3um Carboxyl Green-Fluo Microspheres CFG3UM-10 1%

3um Carboxyl Green-Fluo Microspheres CFG3UM-10

SHBC CFG3UM-10 Carboxyl Green-Fluo Microspheres are 3um green fluorescent microspheres with reactive carboxyl groups on the particle surface.

With excitation at 470/488nm and emission at 525nm, the microspheres are suitable for flow cytometry, multiplex assays, fluorescence imaging, cell tracking and biomolecule conjugation.

Product Specifications

Key Product Advantages

• Bright green fluorescence at 525nm

• Compatible with common 488nm laser systems

• Reactive carboxyl surface

• Uniform 3um particle size

• High fluorescence per particle

• Easy detection by microscopy and flow cytometry

• Suitable for protein and antibody conjugation

• Available for research and batch production

Why Choose 3um Green Fluorescent Microspheres?

The 3um particle size provides strong fluorescence and clear particle identification.

Compared with smaller fluorescent particles, 3um microspheres offer:

• Higher fluorescence signal per particle

• Larger surface area for ligand immobilization

• Easier optical observation

• Clear discrimination in flow-based systems

• Good suitability for cell interaction studies

• Flexible use in suspension and multiplex assays

Carboxyl Surface for Biomolecule Coupling

CFG3UM-10 contains surface carboxyl groups that can be activated for covalent conjugation with primary amine-containing biomolecules.

Compatible ligands include:

• Antibodies

• Antigens

• Proteins

• Peptides

• Enzymes

• Oligonucleotides

• Other amino-containing molecules

EDC/NHS coupling chemistry can be used to form stable amide bonds between the microspheres and target biomolecules.

Applications

Flow Cytometry

The 3um particle size and 488nm-compatible fluorescence make CFG3UM-10 suitable for flow cytometry research and particle-based fluorescence analysis.

Typical uses include:

• Fluorescence channel evaluation

• Particle population analysis

• Assay development

• Instrument performance studies

• Fluorescent reference particle research

Multiplex Suspension Assays

Different fluorescent intensities, particle sizes or surface ligands can be used to create distinguishable microsphere populations.

CFG3UM-10 can be evaluated for:

• Multiplex immunoassays

• Suspension arrays

• Liquid-phase biochips

• Protein detection

• Antibody detection

• Nucleic acid hybridization research

Fluorescence Immunoassays

The microspheres can carry both fluorescent signals and biological recognition molecules.

They are suitable for sandwich assays, competitive assays and particle-based binding detection.

Fluorescence Microscopy

The strong green signal and micrometer-scale size support easy visualization under compatible fluorescence microscopes.

Applications include:

• Particle localization

• Fluorescence imaging

• Surface binding studies

• Material distribution studies

• Particle transport analysis

Cell Tracking and Phagocytosis Studies

CFG3UM-10 can be evaluated for cell uptake, phagocytosis, intracellular transport and cell–particle interaction research.

Biosensors and Biochips

The carboxyl-functionalized surface supports the immobilization of biological recognition molecules for biosensors, microarrays, biochips and microfluidic platforms.

Instrument Calibration Research

Uniform fluorescent particles can be used for fluorescence detector evaluation, optical alignment and instrument response studies.

CFG3UM-10 is supplied as a research material and is not sold as a certified calibration standard unless otherwise specified.

Recommended Coupling Workflow

1. Mix the microsphere suspension thoroughly.

2. Wash the microspheres with a suitable activation buffer.

3. Activate the surface carboxyl groups using EDC and NHS.

4. Add the antibody, protein or target ligand.

5. Incubate under optimized reaction conditions.

6. Block the remaining active sites.

7. Wash the conjugated microspheres.

8. Resuspend in a suitable storage buffer.

9. Evaluate coupling efficiency and fluorescence performance.

Coupling reagent concentration, pH, reaction time and blocking conditions should be optimized for each biomolecule.

Handling Recommendations

• Mix or vortex gently before use.

• Protect the product from strong light.

• Avoid prolonged high-power ultrasonication.

• Do not allow the microspheres to dry.

• Avoid repeated freezing and thawing.

• Use clean, low-binding laboratory consumables.

• Confirm buffer compatibility before scale-up.

• Follow the product label and technical documents for storage conditions.

Customization and Bulk Supply

SHBC supports fluorescent microsphere projects from laboratory evaluation to batch manufacturing.

Customization options include:

• Different particle sizes

• Different fluorescence wavelengths

• Fluorescence intensity adjustment

• Surface carboxyl density adjustment

• Alternative functional groups

• Solids-content adjustment

• Custom suspension buffers

• Custom packaging

• OEM and ODM manufacturing

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. manufactures functional microspheres and magnetic beads for fluorescence detection, immunoassays, bioseparation and diagnostic reagent development.

SHBC provides:

• Controlled particle-size production

• Stable fluorescence performance

• Functional surface modification

• Reliable batch-to-batch consistency

• Biomolecule coupling support

• Research samples

• Flexible packaging

• Pilot-scale and bulk production

Frequently Asked Questions

What is CFG3UM-10?

CFG3UM-10 is a 3um carboxyl-functionalized green fluorescent microsphere suspension with 1% solids content.

What are the excitation and emission wavelengths?

The recommended excitation wavelengths are 470nm and 488nm. The emission wavelength is 525nm.

Is CFG3UM-10 compatible with a 488nm laser?

Yes. Its fluorescence profile is compatible with suitable optical systems using 488nm excitation and a green emission detection channel.

Can the microspheres be coupled with antibodies?

Yes. The carboxyl groups can be activated using EDC/NHS chemistry for covalent antibody or protein conjugation.

Is CFG3UM-10 suitable for flow cytometry?

Yes. The 3um size and green fluorescent signal make it suitable for flow cytometry research, particle analysis and multiplex assay development.

Can it be used for cell tracking?

CFG3UM-10 can be evaluated for cell uptake, phagocytosis and cell–particle interaction studies.

Is it suitable for lateral flow assays?

Because 3um particles are larger than commonly used fluorescent LFIA nanoparticles, membrane migration must be validated with the selected membrane, pore size and buffer system.

Can SHBC provide samples?

Yes. Samples are available for coupling evaluation, fluorescence testing and application development.

Is bulk production available?

Yes. SHBC supports pilot-scale orders and bulk manufacturing for research institutions, biotechnology companies and reagent manufacturers.

Can the product be customized?

Yes. Particle size, fluorescence wavelength, surface group, solids content, buffer and packaging can be customized.

Ordering Information

Product Name: 3um Carboxyl Green-Fluo Microspheres
Catalog Number: CFG3UM-10
Particle Size: 3um
Solids Content: 1%
Excitation Wavelength: 470/488nm
Emission Wavelength: 525nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.

Contact SHBC for samples, technical documents, customized specifications and bulk production quotations.

For research and manufacturing use. Performance in the final application should be validated by the customer.