500um Silica Microspheres Si500UM-10 5%

500um Silica Microspheres for Immunoassay R&D

SHBC Si500UM-10 Silica Microspheres are large, spherical silica particles with a nominal diameter of 500 µm and a solid content of 5%.

They are designed for research projects requiring an easy-to-handle silica solid phase, including immunoassay carrier development, biomolecule immobilization, surface modification, packed-bed testing and filtration-assisted separation.

Si500UM-10 is available for laboratory evaluation, pilot production and bulk manufacturing.

Product Specifications

Key Features and Benefits

Large and Easy-to-Handle Particles

The 500 µm particle size allows easier visual handling, washing, recovery and process observation compared with nano- and small-micron particles.

Suitable for Solid-Phase Carrier Research

Si500UM-10 can be evaluated as a solid carrier for immobilizing antibodies, antigens, proteins, peptides and other biomolecules.

Easy Physical Separation

The large particle size supports sedimentation, filtration, mesh-based separation and packed-bed processing without requiring magnetic separation.

Silanol-Rich Surface

The native silica surface contains silanol groups that can support physical adsorption and silane-based surface modification.

Flexible Surface Functionalization

The silica surface may be further modified to introduce amino, carboxyl, epoxy, aldehyde, thiol or other functional groups.

Bulk Production Support

SHBC provides product samples, pilot-scale supply, customized specifications and stable batch manufacturing for research institutions and reagent developers.

Why Choose 500um Silica Microspheres?

The 500 µm size is suitable for assay and separation systems where convenient particle handling is more important than maintaining a stable nanoscale suspension.

Compared with smaller silica particles, large silica microspheres can provide:

• Easier washing and recovery

• Faster sedimentation

• Convenient filtration

• Better suitability for packed-bed formats

• Easier observation during process development

• Reduced dependence on high-speed centrifugation

• Convenient transfer between reaction vessels

• Potential reuse in selected research processes

Because 500 µm is much larger than particles commonly used in suspension immunoassays, customers should verify compatibility with their instrument, reaction vessel and detection format.

Applications in Immunoassay Development

Si500UM-10 can be evaluated for:

• Solid-phase immunoassay carrier development

• Antibody immobilization studies

• Antigen coating research

• Protein adsorption testing

• Biomolecule coupling studies

• Affinity capture carrier development

• Filtration-assisted washing processes

• Packed-bed immunoreaction systems

• Column-based assay development

• Diagnostic reagent raw material research

• Surface chemistry screening

• Bioconjugation process optimization

• Large-particle biosensor research

• Laboratory separation and purification studies

Final performance depends on the biomolecule, surface modification, buffer composition and assay design.

Biomolecule Adsorption and Surface Modification

The native silica surface may be used for preliminary physical adsorption studies.

Adsorption performance can be affected by:

• Buffer pH

• Ionic strength

• Biomolecule concentration

• Protein isoelectric point

• Incubation time

• Mixing method

• Blocking reagent

• Washing conditions

• Storage buffer

For applications requiring stronger biomolecule attachment, the surface can be modified through silane chemistry before covalent coupling.

Potential functional surfaces include:

• Amino-functional silica

• Carboxyl-functional silica

• Epoxy-functional silica

• Aldehyde-functional silica

• Thiol-functional silica

• Biotin-functional silica

• Streptavidin-coated silica

• Protein A or Protein G coated silica

The appropriate surface chemistry should be selected according to the target biomolecule and assay format.

Suitable Solid-Phase Assay Formats

Si500UM-10 is especially suitable for research formats involving physical recovery or fixed positioning of the microspheres.

Potential formats include:

Packed-Bed Systems

Large silica microspheres can be evaluated as a stationary solid phase in columns, cartridges and flow-through research devices.

Filtration-Assisted Assays

The particles may be retained using an appropriate filter or mesh during washing and buffer exchange.

Batch Adsorption Systems

The microspheres can be mixed with target biomolecules and then recovered by sedimentation or filtration.

Visible Carrier Research

The large particle size allows easier observation and transfer during manual assay development.

Reusable Carrier Studies

Researchers can evaluate the regeneration and reuse of functionalized microspheres in suitable laboratory processes.

Recommended Handling

Mix the product gently before use to obtain a representative suspension.

Recommended precautions include:

• Avoid allowing the silica microspheres to dry.

• Use containers with sufficient opening width.

