50nm Carboxyl Red-Fluo Microspheres CFR050-10 Overview

CFR050-10 50nm Carboxyl Red-Fluo Microspheres are high-performance fluorescent polymer microspheres designed for lateral flow immunoassay (LFIA), fluorescent immunoassay development, antibody conjugation, and diagnostic reagent production.

With a particle size of approximately 50nm, carboxyl-functionalized surface chemistry, red fluorescent signal, and 1% solids concentration, CFR050-10 provides a reliable fluorescent labeling platform for research institutions, biotechnology companies, and diagnostic reagent manufacturers.

The surface carboxyl groups enable covalent coupling with antibodies, antigens, proteins, peptides, and other biomolecules through EDC/NHS activation chemistry, supporting the development of highly specific fluorescent conjugates.

CFR050-10 is suitable for laboratory research, assay optimization, pilot production, and bulk manufacturing of fluorescent lateral flow reagents.

Product Specifications of CFR050-10 Red Fluorescent Microspheres

Key Features of 50nm Carboxyl Fluorescent Microspheres

50nm Nanoscale Particle Size for Immunoassay Development

CFR050-10 features a controlled 50nm particle diameter, providing a balance between nanoscale mobility and fluorescent signal performance.

Compared with larger microspheres, 50nm particles provide:

• Higher particle numbers at the same mass concentration

• Increased available surface area

• Efficient biomolecule interaction

• Good dispersion characteristics

• Flexible optimization for fluorescent LFIA systems

The 50nm size range is suitable for researchers evaluating fluorescent nanoparticles as alternatives to traditional visible labels or larger fluorescent beads.

Stable Red Fluorescence for Quantitative Detection

The fluorescent dye is incorporated into the polymer matrix, providing a stable optical signal for fluorescence-based detection.

Red fluorescent microspheres are commonly selected for:

• Fluorescence lateral flow assays

• Quantitative immunochromatography

• Reader-based diagnostic platforms

• Multiplex detection systems

• Research assay development

Compared with visual color-based detection, fluorescence detection enables digital signal measurement and quantitative analysis when combined with suitable optical readers.

Carboxyl Surface Chemistry for Antibody Coupling

The surface of CFR050-10 contains abundant carboxyl functional groups.

Through EDC or EDC/NHS activation, carboxyl groups can react with primary amino groups on:

• Monoclonal antibodies

• Polyclonal antibodies

• Recombinant proteins

• Antigens

• Peptides

• Other biological molecules

This covalent coupling strategy provides stronger attachment compared with simple physical adsorption methods.

Uniform Particle Size and Batch Consistency

Consistent particle size distribution is important for fluorescent conjugate preparation and lateral flow performance.

CFR050-10 is manufactured with controlled particle size distribution to support:

• Reproducible antibody loading

• Stable conjugate preparation

• Consistent membrane migration

• Reliable batch-to-batch performance

Applications of CFR050-10 in Lateral Flow Immunoassays

Fluorescent Lateral Flow Assay (FLFA)

CFR050-10 can be used as a fluorescent reporter material in lateral flow assay development.

Typical applications include:

• Infectious disease testing research

• Biomarker detection

• Food safety testing

• Veterinary diagnostics research

• Environmental monitoring

• Pharmaceutical quality testing

The fluorescent signal can be measured using fluorescence readers for quantitative or semi-quantitative analysis.

Antibody and Protein Conjugation

The carboxyl-functionalized microspheres provide a convenient platform for biomolecule immobilization.

Common coupling targets include:

• Antibodies

• Antigens

• Binding proteins

• Enzymes

• Peptides

• Nucleic acid probes

Researchers can optimize coupling density according to target assay requirements.

Quantitative Immunochromatography Development

Compared with traditional qualitative lateral flow tests, fluorescent LFIA platforms allow:

• Numerical signal output

• Improved analytical evaluation

• Reduced visual interpretation errors

• Integration with portable readers

CFR050-10 provides a fluorescent particle option for companies developing quantitative rapid diagnostic platforms.

Diagnostic Reagent Manufacturing

CFR050-10 supports the transition from laboratory development to commercial reagent manufacturing.

Suitable stages include:

• Initial screening

• Conjugation optimization

• Prototype strip development

• Pilot production

• Process validation

• Bulk reagent manufacturing

How Do 50nm Carboxyl Fluorescent Microspheres Work in LFIA?

