Product Overview
BCIP (5-Bromo-4-chloro-3-indolyl Phosphate, Disodium Salt) is a widely used chromogenic substrate for alkaline phosphatase (AP). After enzymatic hydrolysis by alkaline phosphatase, BCIP produces an insoluble blue-colored precipitate, allowing sensitive visualization of target proteins and nucleic acids.
BCIP is commonly used alone or together with NBT (Nitro Blue Tetrazolium) to enhance signal intensity. It is extensively applied in Western blotting, immunohistochemistry (IHC), immunocytochemistry (ICC), ELISA, Southern blotting, Northern blotting and in situ hybridization.
Key Features
High purity biochemical grade
High sensitivity chromogenic substrate
Specific for alkaline phosphatase
Stable color development
Low background staining
Excellent signal intensity
Compatible with BCIP/NBT systems
Reliable batch-to-batch consistency
Easy to prepare and use
Ideal for life science research
Technical Specifications
Parameter | Specification |
|---|
Product Code | RJ003 |
Product Name | BCIP Disodium Salt |
Chemical Name | 5-Bromo-4-chloro-3-indolyl Phosphate Disodium Salt |
CAS No. | 102185-33-1 |
Molecular Formula | C8H6BrClNO4PNa2 |
Molecular Weight | 376.44 g/mol |
Purity | Biochemical Grade |
Appearance | White to Off-white Powder |
Solubility | Soluble in Water |
Target Enzyme | Alkaline Phosphatase (AP) |
Package | 1 g / 5 g / 10 g |
Applications
Western Blotting
Immunohistochemistry (IHC)
Immunocytochemistry (ICC)
ELISA
Southern Blotting
Northern Blotting
In Situ Hybridization
Molecular Biology
Diagnostic Reagent Development
Biotechnology Research
Product Advantages
RJ003 BCIP provides highly sensitive and reliable chromogenic detection for alkaline phosphatase-based assays. When used alone or in combination with NBT, it produces a stable, high-contrast blue-purple precipitate, making it an excellent choice for immunodetection and nucleic acid analysis.
Manufactured under strict quality standards, SANYU GROUP BCIP offers excellent purity, stable performance and dependable quality for research laboratories, biotechnology companies and diagnostic reagent manufacturers worldwide.
Storage and Handling
Store at 2–8°C in a dry environment.
Protect from light and moisture.
Keep the container tightly closed after use.
Avoid prolonged exposure to air and humidity.
For research use only.
Packaging
Standard packaging:
OEM packaging and bulk quantities are available upon request.
Frequently Asked Questions (FAQ)
What is BCIP used for?
BCIP is a chromogenic substrate for alkaline phosphatase and is widely used in Western blotting, immunohistochemistry, ELISA and molecular biology research.
Can BCIP be used together with NBT?
Yes. BCIP is frequently combined with NBT (Nitro Blue Tetrazolium) to produce a stronger blue-purple precipitate and improve detection sensitivity.
Which enzyme is BCIP specific for?
BCIP is specifically designed as a substrate for alkaline phosphatase (AP).
How should BCIP be stored?
Store at 2–8°C, protected from light and moisture. Keep the container tightly sealed after opening.
Do you provide OEM packaging?
Yes. SANYU GROUP provides OEM labeling, customized packaging and bulk supply services for distributors, biotechnology companies, diagnostic reagent manufacturers and research laboratories.
