Carboxyl Blue-Fluo Microspheres CFB200-10 1%

200nm Carboxyl Blue-Fluo Microspheres CFB200-10

SHBC CFB200-10 are 200nm blue fluorescent microspheres with carboxyl-functionalized surfaces.

With excitation at 366nm and emission at 460nm, these microspheres provide a clear blue fluorescent signal for immunoassays, lateral flow test development, biosensors, fluorescence imaging and biomolecule conjugation.

Product Specifications

Key Product Advantages

• Blue fluorescence at 460nm

• Compatible with 366nm UV excitation

• Reactive carboxyl-functionalized surface

• Uniform 200nm particle size

• High surface area for biomolecule loading

• Good dispersion in aqueous systems

• Suitable for qualitative and quantitative detection

• Available for research and batch production

Why Choose 200nm Blue Fluorescent Microspheres?

The 200nm particle size offers a practical balance between fluorescent signal, surface area and suspension stability.

CFG200-10 is suitable for applications requiring:

• Nanoparticle-scale fluorescent labels

• High biomolecule loading capacity

• Stable particle dispersion

• UV-excited blue fluorescence

• Consistent assay migration

• Sensitive optical detection

Carboxyl Surface for Biomolecule Coupling

Surface carboxyl groups can be activated for covalent attachment of primary amine-containing biomolecules.

Compatible ligands include:

• Antibodies

• Antigens

• Proteins

• Peptides

• Enzymes

• Oligonucleotides

• Other amine-containing molecules

EDC/NHS coupling chemistry can be used to form stable amide bonds between the microspheres and target biomolecules.

Main Applications

Fluorescent Lateral Flow Immunoassays

CFB200-10 can be evaluated as a fluorescent reporter particle for lateral flow assay development.

The 200nm particle size supports membrane migration, while the 460nm blue emission enables optical signal detection using a compatible reader.

Fluorescence Immunoassays

The microspheres can carry both fluorescent signals and biological recognition molecules.

Suitable assay formats include:

• Sandwich immunoassays

• Competitive immunoassays

• Antibody detection

• Antigen detection

• Particle-based binding assays

Antibody and Protein Conjugation

The carboxyl surface supports covalent antibody, protein and peptide immobilization for fluorescent probe preparation.

Biosensors and Biochips

CFB200-10 can be used as a fluorescent signal carrier in:

• Biosensors

• Microarrays

• Biochips

• Microfluidic systems

• Surface-based detection platforms

Fluorescence Imaging and Particle Tracing

The blue fluorescent signal supports particle localization, transport studies, material distribution analysis and fluorescence microscopy research.

Multiplex Detection Research

Blue fluorescent microspheres can be combined with green, red or other fluorescent particles to develop distinguishable particle populations for multicolor and multiplex assay research.

Recommended Coupling Workflow

1. Disperse the microsphere suspension thoroughly.

2. Wash with a suitable activation buffer.

3. Activate the carboxyl groups using EDC and NHS.

4. Add the antibody, protein or target ligand.

5. Incubate under optimized conditions.

6. Block the remaining active sites.

7. Wash the conjugated microspheres.

8. Resuspend in a suitable storage buffer.

9. Evaluate coupling and fluorescence performance.

Reaction pH, reagent concentration, coupling time and blocking conditions should be optimized for each biomolecule.

Handling Recommendations

• Mix thoroughly before use.

• Protect the suspension from strong light.

• Avoid prolonged high-power ultrasonication.

• Do not allow the microspheres to dry.

• Avoid freezing and repeated freeze–thaw cycles.

• Use clean, low-binding consumables.

• Confirm buffer compatibility before scale-up.

• Follow the product label for storage conditions.

Customization and Bulk Supply

SHBC supports fluorescent microsphere projects from laboratory evaluation to batch manufacturing.

Customization options include:

• Different particle sizes

• Different fluorescence wavelengths

• Fluorescence intensity adjustment

• Surface carboxyl density adjustment

• Alternative functional groups

• Solids-content adjustment

• Suspension buffer customization

• Custom packaging

• OEM and ODM manufacturing

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. manufactures functional microspheres and magnetic beads for fluorescence detection, immunoassays, diagnostic reagent development and biological research.

SHBC provides:

• Controlled particle-size production

• Stable fluorescence performance

• Functional surface modification

• Reliable batch consistency

• Biomolecule coupling support

• Research samples

• Flexible packaging

• Pilot-scale and bulk production

Frequently Asked Questions

What is CFB200-10?

CFB200-10 is a 200nm carboxyl-functionalized blue fluorescent microsphere suspension with 1% solids content.

What are the excitation and emission wavelengths?

The excitation wavelength is 366nm, and the emission wavelength is 460nm.

Can CFB200-10 be coupled with antibodies?

Yes. The surface carboxyl groups can be activated using EDC/NHS chemistry for covalent antibody or protein conjugation.

Is CFB200-10 suitable for lateral flow assays?

Yes. The 200nm particles can be evaluated as fluorescent reporter labels in lateral flow immunoassays. Membrane, buffer and reader compatibility should be optimized.

What equipment can detect the microspheres?

A fluorescence reader, microscope or optical system equipped with compatible UV excitation and blue emission filters can be used.

Can CFB200-10 be used for multiplex assays?

Yes. It can be combined with microspheres carrying different fluorescence colors or intensities for multiplex detection research.

Can SHBC provide samples and bulk quantities?

Yes. SHBC supports research samples, pilot-scale evaluation and bulk manufacturing.

Can the product be customized?

Yes. Particle size, fluorescence wavelength, surface group, solids content, suspension buffer and packaging can be customized.

Ordering Information

Product Name: 200nm Carboxyl Blue-Fluo Microspheres
Catalog Number: CFB200-10
Particle Size: 200nm
Solids Content: 1%
Excitation Wavelength: 366nm
Emission Wavelength: 460nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.

Contact SHBC for samples, technical documents, customized specifications and bulk production quotations.

For research and manufacturing use. Performance in the final application should be validated by the customer.