5um Carboxyl Crosslinked PS/DVB Microspheres CPS/DVB5UM ,10%

5 μm Carboxyl Crosslinked PS/DVB Microspheres CPS/DVB5UM

SHBC CPS/DVB5UM Carboxyl Crosslinked PS/DVB Microspheres are 5 μm functional polymer microspheres with surface carboxyl groups and a crosslinked polystyrene/divinylbenzene matrix.

Supplied at 10% solids, these microspheres are designed for covalent biomolecule immobilization, immunoassay development, protein coupling and scalable reagent production.

The PS/DVB crosslinked structure provides improved particle rigidity and chemical stability compared with conventional non-crosslinked polystyrene particles.

CPS/DVB5UM Technical Specifications

Crosslinked PS/DVB Microsphere Structure

CPS/DVB5UM microspheres use divinylbenzene to crosslink the polystyrene matrix.

Crosslinking helps provide:

• Improved particle rigidity

• Better structural stability

• Improved resistance to heat

• Improved resistance to compatible organic environments

• Stable particle morphology during processing

• Improved suitability for repeated washing procedures

DVB-crosslinked polystyrene particles are commonly selected when greater structural stability is required than standard non-crosslinked PS microspheres.

Carboxyl Surface for Covalent Coupling

The microsphere surface contains reactive carboxyl groups that can be activated for covalent attachment of biomolecules.

Typical coupling chemistry uses:

Carboxyl Group → EDC or EDC/NHS Activation → Amine-Containing Biomolecule → Stable Amide Bond

Compatible biomolecules may include:

• Antibodies

• Antigens

• Recombinant proteins

• Peptides

• Enzymes

• Amino-modified oligonucleotides

• Other amine-containing ligands

Carboxyl-functional microspheres are widely used for covalent protein and ligand immobilization through carbodiimide-mediated coupling.

Key Advantages of CPS/DVB5UM

• 5 μm particle size

• 10% solid content

• Crosslinked PS/DVB matrix

• Reactive carboxyl surface

• Suitable for EDC/NHS coupling

• Stable covalent biomolecule attachment

• Good particle rigidity

• Suitable for repeated washing

• Flexible antibody and antigen functionalization

• Suitable for research and bulk production

Why Choose 5 μm Carboxyl Microspheres?

The 5 μm particle format provides a practical balance between available surface area, particle handling and assay detection.

Potential advantages include:

• Easy centrifugation and washing

• Convenient optical or flow-based detection

• Suitable surface area for biomolecule immobilization

• Straightforward assay optimization

• Suitable for bead-based immunoassays

• Convenient laboratory handling

Spherotech commercially offers crosslinked carboxyl polystyrene particles in the approximately 5 μm size range specifically as functionalized particles for covalent protein or ligand coupling.

Immunoassay Applications

Antibody-Coupled Microspheres

Capture antibodies can be covalently immobilized on CPS/DVB5UM for:

• Bead-based immunoassays

• Antigen detection

• Biomarker detection

• Multiplex assay research

• Serological assay development

• Diagnostic reagent development

Covalent coupling helps reduce ligand desorption compared with simple physical adsorption, which can be advantageous when stable bead reagents are required.

Antigen-Coupled Microspheres

Antigens can be immobilized for:

• Antibody detection

• Serology research

• Autoantibody assay development

• Infectious-disease research

• Assay calibration

• Method development

Flow Cytometry Research

The 5 μm particle size can also be evaluated in bead-based flow cytometry applications such as:

• Antibody-binding studies

• Antigen detection

• Multiplex assay development

• Instrument method development

• Biomolecule-binding analysis

Protein and Biomolecule Applications

CPS/DVB5UM can also be evaluated for:

• Protein immobilization

• Enzyme immobilization

• Peptide coupling

• Affinity ligand immobilization

• Oligonucleotide attachment

• Biosensor development

• Biomolecule capture

• Surface chemistry research

Suitable Coupling Molecules

The carboxyl surface can be evaluated with:

• Monoclonal antibodies

• Polyclonal antibodies

• Recombinant proteins

• Diagnostic antigens

• Peptides

• Enzymes

• Amino-modified DNA

• Amino-modified RNA

• Other amine-containing ligands

Final coupling efficiency depends on ligand purity, concentration and reaction conditions.

General EDC/NHS Coupling Workflow

1. Resuspend the Microspheres

Mix the microsphere suspension thoroughly before sampling.

2. Wash the Microspheres

Wash the particles using a suitable activation or coupling buffer.

