Agarose Series Gel Filter Packing 4 FF 90um TopGF 4 FF

TopGF 4 FF 90µm Fast Flow Agarose Chromatography Resin

SHBC TopGF 4 FF is a highly cross-linked agarose chromatography resin with an average particle size of 90µm.

Developed from the TopGF 4B agarose matrix, TopGF 4 FF has a higher degree of cross-linking than TopGF CL-4B. It provides improved physical and chemical stability, fast flow performance and resistance to sterilization at 121°C.

TopGF 4 FF is designed for large-scale separation and purification of polysaccharides, nucleic acids, viruses and other biological macromolecules. It can also be used as a base matrix for affinity, hydrophobic interaction and ion exchange chromatography resins.

Product Overview

TopGF 4 FF is a fast-flow agarose-based size exclusion chromatography medium for laboratory research, process development and industrial purification.

Its highly cross-linked structure supports stable column beds and higher operating flow rates. The porous agarose matrix enables gentle separation according to molecular size without requiring specific binding between the sample and the resin.

Key Features and Benefits

• Average particle size of 90µm

• Highly cross-linked agarose matrix

• Fast flow performance

• Improved physical stability

• Good chemical resistance

• Withstands sterilization at 121°C

• Suitable for large-scale purification

• Gentle size-based separation

• Suitable for biological macromolecules

• Can be used as a functional resin base matrix

• Laboratory and bulk packaging available

• Consistent batch-to-batch performance

Product Specifications

How TopGF 4 FF Works

TopGF 4 FF separates molecules according to differences in hydrodynamic size.

Large molecules have limited access to the pores of the agarose beads and move through the column more quickly. Smaller molecules enter more pores, travel through a longer path and elute later.

This non-binding separation mechanism helps maintain the activity and structure of sensitive biological samples.

Recommended Applications

Polysaccharide Purification

Suitable for the fractionation, purification and molecular-size analysis of natural, pharmaceutical and functional polysaccharides.

Nucleic Acid Separation

Can be used for DNA, RNA and other nucleic acid purification, sample cleanup and analytical fractionation.

Virus Purification

Suitable for virus particle separation, vaccine process development, virus detection and downstream purification research.

Biological Macromolecule Fractionation

Used for separating proteins, protein complexes and other biomolecules according to molecular size.

Sample Cleanup

Can remove small-molecule impurities, salts, dyes and other contaminants from macromolecule samples.

Large-Scale Process Purification

The fast-flow and stable matrix is suitable for pilot-scale and industrial chromatography processes requiring higher productivity.

Chromatography Base Matrix Applications

TopGF 4 FF can be further modified with different ligands or functional groups to prepare specialized chromatography resins.

Affinity Chromatography Base Matrix

The agarose surface can be activated and coupled with antibodies, proteins, enzymes, peptides or other affinity ligands.

Potential products include:

• Protein A affinity resin

• Protein G affinity resin

• Antibody purification resin

• Enzyme immobilization resin

• Custom ligand affinity resin

Hydrophobic Interaction Chromatography Base Matrix

Hydrophobic ligands can be introduced onto the matrix for protein capture and intermediate purification.

Potential functional groups include:

• Butyl

• Octyl

• Phenyl

• Other hydrophobic ligands

Ion Exchange Chromatography Base Matrix

Charged groups can be coupled to the agarose matrix to prepare anion or cation exchange chromatography resins.

Potential functional groups include:

• Q strong anion exchange

• DEAE weak anion exchange

• SP strong cation exchange

• CM weak cation exchange

Advantages of TopGF 4 FF

Higher Cross-Linking

The increased degree of cross-linking provides better matrix strength and stability than conventional TopGF 4B and TopGF CL-4B media.

Fast Flow Performance

The 90µm particles and stable agarose structure support higher flow rates and shorter processing times.

High-Temperature Sterilization

TopGF 4 FF can withstand sterilization at 121°C under validated operating conditions.

Suitable for Scale-Up

The resin is suitable for laboratory development, pilot production and large-scale industrial purification.

Gentle Biomolecule Separation

Size exclusion chromatography does not require strong binding or harsh elution conditions, helping protect sensitive biomolecules.

Flexible Functionalization

The agarose matrix can be modified for affinity, hydrophobic interaction and ion exchange chromatography applications.

TopGF 4 FF Compared with Other TopGF Media

TopGF 4 FF is recommended when fast processing, higher matrix stability and large-scale purification are required.

