3um Carboxyl Blue-Fluo Microspheres CFB3UM-10 1%

3um Carboxyl Blue-Fluo Microspheres CFB3UM-10

SHBC CFB3UM-10 are 3um blue fluorescent microspheres with reactive carboxyl groups on the particle surface.

With excitation at 366nm and emission at 460nm, these microspheres are suitable for flow cytometry, fluorescence microscopy, cell tracking, particle-based assays and biomolecule conjugation.

Product Specifications

Key Product Advantages

• Blue fluorescence at 460nm

• Compatible with suitable 366nm UV excitation

• Reactive carboxyl-functionalized surface

• Uniform nominal 3um particle size

• Easy observation under fluorescence microscopy

• Suitable for antibody and protein conjugation

• Clear particle identification in flow systems

• Research samples and bulk supply available

Why Choose 3um Blue Fluorescent Microspheres?

The 3um particle size provides clear optical recognition and a strong particle-level fluorescent signal.

CFB3UM-10 is suitable for applications requiring:

• Easy particle counting

• Clear flow cytometry discrimination

• Convenient microscopic tracking

• Large biomolecule-coupling surface

• Particle–cell interaction analysis

• Multicolor particle differentiation

Carboxyl Surface for Biomolecule Coupling

CFB3UM-10 contains surface carboxyl groups that can be activated for covalent conjugation with primary amine-containing biomolecules.

Compatible ligands include:

• Antibodies

• Antigens

• Proteins

• Peptides

• Enzymes

• Oligonucleotides

• Other amine-containing molecules

EDC/NHS chemistry may be used to form stable amide bonds between the microspheres and target biomolecules.

Main Applications

Flow Cytometry

The 3um particle size supports clear particle identification in compatible flow cytometry systems.

Potential uses include:

• Fluorescent particle counting

• Particle population analysis

• Surface-binding assays

• Fluorescence channel evaluation

• Flow cytometry method development

• Particle-based multiplex research

The instrument should include a compatible UV excitation source and blue fluorescence detection channel.

Fluorescence Microscopy

CFB3UM-10 can be used for:

• Particle localization

• Fluorescence imaging

• Particle tracking

• Surface-binding studies

• Material distribution analysis

• Microfluidic visualization

Cell Labeling and Tracking

The microspheres can be evaluated for research involving fluorescent particles and biological cells.

Potential applications include:

• Cell–particle binding

• Cell uptake studies

• Phagocytosis research

• Particle adhesion

• Intracellular localization

• Biological transport studies

Particle concentration and incubation conditions should be optimized for each cell model.

Fluorescence Immunoassays

Antibodies, antigens or proteins can be immobilized on the carboxyl surface to prepare fluorescent capture or detection particles.

Potential formats include:

• Particle-based immunoassays

• Sandwich binding assays

• Competitive assays

• Antibody detection

• Antigen detection

• Fluorescent agglutination research

Multiplex Suspension Assays

Blue fluorescent microspheres can be combined with particles of different sizes, fluorescence colors or surface ligands.

Potential applications include:

• Suspension arrays

• Multiplex immunoassays

• Liquid-phase biochips

• Protein detection panels

• Nucleic acid hybridization research

Biosensors and Microfluidics

CFB3UM-10 can serve as a fluorescent signal carrier in:

• Biosensors

• Microarrays

• Biochips

• Microfluidic devices

• Surface-based detection platforms

• Particle transport studies

Particle Tracking and Tracing

The blue fluorescent signal supports particle movement and distribution research in compatible optical systems.

Applications include:

• Fluid-flow visualization

• Filtration studies

• Particle transport research

• Porous-media analysis

• Material distribution testing

Recommended Coupling Workflow

1. Mix the microsphere suspension thoroughly.

2. Wash with a suitable activation buffer.

3. Activate the surface carboxyl groups using EDC and NHS.

4. Add the antibody, protein or target ligand.

5. Incubate under optimized conditions.

6. Block the remaining active sites.

7. Wash the conjugated microspheres.

8. Resuspend in a suitable storage buffer.

9. Evaluate coupling and fluorescence performance.

Coupling pH, reagent concentration, reaction time and blocking conditions should be optimized for each biomolecule.

Handling Recommendations

• Mix gently before sampling.

• Protect the suspension from strong light.

• Avoid prolonged high-power ultrasonication.

• Do not allow the microspheres to dry.

• Avoid freezing and repeated freeze–thaw cycles.

• Use clean, low-binding consumables.

• Confirm buffer compatibility before scale-up.

• Follow the product label for storage requirements.

Application Notes

For flow cytometry, optimize particle concentration, detector settings, optical filters, flow rate and gating strategy.

For fluorescence microscopy, use an excitation source and emission filter compatible with 366nm excitation and 460nm emission.

Because 3um particles are larger than standard fluorescent nanoparticles used in lateral flow assays, membrane migration must be fully validated before LFIA use.

Customization and Bulk Supply

SHBC supports fluorescent microsphere projects from laboratory evaluation to batch manufacturing.

Customization options include:

• Different particle sizes

• Different fluorescence wavelengths

• Fluorescence intensity adjustment

• Surface carboxyl density

• Alternative functional groups

• Solids-content adjustment

• Custom suspension buffers

• Custom packaging

• OEM and ODM manufacturing

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. manufactures functional microspheres and magnetic beads for fluorescence detection, particle analysis, immunoassays and biological research.

SHBC provides:

• Controlled particle-size production

• Functional surface modification

• Reliable batch consistency

• Biomolecule-coupling support

• Research samples

• Flexible packaging

• Pilot-scale supply

• Bulk manufacturing

Frequently Asked Questions

What is CFB3UM-10?

CFB3UM-10 is a 3um carboxyl-functionalized blue fluorescent microsphere suspension with 1% solids content.

What are the excitation and emission wavelengths?

The excitation wavelength is 366nm, and the emission wavelength is 460nm.

Can CFB3UM-10 be coupled with antibodies?

Yes. Its surface carboxyl groups can be activated using EDC/NHS chemistry for covalent antibody or protein conjugation.

Is CFB3UM-10 suitable for flow cytometry?

Yes, provided the instrument has a compatible UV excitation source, blue emission detector and fluidics suitable for 3um particles.

Can it be used for fluorescence microscopy?

Yes. The 3um particle size supports easy visualization, localization, counting and particle tracking.

Can it be used for cell uptake studies?

CFB3UM-10 can be evaluated for cell uptake, phagocytosis and cell–particle interaction research.

Is it suitable for lateral flow assays?

The 3um particle size is larger than conventional LFIA reporter nanoparticles. Membrane migration and assay compatibility must be validated.

Can SHBC provide samples and bulk quantities?

Yes. SHBC supports research samples, pilot-scale evaluation and bulk manufacturing.

Can the product be customized?

Yes. Particle size, fluorescence wavelength, surface group, solids content, suspension buffer and packaging can be customized.

Ordering Information

Product Name: 3um Carboxyl Blue-Fluo Microspheres
Catalog Number: CFB3UM-10
Particle Size: 3um
Solids Content: 1%
Excitation Wavelength: 366nm
Emission Wavelength: 460nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.

Contact SHBC for samples, technical documents, customized specifications and bulk production quotations.

For research and manufacturing use. Performance in the final application should be validated by the customer.