In a typical fluorescent lateral flow assay:

1. CFR050-10 microspheres are conjugated with detection antibodies.

2. The sample is added to the test strip.

3. Target molecules bind with fluorescent antibody conjugates.

4. The complex migrates through the membrane.

5. The target is captured at the test line.

6. The fluorescence intensity is measured by a reader.

7. The signal is converted into qualitative or quantitative results.

The final assay performance depends on:

• Antibody affinity

• Coupling efficiency

• Particle concentration

• Membrane selection

• Buffer formulation

• Reader sensitivity

Antibody Coupling Protocol for CFR050-10

A typical carboxyl microsphere conjugation process includes:

Step 1: Microsphere Activation

Carboxyl groups are activated using EDC or EDC/NHS chemistry.

Activation parameters should be optimized according to:

• Particle concentration

• Antibody type

• Buffer condition

• Reaction time

Step 2: Antibody Conjugation

Activated microspheres are reacted with antibody molecules through amide bond formation.

Optimization factors include:

• Antibody concentration

• Particle-to-protein ratio

• Reaction pH

• Temperature

• Incubation time

Step 3: Blocking and Washing

After conjugation, remaining active sites are blocked and unbound proteins are removed.

Step 4: Conjugate Evaluation

The prepared fluorescent conjugate can be evaluated by:

• Particle size analysis

• Fluorescence intensity

• Protein coupling efficiency

• Stability testing

• LFIA strip performance

Advantages Compared with Traditional Colloidal Gold Labels

CFR050-10 fluorescent microspheres provide several advantages for next-generation immunoassay development:

Quality Control and Batch Production Capability

For diagnostic reagent manufacturers, consistent raw materials are essential.

CFR050-10 quality control may include:

• Particle size distribution

• Solid content testing

• Fluorescence intensity analysis

• Surface carboxyl group evaluation

• Dispersion stability

• Batch consistency testing

SHBC supports research customers and industrial partners with:

• Sample evaluation

• Pilot-scale supply

• Bulk production

• Technical documentation

• Customized specifications

Customization Options for Research and Industrial Production

SHBC provides customization services according to customer assay requirements.

Available options include:

Particle Size Customization

Available sizes can be developed according to:

• Migration requirements

• Detection platform

• Membrane characteristics

Fluorescence Customization

Options include:

• Different fluorescent colors

• Customized optical properties

• Reader compatibility optimization

Surface Functionalization

Available surface groups:

• Carboxyl

• Amino

• Streptavidin

• Other customized chemistry

Solid Content Customization

Different concentrations can be prepared according to:

• Coupling process

• Shipping requirements

• Production workflow

OEM and Bulk Manufacturing

Support includes:

• Custom packaging

• Private labeling

• Long-term supply agreements

• Bulk production

Frequently Asked Questions About CFR050-10

What are 50nm Carboxyl Red-Fluo Microspheres?

50nm Carboxyl Red-Fluo Microspheres are fluorescent polymer particles with carboxyl surface groups designed for antibody conjugation, lateral flow assay development, and immunoassay research.

Can CFR050-10 be used for lateral flow assays?

Yes. CFR050-10 is designed for fluorescent lateral flow assay research and reagent development.

How are antibodies attached to CFR050-10?

Antibodies are commonly coupled through EDC/NHS activation, where surface carboxyl groups react with antibody amino groups to form stable covalent bonds.

Why choose 50nm fluorescent microspheres?

50nm particles provide a balance between nanoscale mobility, surface area, and fluorescence performance, making them suitable for flexible LFIA optimization.

Can CFR050-10 be supplied in bulk quantities?

Yes. SHBC provides research samples, pilot batches, and bulk production supply for qualified customers.

Is customization available?

Yes. Particle size, fluorescence properties, surface chemistry, concentration, and packaging can be customized according to project requirements.

Why Choose SHBC CFR050-10 Carboxyl Red-Fluo Microspheres?

SHBC provides high-quality fluorescent microsphere materials for immunodiagnostic research and reagent manufacturing.

CFR050-10 offers:

• 50nm controlled particle size

• Red fluorescent signal

• Carboxyl functional surface

• Reliable antibody coupling capability

• LFIA development support

• Research-to-production supply capability

• OEM and customization services

Contact SHBC for technical specifications, samples, and bulk quotation for CFR050-10 50nm Carboxyl Red-Fluo Microspheres.