400nm Carboxyl Orange Fluorescent Microspheres
SHBC CFO400-10 are 400 nm carboxyl orange fluorescent microspheres with surface carboxyl (COOH) groups.
With excitation/emission maxima of 465/585 nm and 1% solids, these fluorescent microspheres combine optical detection with a functional COOH surface for labeling, particle tracking, imaging and bead-based assay development.
CFO400-10 Product Specifications
Parameter | Specification |
|---|
Brand | SHBC |
Catalog No. | CFO400-10 |
Product | Carboxyl Orange Fluorescent Microspheres |
Particle Size | 400 nm |
Surface Group | Carboxyl (COOH) |
Fluorescence Color | Orange |
Excitation Maximum | 465 nm |
Emission Maximum | 585 nm |
Solids Content | 1% |
Key Features and Benefits
400 nm particle size for fluorescence-based research
Orange fluorescence for clear optical detection
Ex/Em 465/585 nm for fluorescence imaging and detection
COOH-functionalized surface for biomolecule conjugation
1% solids for convenient experimental preparation
Suitable for labeling, tracking, imaging and bead-based assays
Available under the SHBC brand
Applications of 400nm Orange Fluorescent Microspheres
CFO400-10 can be used in fluorescence-based research and assay development, including:
Fluorescence imaging
Particle tracking and tracing
Biomolecule labeling
Flow cytometry research
Bead-based assays
Immunoassay development
Lateral flow assay development
Biosensor research
Fluorescent probe development
Cell and particle studies
COOH Surface for Biomolecule Conjugation
CFO400-10 microspheres contain carboxyl (COOH) functional groups on the particle surface.
The COOH groups provide reactive sites for covalent conjugation with amine-containing biomolecules through common coupling methods such as EDC/NHS chemistry.
They can be used for conjugation with:
The combination of orange fluorescence and a functional COOH surface makes CFO400-10 suitable for developing fluorescent probes and functional bead-based assays.
Why Choose SHBC Fluorescent Microspheres?
SHBC CFO400-10 combines 400 nm particle size, orange fluorescence, Ex/Em 465/585 nm, COOH surface functionality and 1% solids in one fluorescent microsphere product.
It is designed for researchers who need fluorescent particles that can be detected optically while providing surface groups for further functionalization.
Shanghai SanYu Biotechnology Co., Ltd. supplies SHBC fluorescent microspheres for research, biotechnology and assay development applications.
Frequently Asked Questions
What are CFO400-10 fluorescent microspheres?
CFO400-10 are 400 nm carboxyl orange fluorescent microspheres with COOH functional groups on the particle surface.
What are the excitation and emission wavelengths?
CFO400-10 has an excitation maximum of 465 nm and an emission maximum of 585 nm.
What is the particle size of CFO400-10?
The nominal particle size is 400 nm (0.4 μm).
What is the solids content?
CFO400-10 is supplied at 1% solids.
Are CFO400-10 microspheres carboxyl functionalized?
Yes. The microspheres have carboxyl (COOH) groups on the particle surface for further functionalization.
Can antibodies or proteins be coupled to CFO400-10?
Yes. Surface COOH groups can be activated using suitable coupling chemistry such as EDC/NHS for conjugation with amine-containing antibodies, proteins and other biomolecules.
What are 400nm orange fluorescent microspheres used for?
Typical research applications include fluorescence imaging, particle tracking, biomolecule labeling, flow cytometry, bead-based assays, immunoassay development and biosensor research.
Who supplies CFO400-10?
CFO400-10 is supplied under the SHBC brand by Shanghai SanYu Biotechnology Co., Ltd.