General Operating Procedure

1. Mix the resin slurry gently before use.

2. Remove the storage solution.

3. Wash the resin with purified water or equilibration buffer.

4. Prepare a uniform slurry at the recommended concentration.

5. Pack the chromatography column at the required bed height.

6. Equilibrate the packed column with running buffer.

7. Clarify or filter the sample before loading.

8. Apply the sample without disturbing the resin bed.

9. Elute with the selected buffer.

10. Collect and analyze the required fractions.

11. Clean and store the resin according to the validated process.

Column dimensions, sample volume, flow rate and buffer conditions should be optimized for the target molecule and required resolution.

Column Packing Recommendations

• Keep the resin uniformly suspended during packing.

• Avoid introducing air bubbles into the slurry.

• Use filtered and degassed buffers when possible.

• Do not allow the resin bed to dry.

• Avoid sudden changes in flow rate or pressure.

• Confirm column efficiency before sample purification.

• Validate packing conditions before process scale-up.

Why Choose SHBC TopGF 4 FF

Controlled Agarose Manufacturing

SHBC applies controlled cross-linking, particle preparation and quality inspection processes to support reliable resin performance.

Stable 90µm Particle Size

Controlled particle size supports consistent column packing, flow performance and process reproducibility.

Large-Scale Supply

SHBC supports laboratory samples, pilot-scale evaluation and bulk resin manufacturing.

Functionalization Support

Custom activation and ligand modification services are available for affinity, hydrophobic interaction and ion exchange resin development.

Flexible Packaging

Packaging volume, storage solution and supply specifications can be customized according to project requirements.

Technical Service

Technical support is available for resin selection, column packing, cleaning, process optimization and scale-up evaluation.

Custom and Bulk Supply

Shanghai SanYu Biotechnology Co., Ltd. supplies TopGF 4 FF to universities, research institutes, biotechnology companies, pharmaceutical manufacturers and chromatography resin developers.

Available services include:

• Laboratory sample supply

• Pilot-scale quantities

• Bulk production

• Custom packaging volume

• Custom storage solution

• Agarose matrix activation

• Ligand coupling services

• OEM and ODM manufacturing

• Batch quality documentation

• Column packing guidance

• Process scale-up support

Please provide the target molecule, purification process, column dimensions and estimated annual demand when requesting a quotation.

Frequently Asked Questions

What is TopGF 4 FF?

TopGF 4 FF is a highly cross-linked fast-flow agarose chromatography resin with an average particle size of 90µm.

What is the difference between TopGF 4 FF and TopGF CL-4B?

TopGF 4 FF has a higher degree of cross-linking and is designed to provide improved stability, faster flow and better suitability for large-scale purification.

What samples can be purified?

Typical samples include polysaccharides, DNA, RNA, viruses, proteins, protein complexes and other biological macromolecules.

Can TopGF 4 FF withstand autoclaving?

Yes. The resin can withstand sterilization at 121°C under validated conditions.

Is TopGF 4 FF suitable for industrial purification?

Yes. Its fast-flow performance and stable cross-linked matrix make it suitable for pilot-scale and large-scale chromatography processes.

Can it be used as an affinity chromatography matrix?

Yes. TopGF 4 FF can be activated and coupled with affinity ligands such as antibodies, proteins, enzymes or other biomolecules.

Can it be modified for ion exchange chromatography?

Yes. Anion or cation exchange functional groups can be introduced onto the agarose matrix.

Is hydrophobic modification available?

Yes. Hydrophobic ligands such as butyl, octyl or phenyl groups can be introduced according to project requirements.

Can SHBC provide bulk quantities?

Yes. SHBC supports sample evaluation, pilot orders, customized packaging and bulk manufacturing.

Storage and Handling

Store TopGF 4 FF according to the conditions stated in the product documentation.

Do not freeze or allow the resin to dry. Keep the container tightly closed and avoid microbial contamination. Mix gently before use and validate cleaning, sterilization and storage procedures for the final application.

Request a Sample or Quotation

Contact Shanghai SanYu Biotechnology Co., Ltd. for samples, technical documentation, custom functionalization or bulk pricing.

Product: TopGF 4 FF
Brand: SHBC
Matrix: Highly cross-linked agarose
Average Particle Size: 90µm
Sterilization Resistance: Up to 121°C
Main Application: Fast-flow size exclusion chromatography
Additional Use: Base matrix for affinity, hydrophobic interaction and ion exchange chromatography
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.

For research and industrial purification use.