurry.

6. Pack the chromatography column under controlled flow or pressure.

7. Equilibrate the packed bed with running buffer.

8. Clarify and filter the sample before loading.

9. Apply the sample at the optimized flow rate.

10. Elute using the selected running buffer.

11. Collect and analyze the required fractions.

12. Clean and store the resin according to the validated procedure.

Sample volume, bed height, flow rate and buffer conditions should be optimized according to the target protein and required resolution.

Column Packing Recommendations

• Fully suspend the resin before packing.

• Avoid introducing air bubbles.

• Use filtered and degassed buffers.

• Select a column frit suitable for 34µm particles.

• Maintain a uniform slurry concentration.

• Do not allow the packed bed to dry.

• Avoid sudden pressure or flow changes.

• Check bed uniformity before sample loading.

• Evaluate column efficiency before purification.

Sample Preparation Recommendations

Proper sample preparation helps maintain resolution and extend resin service life.

Recommended pretreatment includes:

• Centrifuging samples to remove particles

• Filtering samples before loading

• Removing visible precipitates

• Reducing excessive sample viscosity

• Matching sample and running buffer conditions

• Avoiding excessive sample volume

• Avoiding column overloading

Why Choose SHBC TopGF 75

Controlled Particle Manufacturing

SHBC applies controlled particle preparation and classification processes to support consistent 34µm particle size.

High-Resolution Protein Purification

TopGF 75 is designed for recombinant proteins, enzymes and other protein purification applications.

Reliable Batch Consistency

Controlled manufacturing and quality inspection support reproducible chromatographic performance.

Flexible Supply Options

Laboratory samples, pilot quantities and bulk packaging are available.

Process Scale-Up Support

SHBC supports method development, pilot-scale evaluation and preparative purification projects.

Technical Service

Technical support is available for resin selection, column packing, sample loading and process optimization.

Custom and Bulk Supply

Shanghai SanYu Biotechnology Co., Ltd. supplies TopGF 75 to universities, research institutes, biotechnology companies, pharmaceutical manufacturers and protein purification developers.

Available services include:

• Laboratory sample supply

• Pilot-scale quantities

• Bulk resin production

• Custom packaging volume

• Custom storage solution

• Batch quality documentation

• Column packing guidance

• Protein purification support

• OEM and ODM manufacturing

Please provide the target protein, molecular weight, required purity, sample volume and estimated annual demand when requesting a quotation.

Frequently Asked Questions

What is TopGF 75?

TopGF 75 is a 34µm agarose-based gel filtration medium used for high-resolution separation of proteins according to molecular size.

What samples are suitable for TopGF 75?

Typical samples include recombinant proteins, enzymes, protein complexes, protein fragments and other medium-sized biomolecules.

What is the main advantage of 34µm particles?

The fine particle size creates a uniform packed bed and improves chromatographic resolution.

Is TopGF 75 suitable for recombinant protein purification?

Yes. It can be used for recombinant protein separation, aggregate removal, polishing and formulation research.

Can TopGF 75 remove protein aggregates?

Yes. It can separate protein monomers from aggregates and other high-molecular-weight impurities.

Can it separate protein fragments?

Yes. TopGF 75 can be evaluated for removing degradation products and low-molecular-weight protein fragments.

Does TopGF 75 bind the target protein?

No specific ligand binding is required. The primary separation mechanism is based on molecular size.

Is TopGF 75 suitable for polishing chromatography?

Yes. Its fine particles and high-resolution performance make it suitable for intermediate and final polishing steps.

What is the difference between TopGF 75 and TopGF 30?

TopGF 30 is mainly used for peptides and small proteins, while TopGF 75 is designed for recombinant proteins and medium-sized protein samples.

Can SHBC provide bulk quantities?

Yes. SHBC supports sample testing, pilot orders, custom packaging and bulk manufacturing.

Storage and Handling

Store TopGF 75 according to the conditions specified in the SHBC technical documentation.

Do not freeze or allow the resin to dry. Keep the container tightly closed and avoid microbial contamination. Mix gently before use and validate cleaning and storage conditions for the final process.

Request a Sample or Quotation

Contact Shanghai SanYu Biotechnology Co., Ltd. for samples, technical documentation, customized packaging or bulk pricing.

Product: TopGF 75
Brand: SHBC
Product Type: Agarose-based gel filtration media
Average Particle Size: 34µm
Separation Mode: Size exclusion chromatography
Main Applications: Recombinant protein purification, aggregate removal and polishing
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.

For research and industrial purification use.