3. Activate the Carboxyl Groups

Add EDC, with or without NHS, to activate surface carboxyl groups.

4. Add the Biomolecule

Add the antibody, antigen, protein or other amine-containing ligand.

5. Perform Covalent Coupling

Allow the activated carboxyl groups to react with primary amines on the biomolecule to form stable amide bonds.

6. Block Residual Reactive Sites

Use a suitable blocking or quenching solution after coupling.

7. Wash and Resuspend

Remove unbound biomolecules and coupling reagents, then resuspend the functionalized microspheres in the selected storage or assay buffer.

EDC-mediated coupling conditions should be optimized for each biomolecule because coupling efficiency and retained biological activity depend on buffer, pH, reagent concentration and reaction time.

Factors Affecting Coupling Performance

Important optimization factors include:

• Microsphere concentration

• Antibody or protein concentration

• EDC concentration

• NHS concentration

• Activation pH

• Coupling-buffer composition

• Reaction temperature

• Incubation time

• Mixing efficiency

• Blocking conditions

Small-scale optimization is recommended before production scale-up.

CPS/DVB5UM vs. Standard Polystyrene Microspheres

Choose CPS/DVB5UM when both a functional carboxyl surface and a more structurally stable polymer matrix are required.

Covalent Coupling vs. Passive Adsorption

Covalent immobilization is commonly selected when long-term ligand retention and stable functional microspheres are important.

Quality Control for Immunoassay Production

For research-to-production scale-up, evaluate:

• Particle-size consistency

• Suspension uniformity

• Solid-content consistency

• Carboxyl functionality

• Coupling efficiency

• Antibody loading

• Nonspecific binding

• Signal-to-background ratio

• Assay sensitivity

• Storage stability

• Batch-to-batch reproducibility

Why Choose SHBC CPS/DVB5UM?

• 5 μm carboxyl crosslinked PS/DVB microspheres

• High 10% solid content

• Functional -COOH surface

• Suitable for EDC/NHS coupling

• Designed for antibody and protein immobilization

• Suitable for immunoassay research

• Sample evaluation available

• Stable batch supply

• Bulk manufacturing capability

• Support for research and production quantities

Storage and Handling

Store CPS/DVB5UM according to the product label and technical documentation.

Recommended practices:

• Keep the container tightly sealed.

• Mix thoroughly before sampling.

• Prevent microbial contamination.

• Avoid unnecessary freezing.

• Use clean pipettes and containers.

• Avoid conditions that cause irreversible aggregation.

• Validate storage conditions after biomolecule coupling.

Frequently Asked Questions

What is CPS/DVB5UM?

CPS/DVB5UM is a 5 μm carboxyl-functional crosslinked polystyrene/divinylbenzene microsphere product supplied at 10% solids.

Why is DVB used in the microspheres?

Divinylbenzene crosslinks the polystyrene matrix and helps improve particle rigidity and structural stability.

What does the carboxyl surface do?

Surface carboxyl groups allow antibodies, proteins and other amine-containing biomolecules to be covalently attached.

Can antibodies be coupled to CPS/DVB5UM?

Yes. Antibodies can be immobilized using optimized EDC or EDC/NHS coupling chemistry.

Can the microspheres be used for immunoassays?

Yes. They can be evaluated for bead-based antibody, antigen and biomarker-detection assay development.

What is the solid content?

CPS/DVB5UM is supplied at 10% solids.

Why choose covalent coupling instead of adsorption?

Covalent coupling provides stronger ligand attachment and can reduce biomolecule desorption during washing or storage.

Can amino-modified oligonucleotides be attached?

Yes. Amino-functionalized oligonucleotides can be evaluated for carbodiimide-mediated coupling to carboxyl microspheres.

Is coupling optimization necessary?

Yes. Activation conditions, ligand concentration, pH, reaction time and blocking conditions should be optimized for each biomolecule.

Can SHBC supply bulk quantities?

Yes. SHBC supports sample evaluation and bulk supply for immunoassay developers, biotechnology companies and research organizations.

Request CPS/DVB5UM Samples

Contact Shanghai SanYu Biotechnology Co., Ltd. for CPS/DVB5UM samples, technical documentation and bulk quotations.

Please provide:

• Target antibody or protein

• Assay format

• Coupling chemistry

• Required microsphere quantity

• Target application

• Development stage

• Estimated annual demand

CPS/DVB5UM is supplied for research and process development. Final diagnostic applications should be validated by the customer.