RJ001 AMPPD (3-(2-Spiroadamantane)-4-Methoxy-4-(3-Phosphoryloxy)Phenyl-1,2-Dioxetane) | CAS 122341-56-4

Product Overview

AMPPD (3-(2'-Spiroadamantane)-4-methoxy-4-(3''-phosphoryloxy)phenyl-1,2-dioxetane Disodium Salt) is one of the most widely used chemiluminescent substrates for alkaline phosphatase (AP) detection. Upon enzymatic dephosphorylation by alkaline phosphatase, AMPPD generates a sustained chemiluminescent signal with exceptionally high sensitivity and very low background.

AMPPD is extensively used in ELISA, Western blotting, Southern blotting, Northern blotting, DNA/RNA hybridization, immunoassays and clinical diagnostic reagent development. Its high signal intensity and long-lasting luminescence make it an excellent choice for sensitive analytical applications.

Key Features

• High purity biochemical grade

• High chemiluminescent sensitivity

• Low background signal

• Excellent signal stability

• Compatible with alkaline phosphatase

• Suitable for immunoassays

• High detection accuracy

• Reliable batch-to-batch consistency

• Easy to prepare and use

• Ideal for life science research

Technical Specifications

Applications

• ELISA

• Western Blotting

• Southern Blotting

• Northern Blotting

• DNA Hybridization

• RNA Hybridization

• Immunoassays

• Molecular Diagnostics

• Clinical Diagnostic Reagent Development

• Biotechnology Research

Product Advantages

RJ001 AMPPD provides exceptionally high chemiluminescent sensitivity with prolonged signal duration, making it one of the preferred substrates for alkaline phosphatase-based detection systems. Its low background emission improves signal-to-noise ratio and enhances analytical accuracy.

Manufactured under strict quality standards, SANYU GROUP AMPPD offers excellent purity, consistent performance and reliable results for research laboratories, biotechnology companies, diagnostic reagent manufacturers and pharmaceutical developers.

Storage and Handling

• Store at 2–8°C in a dry environment.

• Protect from light and moisture.

• Keep the container tightly closed after use.

• Avoid repeated freeze-thaw cycles after solution preparation.

• For research use only.

Packaging

Standard packaging:

• 1 g

• 5 g

• 10 g

OEM packaging and bulk quantities are available upon request.

Frequently Asked Questions (FAQ)

What is AMPPD used for?

AMPPD is primarily used as a chemiluminescent substrate for alkaline phosphatase in ELISA, Western blotting, nucleic acid hybridization and immunoassays.

Which enzyme is compatible with AMPPD?

AMPPD is specifically designed for detection systems using alkaline phosphatase (AP).

Why is AMPPD widely used in immunoassays?

AMPPD produces a strong and stable chemiluminescent signal with very low background, enabling highly sensitive detection of target molecules.

How should AMPPD be stored?

Store at 2–8°C, protected from light and moisture. Keep the container tightly sealed after opening.

Do you provide OEM packaging?

Yes. SANYU GROUP provides OEM labeling, customized packaging and bulk supply services for distributors, diagnostic reagent manufacturers and biotechnology companies.