• Select pipette tips or transfer tools suitable for 500 µm particles.

• Avoid narrow tubing that may cause blockage.

• Use gentle mixing to reduce particle damage.

• Confirm that filters and meshes retain the particles.

• Evaluate sedimentation before automated dispensing.

• Optimize washing conditions for each assay.

• Confirm buffer compatibility before scale-up.

Standard pipette tips designed for small particles may not be suitable for transferring 500 µm microspheres.

Application Development Considerations

Before using Si500UM-10 in an immunoassay, customers should confirm:

• Whether the particles can pass through the dispensing system

• Whether the reaction vessel provides sufficient mixing

• Whether sedimentation affects reaction uniformity

• Whether the detection instrument can accommodate large particles

• Whether filtration or mesh separation is required

• Whether physical adsorption provides sufficient binding stability

• Whether surface functionalization is necessary

• Whether the particle size is suitable for the final test format

Application-specific testing is recommended before pilot or commercial-scale production.

Customization and Bulk Manufacturing

Shanghai SanYu Biotechnology Co., Ltd. provides silica microsphere development and manufacturing services for biotechnology companies, diagnostic reagent developers and research institutions.

Available customization may include:

• Different particle sizes

• Different solid contents

• Custom particle size ranges

• Custom dispersion media

• Amino surface modification

• Carboxyl surface modification

• Epoxy surface modification

• Other functional surfaces

• Custom packaging volumes

• Pilot-scale manufacturing

• Bulk production

• OEM and ODM services

Customization feasibility depends on the required particle size, surface chemistry, concentration, packaging and order volume.

Why Choose SHBC?

• Specialized microsphere manufacturer

• Large 500 µm silica particle option

• Standardized 5% solid content

• Support for immunoassay carrier research

• Native and functionalized surface options

• Laboratory sample availability

• Pilot-scale production support

• Stable bulk manufacturing

• Batch-to-batch consistency

• Custom packaging options

• OEM and ODM services

• Technical support for product development

Quality Documentation

Product and batch documentation can be provided according to order requirements.

Available documents may include:

• Certificate of Analysis

• Safety Data Sheet

• Product specification

• Particle size information

• Batch identification

• Storage recommendations

• Handling guidance

Please contact SHBC to confirm the available testing data for the required production batch.

Frequently Asked Questions

What is Si500UM-10?

Si500UM-10 is a 500 µm silica microsphere product with a solid content of 5%, manufactured by Shanghai SanYu Biotechnology Co., Ltd. under the SHBC brand.

What are 500um silica microspheres used for?

They can be evaluated as large solid-phase carriers for biomolecule immobilization, packed-bed testing, filtration-assisted assays, adsorption research and separation process development.

Are 500um silica microspheres suitable for all immunoassays?

No. The 500 µm size is larger than particles normally used in suspension immunoassays. Customers should confirm compatibility with their reaction vessel, dispensing equipment and detection method.

Can antibodies be adsorbed onto the silica surface?

Physical adsorption can be evaluated. Performance depends on antibody properties, buffer pH, ionic strength, incubation time and blocking conditions.

Can the surface be functionalized?

Yes. The native silica surface can be modified to introduce amino, carboxyl, epoxy and other functional groups.

How can the microspheres be separated?

Depending on the assay design, the particles may be separated by sedimentation, filtration, sieving or retention within a packed column.

Can the product be used in automated equipment?

Automation compatibility depends on the tubing diameter, dispensing nozzle, mixing system and reaction vessel. Equipment testing is required before use.

Does SHBC provide customized particle sizes?

Yes. Custom particle sizes, concentrations, surface groups, dispersion media and packaging formats can be discussed.

Are bulk quantities available?

Yes. SHBC supports sample evaluation, pilot orders and bulk production for research and reagent development projects.

Is Si500UM-10 a finished diagnostic reagent?

No. It is a raw material intended for research and diagnostic reagent development. Customers are responsible for application validation and regulatory evaluation.

Request Samples and Bulk Pricing

Contact SHBC for samples, technical specifications, customized silica microspheres and bulk quotations.

Please provide the following information when requesting a quotation:

• Required quantity

• Target application

• Desired packaging volume

• Required surface chemistry

• Preferred dispersion medium

• Expected annual demand

Product: 500um Silica Microspheres
Catalog Number: Si500UM-10
Particle Size: 500 µm
Solid Content: 5%
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
Intended Use: Research Use Only