Product Overview
CFG3UM-10 Carboxyl Green Fluorescent Microspheres are high-quality fluorescent polystyrene microspheres designed for fluorescence labeling, cell-based assays, diagnostic development and instrument calibration. With a uniform 3 μm particle size, bright green fluorescence and reactive carboxyl-functionalized surfaces, these microspheres deliver reliable and reproducible performance across a wide range of life science applications.
Supplied as a 1% aqueous suspension, CFG3UM-10 provides excellent suspension stability, strong fluorescence intensity and outstanding batch-to-batch consistency. The surface carboxyl groups can be activated using EDC/NHS coupling chemistry, enabling stable covalent attachment of antibodies, proteins, peptides and other amine-containing biomolecules.
Key Features
Uniform 3 μm particle size
Bright green fluorescence
Surface carboxyl functional groups
High fluorescence intensity
Excellent suspension stability
Low particle aggregation
Compatible with EDC/NHS coupling
High biomolecule conjugation efficiency
Excellent batch-to-batch consistency
Suitable for cell and instrument applications
Technical Specifications
Parameter | Specification |
|---|
Product Code | CFG3UM-10 |
Material | Fluorescent Polystyrene Microspheres |
Particle Size | 3 μm |
Surface | Carboxyl (-COOH) |
Solid Content | 1% Suspension |
Excitation (Ex) | 470/488 nm |
Emission (Em) | 525 nm |
Appearance | Green Fluorescent Suspension |
Storage | 2–8°C |
Packaging | 10 mL / 20 mL / 100 mL |
Applications
Cell Labeling
Flow Cytometry
Fluorescence Microscopy
Cell Tracking
Fluorescence Calibration
Instrument Calibration
Immunoassays
Antibody Conjugation
Biosensors
Life Science Research
Product Advantages
CFG3UM-10 combines bright green fluorescence with highly uniform 3 μm particles and reactive surface carboxyl groups, making it ideal for cell labeling, flow cytometry, fluorescence microscopy and calibration applications. The larger particle size offers stronger fluorescence signals, excellent visibility under fluorescence microscopes and efficient antibody immobilization. These microspheres provide outstanding suspension stability, reproducible fluorescence intensity and consistent analytical performance for research and diagnostic assay development.
Storage and Handling
Store at 2–8°C.
Do not freeze.
Protect from direct light.
Keep the container tightly sealed.
Gently invert before use.
Avoid repeated freeze-thaw cycles.
Prevent microbial contamination during handling.
Customization Service
SANYU GROUP provides customized fluorescent microspheres including:
Customized particle sizes
Customized fluorescence intensity
Customized excitation and emission wavelengths
Surface functionalization
Customized solid content
OEM manufacturing
Bulk production
Custom packaging
Frequently Asked Questions
What are Carboxyl Green Fluorescent Microspheres?
Carboxyl Green Fluorescent Microspheres are fluorescent polystyrene microspheres with reactive carboxyl groups for fluorescence labeling, biomolecule conjugation, cell analysis and diagnostic assay development.
What are the excitation and emission wavelengths of CFG3UM-10?
CFG3UM-10 has excitation wavelengths of 470 nm and 488 nm and an emission wavelength of 525 nm, producing bright green fluorescence compatible with common fluorescence microscopes and flow cytometers.
What applications is CFG3UM-10 suitable for?
CFG3UM-10 is widely used for cell labeling, flow cytometry, fluorescence microscopy, cell tracking, fluorescence calibration, immunoassays, biosensors, antibody conjugation and life science research.
Can antibodies be conjugated to CFG3UM-10?
Yes. The surface carboxyl groups can be activated using EDC/NHS chemistry for covalent conjugation of antibodies, proteins, peptides and other amine-containing biomolecules.
How should CFG3UM-10 be stored?
Store at 2–8°C, protect the suspension from light, avoid freezing, and gently invert before use to maintain uniform particle dispersion and optimal fluorescence performance.
