Product Overview
CFR400-10 Carboxyl Red Fluorescent Microspheres are high-performance fluorescent polystyrene nanoparticles engineered for fluorescence-based detection and biomolecule conjugation. The combination of bright red fluorescence, uniform particle size distribution and reactive surface carboxyl groups provides reliable performance in immunodiagnostics, biosensors and life science research.
Supplied as a 1% aqueous suspension, CFR400-10 exhibits excellent colloidal stability, consistent fluorescence intensity and outstanding batch-to-batch reproducibility. Surface carboxyl groups can be activated through EDC/NHS chemistry, allowing stable covalent conjugation of antibodies, proteins, peptides and other amine-containing biomolecules.
Key Features
Uniform 400 nm particle size
Bright red fluorescence
Surface carboxyl functional groups
High fluorescence intensity
Excellent particle uniformity
Stable aqueous suspension
Compatible with EDC/NHS coupling
High biomolecule conjugation efficiency
Low nonspecific adsorption
Excellent batch-to-batch consistency
Technical Specifications
Parameter | Specification |
|---|
Product Code | CFR400-10 |
Material | Fluorescent Polystyrene Microspheres |
Particle Size | 400 nm |
Surface | Carboxyl (-COOH) |
Solid Content | 1% Suspension |
Excitation (Ex) | 530 nm |
Emission (Em) | 580 nm |
Appearance | Red Fluorescent Suspension |
Storage | 2–8°C |
Packaging | 10 mL / 20 mL / 100 mL |
Applications
Lateral Flow Assays (LFA)
Fluorescence Immunoassays
Chemiluminescence Immunoassays (CLIA)
Antibody Conjugation
Protein Labeling
Cell Labeling
Biosensors
Molecular Diagnostics
Fluorescence Calibration
Life Science Research
Product Advantages
CFR400-10 provides bright red fluorescence together with a highly reactive carboxyl-functionalized surface, enabling efficient biomolecule conjugation and sensitive fluorescence detection. The 400 nm particle size offers increased fluorescence brightness and higher surface loading capacity than smaller nanoparticles, while maintaining excellent dispersion stability. These characteristics make CFR400-10 well suited for lateral flow assays, fluorescence immunoassays, cell labeling, biosensors and in vitro diagnostic reagent development.
Storage and Handling
Store at 2–8°C.
Do not freeze.
Protect from direct light.
Keep the container tightly sealed.
Gently invert before use.
Avoid repeated freeze-thaw cycles.
Prevent microbial contamination during handling.
Customization Service
SANYU GROUP provides customized fluorescent microspheres including:
Customized particle sizes
Customized fluorescence intensity
Customized excitation and emission wavelengths
Surface functionalization
Customized solid content
OEM manufacturing
Bulk production
Custom packaging
Frequently Asked Questions
What are Carboxyl Red Fluorescent Microspheres?
Carboxyl Red Fluorescent Microspheres are fluorescent polystyrene nanoparticles with reactive carboxyl groups for fluorescence detection, biomolecule conjugation and diagnostic assay development.
What are the excitation and emission wavelengths of CFR400-10?
CFR400-10 has an excitation wavelength of 530 nm and an emission wavelength of 580 nm, producing bright red fluorescence for fluorescence imaging and analytical detection.
What applications is CFR400-10 suitable for?
CFR400-10 is suitable for lateral flow assays, fluorescence immunoassays, antibody conjugation, protein labeling, cell labeling, biosensors, molecular diagnostics, fluorescence calibration and life science research.
Can antibodies be conjugated to CFR400-10?
Yes. The surface carboxyl groups can be activated using EDC/NHS chemistry to form stable covalent bonds with antibodies, proteins, peptides and other amine-containing biomolecules.
How should CFR400-10 be stored?
Store at 2–8°C, protect the suspension from light, avoid freezing, and gently mix before use to ensure uniform particle dispersion and stable fluorescence performance.
